Dual effects of nitric oxide in functional and regressing rat corpus luteum

Motta, Alicia Beatriz; Estévez, Alejandra; Tognetti, T.; Gimeno, M.A.F.; Franchi, A.M.

doi:10.1093/molehr/7.1.43

Cited by 58 publications

(71 citation statements)

References 26 publications

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“…The reasons of rise may be increasing follicular diameters at 17β-estradiol levels, starting corpus luteum development at progesterone levels. The finding of increase NO levels supports the findings of other studies (22,26) which report that as the cycle progresses, sensitivity to NO increases and more NO is synthesized in the luteinized ovarium. In the luteal period, increase in the flow of blood ensures the formation of corpus luteum and maintains its functional characteristics (21).…”

Section: Discussionsupporting

confidence: 90%

Blood nitric oxide and ovarian steroids levels during the cycle stages in Brown Swiss cows

ÇELİK¹

2008

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The purpose of this study was to examine the fluctuations of the nitric oxide (NO) levels during the estrus cycle stages of cows and the association of these variations with ovarian steroids. In this study eighteen Brown Swiss cows were used. The ovarian activities of the cows were determined by rectal and ultrasound examinations. The estruses of the cows were synchronized by double dose prostaglandin F 2α (PGF 2α ) administered with 11-day intervals. The blood samples of the cows in the cycle stages were taken, and NO, estradiol and progesterone values were measured. Highest estradiol concentration was determined during estrus stage (p<0.05). Progesterone was the lowest in estrus, whereas it was the highest in midcycle stage (p<0.05). The highest NO value was measured during the estrus (p<0.05) and the lowest NO value in the metestrus (p<0.05). In conclusion, the variations was observed in the NO level in different cycle periods in Brown Swiss cows, and the NO was found to be in highest concentration in proestrus and estrus periods like 17β-estradiol Keywords: Cow, estradiol, estrus cycle, nitric oxide, progesterone İsviçre Esmeri ineklerin siklus dönemlerinde kan nitrik oksit ve ovaryum steroid düzeyleriÖzet: Çalışmada İsviçre Esmeri ineklerde östrüs siklusunun dönemlerinde kan nitrik oksit ve ovaryum steroidlerinin düzeylerinin belirlenmesi amaçlanmıştır. Çalışmada 18 İsviçre Esmeri inek kullanıldı. Rektal ve ultrason muayeneleri ile ineklerin ovaryum aktiviteleri belirlendi. Hayvanların östrüsleri 11 gün ara ile çift doz PGF2α uygulanarak senkronize edildi. Siklusun dönemlerinde kan örnekleri alınarak nitrik oksit (NO), östradiol ve progesteron analizleri gerçekleştirildi. En yüksek östradiol düzeyi östrüs döneminde belirlendi (p<0.05). Progesteron düzeyi östrüs döneminde en düşük; erken, orta ve geç diöstrus dönemlerinde ise en yüksek ölçüldü (p<0.05). Nitrik oksit en yüksek östrüs döneminde, en düşük ise metöstrüs döneminde belirlendi. Sonuç olarak İsviçre Esmeri ineklerde kan NO düzeyinin siklusun dönemlerinde değiştiği ve 17β-östradiolün en yüksek düzeyde bulunduğu proöstrus ve östrus dönemlerinde NO'nun da yüksek olduğu belirlenmiştir.

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Section: Discussionsupporting

confidence: 90%

Blood nitric oxide and ovarian steroids levels during the cycle stages in Brown Swiss cows

ÇELİK¹

2008

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…Further, we did not look at other NOS isoforms including neuronal or inducible NOS. However, we do hypothesize that one or both synthases may play a role in progesterone decrease, as NO has been proposed to regulate luteal steroidogenesis PGF 2a -induced luteal regression in sheep (Van Voorhis et al 1994, Jaroszewski & Hansel 2000, Motta et al 2001.…”

Section: Pgf 2a -Induced Luteal Regression In Sheepmentioning

confidence: 91%

“…Determining the profile of angiogenic factors responsible for vascular changes may prove to be important in determining how the composition of the CL is altered during regression. While there are several factors which have been shown to influence vascularity of different tissues, some of the primary regulators of ovarian angiogenesis and vascular function include: the vascular endothelial growth factor (VEGF) system (including the VEGF receptors and the neuropilins; Ferrara et al 1992, Shalaby et al 1995, Neufeld et al 1999, Stacker & Achen 1999, fibroblastic growth factors (FGF) and receptors (Klagsbrun & D'Amore 1991, Neuvians et al 2004a, the angiopoietins (ANGPT) and their receptor Tie-2 (Sato et al 1993, Davis & Yancopoulos 1999, Hazzard et al 2000, Wulff et al 2000, Tanaka et al 2004, the nitric oxide (NO) system (Motta et al 2001, Klipper et al 2004, Shi et al 2004, Al-Gubory et al 2005, and the renin-angiotensin system (Hansel & Blair 1996, Hayashi & Miyamoto 1999, Kobayashi et al 2001. While it is most certain that there are several other angiogenic factors playing a role in the regulation of vascular function, selected members from these families were investigated in the current experiment.…”

Section: Introductionmentioning

confidence: 99%

Vascular composition, apoptosis, and expression of angiogenic factors in the corpus luteum during prostaglandin F2α-induced regression in sheep

Vonnahme¹,

Redmer²,

Borowczyk³

et al. 2006

Reproduction

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Corpora lutea and blood samples were collected from superovulated ewes 0, 4, 8, 12 and 24 h after prostaglandin F 2a (PGF) analog injection on day 10 of the estrous cycle. Changes in vascular cell and fibroblast composition, apoptosis and mRNA expression for several angiogenic factors in the corpus luteum (CL) were determined. While peripheral progesterone concentration decreased at 24 h after PGF injection, CL weight did not change. The area of positive BS-1 lectin staining (endothelial cell marker), smooth muscle cell actin (SMCA; pericyte and SMC marker), collagen type 1 (fibroblast marker), and the rate of cell death changed in luteal tissues after PGF treatment. In association with these cellular changes, mRNA for several angiogenic factors including vascular endothelial growth factor (VEGF) and receptors (Flt and KDR), basic fibroblast growth factor (FGF2) and receptor, angiopoietin (ANGPT) 1 and receptor Tie-2, endothelial nitric oxide synthase (NOS3), and angiotensin II receptor 1 (AT1) were altered. Changes in endothelial cell marker expression were positively correlated with changes in VEGF and NO systems. In addition, changes in mRNA expression for VEGF, Flt and KDR were positively correlated with changes in ANGPT2, Tie-2, and NOS3, indicating a functional relationship. This data demonstrates that after an initial increase, the endothelial component of the vascular bed decreases during PGF-induced luteal regression. However, SMCA expression remained high during luteal regression, potentially indicating a role of pericytes and vascular SMC in luteolysis, likely to regulate tissue remodeling and to maintain the integrity of larger blood vessels. Further, it appears that early regression may increase collagen type 1 production and/or expression by fibroblasts. Expression of angiogenic factors is influenced by PGF-induced luteolysis and may serve to maintain vascular structure in order to aid luteal regression.

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“…GSH is an antioxidant metabolite involved in reactions of oxide reduction, thus neutralizing oxidant species. It has been reported that antioxidant defenses are regulated by hormones (Motta & Gimeno 1997, Motta et al 2001a, 2001b, Tam et al 2003, Estevez et al 2004, Duarte et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Effect of DHEA and metformin on corpus luteum in mice

Sander¹,

Facorro²,

Piehl³

et al. 2009

Reproduction

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We evaluated the effect of hyperandrogenism in ovaries with functional and regressing corpora lutea (CL) and the action of metformin in preventing these possible alterations using a mouse model. To obtain a CL functional for 9G1 days, immature female mice of the BALB/c strain were injected i.p. with 10 IU/mouse of pregnant mare's serum gonadotropin (PMSG). DHEA (60 mg/kg body weight s.c., 24 and 48 h prior to kill) decreased both serum progesterone (P) and estradiol (E 2 ) levels and increased the activity of superoxide dismutase (SOD) from ovaries with functional CL (on day 5 after PMSG). It increased P and E 2 and the activities of SOD and catalase (CAT) and decreased lipoperoxidation of ovaries with regressing CL (on day 9 after PMSG). Treatment with DHEA did not affect the production of prostaglandin F 2a (PGF 2a ) or PGE by ovaries with functional CL, whereas DHEA decreased PGF 2a and increased PGE production by ovaries with regressing CL. Metformin (50 mg/kg body weight, orally) given together with DHEA restored E 2 levels from mice with ovaries with functional CL and serum P, PGF 2a and PGE levels, and oxidative balance in mice with ovaries with regressing CL. Metformin alone was able to modulate serum P and E 2 levels, lipoperoxidation, SOD and CAT, and the 5,5-dimethyl-1-pyrroline N-oxide/ % OH signal. These findings suggest that hyperandrogenism is able to induce or to rescue CL from luteolysis and metformin treatment is able to prevent these effects.

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Dual effects of nitric oxide in functional and regressing rat corpus luteum

Cited by 58 publications

References 26 publications

Blood nitric oxide and ovarian steroids levels during the cycle stages in Brown Swiss cows

Blood nitric oxide and ovarian steroids levels during the cycle stages in Brown Swiss cows

Vascular composition, apoptosis, and expression of angiogenic factors in the corpus luteum during prostaglandin F2α-induced regression in sheep

Effect of DHEA and metformin on corpus luteum in mice

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