Dual function of an
            <i>Arabidopsis</i>
            transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression

Sakuma, Yoh; Maruyama, Kyonoshin; Qin, Feng; Osakabe, Yuriko; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko

doi:10.1073/pnas.0605639103

Cited by 702 publications

(628 citation statements)

References 43 publications

(68 reference statements)

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“…For example, overexpression of DREB2A in transgenic plants could not improve stress tolerance, but overexpression of DREB2ACA, carrying a deletion of the central negative regulatory domain (NRD), activated the expression of many stress-inducible genes and improved tolerance to drought and heat stress in transgenic Arabidopsis (Sakuma et al, 2006a(Sakuma et al, , 2006b. Similarly, AREB1, a homologous gene of OsbZIP46, has been reported to fully function after posttranslational modification (Fujita et al, 2005).…”

Section: Constitutive Activation Is a Useful Tool For Functional Analmentioning

confidence: 99%

Constitutive Activation of Transcription Factor OsbZIP46 Improves Drought Tolerance in Rice

et al. 2012

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OsbZIP46 is one member of the third subfamily of bZIP transcription factors in rice (Oryza sativa). It has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidopsis thaliana) and rice, respectively. Expression of OsbZIP46 was strongly induced by drought, heat, hydrogen peroxide, and abscisic acid (ABA) treatment; however, it was not induced by salt and cold stresses. Overexpression of the native OsbZIP46 gene increased ABA sensitivity but had no positive effect on drought resistance. The activation domain of OsbZIP46 was defined by a series of deletions, and a region (domain D) was identified as having a negative effect on the activation. We produced a constitutive active form of OsbZIP46 (OsbZIP46CA1) with a deletion of domain D. Overexpression of OsbZIP46CA1 in rice significantly increased tolerance to drought and osmotic stresses. Gene chip analysis of the two overexpressors (native OsbZIP46 and the constitutive active form OsbZIP46CA1) revealed that a large number of stress-related genes, many of them predicted to be downstream genes of ABF/ AREBs, were activated in the OsbZIP46CA1 overexpressor but not (even down-regulated) in the OsbZIP46 overexpressor. OsbZIP46 can interact with homologs of SnRK2 protein kinases that phosphorylate ABFs in Arabidopsis. These results suggest that OsbZIP46 is a positive regulator of ABA signaling and drought stress tolerance of rice depending on its activation. The stress-related genes activated by OsbZIP46CA1 are largely different from those activated by the other rice ABF/AREB homologs (such as OsbZIP23), further implying the value of OsbZIP46CA1 in genetic engineering of drought tolerance.

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Section: Constitutive Activation Is a Useful Tool For Functional Analmentioning

confidence: 99%

Constitutive Activation of Transcription Factor OsbZIP46 Improves Drought Tolerance in Rice

et al. 2012

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“…DREB2A expression is induced rapidly by HS but only gradually induced by drought stress. 8 In eukaryotic cells, the control of gene expression is determined by transcription and by post-transcriptional control. 9 Eukaryotic cells under stressful conditions halt translation initiation and induce formation of cytoplasmic granular structures formed by RNA-protein complexes, which are referred to as stress granules (SGs) or processing bodies (P-bodies).…”

Section: Introductionmentioning

confidence: 99%

Vascular plant one-zinc-finger protein 2 is localized both to the nucleus and stress granules under heat stress in Arabidopsis

Koguchi

Yamasaki

Hirano

et al. 2017

Plant Signaling & Behavior

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VASCULAR PLANT ONE-ZINC FINGER (VOZ)1/and VOZ2 have an ability to bind to the specific cis-element in the AVP1 promoter of Arabidopsis, which function on the PhyB-dependent flowering and possibly in various stress responses as potential transcription factors, although nuclear localization of VOZ proteins is still unclear. In this study, we found that VOZ2 is dispersed throughout the cytoplasm under normal growth conditions, whereas VOZ2 is transferred not only to the nucleus but also to the cytoplasmic foci under heat stress conditions. The VOZ2 foci predominantly co-localized with a marker of stress granules (SGs), which were cytoplasmic granular structures for mRNA storage and decay under abiotic stress conditions. We also demonstrated that GFP-VOZ2 with a nuclear localization signal was rapidly degraded via the ubiquitin/proteasome pathway under the heat stress conditions. Also, stress-related expression of DREB2A in the voz1voz2 mutant was significantly upregulated by heat stress as compared with that in the wild-type Arabidopsis. Our results suggest that VOZ2 is localized to SGs and nucleus under heat stress conditions, and functions as a transcriptional repressor of DREB2A in Arabidopsis.

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“…It is a member of the DREB subfamily within the APETALA2/ethylene responsive-element binding factor (AP2/ERF) family of transcription factors and contains a single conserved DNA-binding domain (Liu et al, 1998;Sakuma et al, 2002). Its expression is induced by dehydration, high salinity, and heat shock (Liu et al, 1998;Sakuma et al, 2006b). The transcription of DREB2A in response to dehydration and heat shock is independently regulated by different regions in the DREB2A promoter Yoshida et al, 2011).…”

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confidence: 99%

GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-Type Transcription Factor in Soybean, Is Posttranslationally Regulated and Mediates Dehydration-Responsive Element-Dependent Gene Expression

et al. 2012

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Soybean (Glycine max) is an important crop around the world. Abiotic stress conditions, such as drought and heat, adversely affect its survival, growth, and production. The DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2 (DREB2) group includes transcription factors that contribute to drought and heat stress tolerance by activating transcription through the cis-element dehydration-responsive element (DRE) in response to these stress stimuli. Two modes of regulation, transcriptional and posttranslational, are important for the activation of gene expression by DREB2A in Arabidopsis (Arabidopsis thaliana). However, the regulatory system of DREB2 in soybean is not clear. We identified a new soybean DREB2 gene, GmDREB2A;2, that was highly induced not only by dehydration and heat but also by low temperature. GmDREB2A;2 exhibited a high transactivation activity via DRE and has a serine/threonine-rich region, which corresponds to a negative regulatory domain of DREB2A that is involved in its posttranslational regulation, including destabilization. Despite the partial similarity between these sequences, the activity and stability of the GmDREB2A;2 protein were enhanced by removal of the serine/threonine-rich region in both Arabidopsis and soybean protoplasts, suggestive of a conserved regulatory mechanism that involves the recognition of serine/ threonine-rich sequences with a specific pattern. The heterologous expression of GmDREB2A;2 in Arabidopsis induced DREregulated stress-inducible genes and improved stress tolerance. However, there were variations in the growth phenotypes of the transgenic Arabidopsis, the induced genes, and their induction ratios between GmDREB2A;2 and DREB2A. Therefore, the basic function and regulatory machinery of DREB2 have been maintained between Arabidopsis and soybean, although differentiation has also occurred.

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Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression

Cited by 702 publications

References 43 publications

Constitutive Activation of Transcription Factor OsbZIP46 Improves Drought Tolerance in Rice

Constitutive Activation of Transcription Factor OsbZIP46 Improves Drought Tolerance in Rice

Vascular plant one-zinc-finger protein 2 is localized both to the nucleus and stress granules under heat stress in Arabidopsis

GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-Type Transcription Factor in Soybean, Is Posttranslationally Regulated and Mediates Dehydration-Responsive Element-Dependent Gene Expression

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