Dual effect of insulin resistance and cadmium on human granulosa cells - In vitro study

Belani, Muskaan; Shah, Preeti; Banker, Manish; Gupta, Sarita

doi:10.1016/j.taap.2016.10.019

Cited by 18 publications

(10 citation statements)

References 42 publications

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“…In the ovary, granulosa cells are responsible for providing energy substrates and intermediates such as pyruvate to the oocytes, and normal glucose metabolism in the granulosa cells is essential for maturation, developmental competency, and protection of oocytes (28). Thus, IR of granulosa cells may impair the oocytes' development potential and the function of granulosa cells (29,30). Previous studies demonstrated that human granulosa cells from patients with PCOS showed reduced glucose uptake (7,31), which is consistent with our study that hGLs from patients with PCOS with IR showed reduced glucose uptake upon insulin stimulation.…”

Section: Discussionmentioning

confidence: 99%

Elevated chemerin induces insulin resistance in human granulosa‐lutein cells from polycystic ovary syndrome patients

Zhu

Wang

et al. 2019

FASEB j.

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The insulin resistance (IR) of ovarian granulosa cells from polycystic ovary syndrome (PCOS) aggravates the abnormalities in steroidogenesis and anovulation, and chemerin is an adipokine involved in regulating adipogenesis and glucose homeostasis. The role and underlying mechanism of chemerin in developing IR of the granulosa cells from PCOS remain unclear. Plasma, follicular fluid, and human granulosa‐lutein cells (hGLs) were collected from non‐PCOS and patients with PCOS with or without IR. The chemerin levels were elevated in both follicular fluid and hGL samples from patients with PCOS with IR, and the hGLs from patients with PCOS with IR showed decreased insulin sensitivity and impaired glucose uptake capacity. Moreover, treatment of chemerin attenuated insulin‐stimulated glucose uptake by decreasing phosphorylation of insulin receptor substrate (IRS)1/2 Tyr612, phosphorylation of protein kinase B Ser473, and membrane translocation of glucose transporter type 4 through increasing Ser307 phosphorylation of IRS1 in cultured hGLs. These effects could be abolished by small interfering RNA‐mediated knockdown of chemokine‐like receptor 1. Furthermore, insulin induced the expression of chemerin in hGLs. Our findings demonstrate a novel role of chemerin in the metabolic dysfunction of PCOS, which suggested that chemerin and its receptor can be further implicated as potential therapeutic targets in the future treatment of PCOS.—Li, X., Zhu, Q., Wang, W., Qi, J., He, Y., Wang, Y., Lu, Y., Wu, H., Ding, Y., Sun, Y. Elevated chemerin induces insulin resistance in human granulosa‐lutein cells from polycystic ovary syndrome patients. FASEB J. 33, 11303–11313 (2019). http://www.fasebj.org

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Section: Discussionmentioning

confidence: 99%

Elevated chemerin induces insulin resistance in human granulosa‐lutein cells from polycystic ovary syndrome patients

Zhu

Wang

et al. 2019

FASEB j.

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“…Periovulatory insulin signaling is essential for ovulation, cell differentiation of the granulosa, and female fertility (7). An in vitro study found that IR could decrease steroidogenesis and increase apoptosis of human granulosa cells (8). Preimplantation exposure to high concentrations of insulinlike growth factor I results in lower implantation rates in mice (9).…”

Section: Introductionmentioning

confidence: 99%

Effect of Hyperinsulinemia and Insulin Resistance on Endocrine, Metabolic, and Reproductive Outcomes in Non-PCOS Women Undergoing Assisted Reproduction: A Retrospective Cohort Study

et al. 2022

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Objective: To evaluate the effect of hyperinsulinemia (HI) and insulin resistance (IR) on endocrine, metabolic, and reproductive outcomes in women without polycystic ovary syndrome (PCOS) undergoing assisted reproduction.Materials and Methods: The study included 1,104 non-PCOS women undergoing in vitro fertilization/intracytoplasmic sperm injection-fresh embryo transfer. HI was evaluated by serum fasting insulin (FIN), and IR was evaluated by homeostatic model assessment of insulin resistance index (HOMA-IR). In addition, biometric, sex hormone, and metabolic parameters were measured. Independent t-test, linear, and logistic regression examined associations between HI, IR, and endocrine, metabolic, ovarian stimulation characteristics, and reproductive outcomes.Results: Women with HI and IR had lower levels of progesterone, luteinizing hormone, follicle-stimulating hormone, estradiol, high-density lipoproteins, and increased levels of triglycerides low-density lipoproteins. For ovarian stimulation characteristics, those with HI and IR had a longer duration of stimulation, a higher total gonadotropin dose, and a lower peak estradiol level. Linear regression confirmed these associations. For reproductive outcomes, HI and IR were not associated with clinical pregnancy, live birth, and miscarriage.Conclusions: HI and IR did not impair reproductive outcomes in non-PCOS women undergoing assisted reproduction.

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“…Cd could affect these enzymes in the steroidogenesis pathway. Previous studies have shown that a high concentration of Cd decreases the concentration of E2 and P4 in human granulosa cells by downregulation of StAR, CYP11A1, and CYP19A1 levels, in vitro (Belani et al, 2016; Zhang and Jia, 2007). In addition, exposure to high doses of Cd in porcine granulosa cells inhibited the expression of P450scc gene and P4 synthesis (Smida et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Impaired follicular development and endocrine disorders in female rats by prepubertal exposure to toxic doses of cadmium

et al. 2020

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Cadmium (Cd) has been associated with several physiological problems including reproductive and endocrine system dysfunction resulting in temporary infertility. The principal objective of this project was to investigate the effects of prepubertal exposure to toxic doses of Cd on puberty onset, the endocrine system, and follicular development. For this purpose, 16 female Sprague-Dawley rats weaned on postnatal day (PND) 21 were randomly divided into 4 groups ( n = 4 per group). The treatments were as follows: 0, 25, 50, and 75 mg/kg/day of cadmium chloride (CdCl2) by oral gavage from PND 21 to observation of first vaginal opening (VO). The results demonstrated that prepubertal exposure to different doses of CdCl2 delays the age of VO, first diestrus, and first proestrus via altering the concentrations of estradiol and progesterone. The low level of these steroid hormones contributed to lower differentiation and maturation of follicles and it finally led to reduced ovarian reservoir of follicles and impaired follicular development. The number of atretic follicles and secondary follicles with premature cavity increased in rats that received a high dose of CdCl2, whereas the number of secondary follicles and corpora luteum decreased in the same circumstances. Taken together, these data suggest that prepubertal exposure to toxic doses of Cd delays the onset of puberty via disorderliness in the concentration of steroid hormones and reduces the ovarian reservoir of follicles, as well as folliculogenesis.

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Dual effect of insulin resistance and cadmium on human granulosa cells - In vitro study

Cited by 18 publications

References 42 publications

Elevated chemerin induces insulin resistance in human granulosa‐lutein cells from polycystic ovary syndrome patients

Elevated chemerin induces insulin resistance in human granulosa‐lutein cells from polycystic ovary syndrome patients

Effect of Hyperinsulinemia and Insulin Resistance on Endocrine, Metabolic, and Reproductive Outcomes in Non-PCOS Women Undergoing Assisted Reproduction: A Retrospective Cohort Study

Impaired follicular development and endocrine disorders in female rats by prepubertal exposure to toxic doses of cadmium

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