Dual estrogenic regulation of the nuclear progestin receptor and spermatogonial renewal during gilthead seabream (Sparus aurata) spermatogenesis

Chauvigné, François; Parhi, Janmejay; Ollé, Judith; Cerdà, Joan

doi:10.1016/j.cbpa.2017.01.008

Cited by 18 publications

(18 citation statements)

References 59 publications

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“…Oestrogen significantly influenced the maturation and differentiation of spermatocytes into spermatids 26,28 and significantly enhanced the sperm fertilising capacity in vitro , inducing the acrosome reaction and sperm capacitation 29 in mammals. For teleost, such as the European eel ( Anguilla Anguilla ) 13 , githead seabream ( Sparus aurata ) 30 , and rainbow darter ( Etheostoma caeruleum ) 31 , ER have been reported to be involved in the early stages of spermatogenic cell development. The significant up-regulation of the ER and DEG enrichment in the oestrogen pathway at the MSV stage indicated the high importance of E2 in sperm maturation and hydration in turbot.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation

Liu

Xiao

et al. 2018

Sci Rep

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Spermatogenesis is a dynamic developmental process in which spermatogonial stem cells proliferate, differentiate and mature into functional spermatozoa. These processes require an accurate gene regulation network. Here, we investigated the dynamic changes that occur during spermatogenesis through a combination of histological and transcriptome analyses of different developmental stages of the testis. We constructed 18 testis transcriptome libraries, and the average length, N50, and GC content of the unigenes were 1,795 bp; 3,240 bp and 49.25%, respectively. Differentially expressed genes (DEGs) that were related to germ cell proliferation and maturation, such as NANOS3, RARs, KIFs, steroid hormone synthesis-related genes and receptor genes, were identified between pairs of testis at different developmental stages. Gene ontology annotation and pathway analyses were conducted on DEGs with specific expression patterns involved in the regulation of spermatogenesis. Nine important pathways such as steroid hormone biosynthesis related to spermatogenesis were identified. A total of 21 modules that ranged from 49 to 7,448 genes were designed by a weighted gene co-expression network analysis. Furthermore, a total of 83 candidate miRNA were identified by computational methods. Our study provides the first transcriptomic evidence for differences in gene expression between different developmental stages of spermatogenesis in turbot (Scophthalmus maximus).

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Section: Discussionmentioning

confidence: 99%

Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation

Liu

Xiao

et al. 2018

Sci Rep

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“…21 The expression of PGR in Leydig cells has been reported in human, nonhuman primates, mouse, Japanese eel, and gilthead seabream. 31 Therefore, the effects of progestins on testis seem to be mediated through PGR in Leydig cells, and Leydig cell products, in turn, may affect spermatogenesis. In mammals, progestins inhibit spermatogenesis and sperm vitality, [32][33][34] and accordingly, the KO of the PGR increases testicular development and sperm production.…”

Section: Discussionmentioning

confidence: 99%

GFP expression pattern in pituitary and gonads under the control of nuclear progesterone receptor promoter in transgenic zebrafish

Huang

Zhang

Jiang

et al. 2020

Developmental Dynamics

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Background: The nuclear progesterone receptor (Pgr) is a ligand-dependent transcription factor primarily responsible for mediating progesterone actions relevant for reproduction across vertebrates. Information on the cellular localization of Pgr expression in the reproductive system is required for developing a comprehensive approach to elucidate the role of Pgr in reproduction. Results: We generated transgenic zebrafish Tg(pgr:eGFP) that express enhanced green fluorescent protein (eGFP) driven by promoter sequence of pgr gene. The tissue distribution pattern of egfp mRNA is consistent with the pgr mRNA expression in Tg(pgr:eGFP). In the pituitary, GFP signals are found in the proximal pars distalis. In order to better discern the cellular localization of GFP signals in gonads, Tg(pgr:eGFP) line was crossed with Tg(gsdf:nfsB-mCherry) line, specifically expressing nitroreductase-mCherry fusion protein in granulosa and Sertoli cells in ovary and testis, respectively. Imaging of testis tissue showed that GFP expression was confined to Leydig cells. In the ovary, GFP expression colocalized with the mCherry signal in granulosa cells. Intriguingly, we also identified some non-granulosa cells close to where blood vessels branched, expressing stronger GFP signals than granulosa cells. Conclusions: Analyzing Tg(pgr:eGFP) expression in zebrafish provided leads toward new routes to study the role of Pgr in reproduction.

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“…e ERc form in fish appears to be closely related to ERβ which suggests that a gene replication event has transpired within the teleosts. As a result, ERβ and ERc have been named ERβ1 and ERβ2 [1,4,5].…”

Section: Estrogen Receptorsmentioning

confidence: 99%

“…Local production of steroid hormones in the brain and other tissues of importance is of increasing interest concerning control of numerous behavioral and physiological processes from sexual behavior to brain sex differentiation in a variety of species [3]. While mammals have one gene for double estrogen receptor subtypes (ESR1 and ESR2), in teleost fishes, three estrogen receptors of the regulatory transcription factor type have been acknowledged, a single ERα and two ERβ1 and ERβ2 genes [4,5]. Estrogen is renowned for its involvement in the dictate of "oogenesis, vitellogenesis, gonadotropin regulation, testicular growth, and other facets of regeneration, in addition to having controlling roles in several structure organizations."…”

Section: Introductionmentioning

confidence: 99%

“…Yet again, estrogen impedes osteopenia and is therefore beneficial in the postmenopausal female disposed to osteoporosis. Estrogen essentially binds to its cognate receptor to exert its effects, and in animals, two categories of estrogen receptors (ERs), alpha and beta, exist [4,5]. A third ER, ERc, has been discovered for teleost fish [18].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Review on Sex Steroid Hormone Estrogen Receptors in Mammals and Fish

Amenyogbe

Chen

Wang

et al. 2020

International Journal of Endocrinology

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Steroid hormones play essential roles in the reproductive biology of vertebrates. Estrogen exercises its effect through estrogen receptors and is not only a female reproductive hormone but acts virtually in all vertebrates, including fish, and is involved in the physiological and pathological states in all males and females. Estrogen has been implicated in mandible conservation and circulatory and central nervous systems as well as the reproductive system. is review intended to understand the structure, function, binding affinities, and activations of estrogens and estrogen receptors and to discuss the understanding of the role of sex steroid hormone estrogen receptors in mammals and fish.

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Dual estrogenic regulation of the nuclear progestin receptor and spermatogonial renewal during gilthead seabream (Sparus aurata) spermatogenesis

Cited by 18 publications

References 59 publications

Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation

Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation

GFP expression pattern in pituitary and gonads under the control of nuclear progesterone receptor promoter in transgenic zebrafish

A Review on Sex Steroid Hormone Estrogen Receptors in Mammals and Fish

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