Dual effect of muscarinic receptor agonists on Ca2+ mobilization in SH-SY5Y neuroblastoma cells

Järv, Jaak; Hautala, Rasmus; Åkerman, Karl E.O.

doi:10.1016/0922-4106(95)90187-6

Cited by 10 publications

(6 citation statements)

References 15 publications

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“…Indeed, there was no influence of 5-HT on G␣ i3 activation in nontransfected CHO cells and the entire bell-shaped isotherms of 5-HT and the selective 5-HT 1A receptor agonist (ϩ)-8-OH-DPAT were right-shifted by the selective antagonist WAY100,635 (Table 4; Fletcher et al, 1996;Newman-Tancredi et al, 1997). Hence, the presence of multiple receptors can be ruled out, although the possibility remains that agonists may be sequentially acting at multiple binding sites on target receptors, as has been proposed for bell-shaped mobilization of calcium ions by muscarinic agonists in SH-SY5Y neuroblastoma cells (Jä rv et al, 1995). However, that study did not report whether agonist actions could be blocked by antagonists.…”

Section: Discussionmentioning

confidence: 92%

Antibody Capture Assay Reveals Bell-Shaped Concentration-Response Isotherms for h5-HT_1AReceptor-Mediated Gα_i3Activation: Conformational Selection by High-Efficacy Agonists, and Relationship to Trafficking of Receptor Signaling

et al. 2002

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Although serotonin 5-HT 1A receptors couple to several Gi/o Gprotein subtypes, little is known concerning their differential activation patterns. In this study, in membranes of Chinese hamster ovary cells expressing h5-hydroxytryptamine 1A receptors (CHOh5-HT 1A ), isotherms of 5-HT-stimulated guanosine-5Ј-O-(3-[ , and iii) absent in nontransfected CHO cell membranes. 5-HT isotherms could be modified by altering sodium concentration; only stimulatory actions were observed at 300mM NaCl, whereas only inhibitory actions were seen at 10 mM NaCl. Furthermore, bell-shaped isotherms were not detected at short incubation times, suggesting time-dependent changes in receptor/G␣ i3 coupling. Taken together, these data show that low but not high concentrations of high-efficacy 5-HT 1A agonists direct receptor signaling to G␣ i3 . In contrast, partial agonists favor h5-HT 1A receptor signaling to G␣ i3 over a wide concentration range, whereas inverse agonists inhibit constitutive G␣ i3 activation.

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Section: Discussionmentioning

confidence: 92%

Antibody Capture Assay Reveals Bell-Shaped Concentration-Response Isotherms for h5-HT_1AReceptor-Mediated Gα_i3Activation: Conformational Selection by High-Efficacy Agonists, and Relationship to Trafficking of Receptor Signaling

et al. 2002

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“…These receptors reveal substantial structural homology with other 7-TM receptors (3), which, in turn, suggests the possibility of similar functional aspects. In this report we have examined the ability of the P2Y receptors to produce the bell-shaped dose-response curves previously observed with some other G-protein-coupled receptors, a nding most thoroughly analysed for muscarinic receptor-regulated cAMP synthesis (4) and for the mobilisation of intracellular Ca 2+ by muscarinic agonists (5). The dual effect of nucleotides was observed for both a purine-speci c and a pyrimidine-speci c receptor.…”

Section: Introductionmentioning

confidence: 88%

Dual Effect of Nucleotides on P2Y Receptors

Sak¹,

Barnard²,

Järv³

2000

IUBMB Life

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The interaction of ADP, 2MeSADP, and ADPbetaS with the adenine nucleotide receptor P2Y1 in the hP2Y1-1321N1 cell line and of UDP with a receptor or receptors recognizing pyrimidine nucleotides in NG108-15 cells was studied over a wide range ofligand concentrations. Bell-shaped dose-response curves for stimulation of phosphoinositide hydrolysis were obtained in these cells. This dual behavior of the agonists studied was characterized by two dissociation constants, K(agon) and K(antag), which quantify the agonistic and antagonistic activity of these ligands and can be compared with the conventional EC50 and IC50 values, respectively. The data revealed a common pattern of agonistic and antagonistic behavior of nucleoside diphosphates and their derivatives at these two types of P2Y receptors, pointing to some similar properties of their nucleotide binding sites.

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“…(21) Receptor with high affinity to the agonist but have lower capacity (fewer receptors) mediate the stimulation, and receptor with lower affinity but have a greater capacity mediate the inhibitory response. (22)(23)(24)(25) From this concept, numerous receptors that mediated biphasic dose/concentration responses were identified since 1980s with many examples that conformed to the hormetic dose/concentration response quantitative features. (23,24) The mechanism involving a complex and integrative array of signal transduction pathways.…”

Section: Hormetic Mechanismsmentioning

confidence: 99%

Hormesis in Health and Disease: Molecular Mechanisms

Meiliana

Wijaya

2020

Indones Biomed J

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BACKGROUND: Hormesis was initially defined as a phenomenon where a small dose of harmful agent exposure to living organisms gives beneficial effects. The dose and time of this ‘tress’ exposure has become the object of investigation across the broad range of biomedical studies.CONTENT: Hormesis characterized by the biphasic dose-effect or time-effect relationship for any substance. Some hormetic mechanisms performed biological plasticity, involve oxidative damage which instead induce antioxidant enzyme production in various cells. Early-life stress can increase resilience in later life and lack of stress can lead to vulnerability. Many stressors like dietary factors and natural environmental toxins can be occupied for healthy growth or homeostasis, which exemplifies how illness is the doorway to health.SUMMARY: Hormesis reconcile many paradoxical phenomena exert opposite effects of the same substance, either a xenobiotic or an endogenous substance, a hormone or a metabolite, a genetic manipulation or an epigenetic alteration, an experimental intervention or a natural event. Human bodies are highly adaptive. A resilient body would be resulted after the ‘training’. In this review, we will elucidate the hormesis’ definition, mechanisms and pathways, and also how hormesis impacts in human health and lifespan.KEYWORDS: biphasic, cell signaling, dose response, hormesis, preconditioning

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Dual effect of muscarinic receptor agonists on Ca2+ mobilization in SH-SY5Y neuroblastoma cells

Cited by 10 publications

References 15 publications

Antibody Capture Assay Reveals Bell-Shaped Concentration-Response Isotherms for h5-HT_1AReceptor-Mediated Gα_i3Activation: Conformational Selection by High-Efficacy Agonists, and Relationship to Trafficking of Receptor Signaling

Antibody Capture Assay Reveals Bell-Shaped Concentration-Response Isotherms for h5-HT_1AReceptor-Mediated Gα_i3Activation: Conformational Selection by High-Efficacy Agonists, and Relationship to Trafficking of Receptor Signaling

Dual Effect of Nucleotides on P2Y Receptors

Hormesis in Health and Disease: Molecular Mechanisms

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Dual effect of muscarinic receptor agonists on Ca2+ mobilization in SH-SY5Y neuroblastoma cells

Cited by 10 publications

References 15 publications

Antibody Capture Assay Reveals Bell-Shaped Concentration-Response Isotherms for h5-HT1AReceptor-Mediated Gαi3Activation: Conformational Selection by High-Efficacy Agonists, and Relationship to Trafficking of Receptor Signaling

Antibody Capture Assay Reveals Bell-Shaped Concentration-Response Isotherms for h5-HT1AReceptor-Mediated Gαi3Activation: Conformational Selection by High-Efficacy Agonists, and Relationship to Trafficking of Receptor Signaling

Dual Effect of Nucleotides on P2Y Receptors

Hormesis in Health and Disease: Molecular Mechanisms

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Product

Resources

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Antibody Capture Assay Reveals Bell-Shaped Concentration-Response Isotherms for h5-HT_1AReceptor-Mediated Gα_i3Activation: Conformational Selection by High-Efficacy Agonists, and Relationship to Trafficking of Receptor Signaling

Antibody Capture Assay Reveals Bell-Shaped Concentration-Response Isotherms for h5-HT_1AReceptor-Mediated Gα_i3Activation: Conformational Selection by High-Efficacy Agonists, and Relationship to Trafficking of Receptor Signaling