Dual design spaces for micro-extraction together with the core–shell chromatographic determination of dorzolamide and timolol in rabbit plasma: an example of quality by design method development

Abstract

An innovative quality by design-integrated VA-SALLME-core–shell chromatographic method was developed for the simultaneous determination of DOR and TIM in plasma.

Key information related to quality by design (QbD) applications in analytical methods development

“…QbD strategy was used by Mohamed et al [44] to develop a new method combining vortex-aided salting-out-assisted liquid-liquid microextraction with a core-shell HPLC method establishing an efficient, sensitive and simultaneous determination of dorzolamide hydrochloride (DOR) and timolol maleate (TIM) in rabbit plasma with high analytical frequency. Screening of the knowledge space was performed with a PB design, and a BBD was applied for RSM.…”