Dual-delivery of VEGF and PDGF by double-layered electrospun membranes for blood vessel regeneration

Zhang, Hong; Jia, Xiaoling; Han, Fengxuan; Zhao, Jin; Zhao, Yunhui; Fan, Yubo; Yuan, Xiaoyan

doi:10.1016/j.biomaterials.2012.12.005

Cited by 234 publications

(183 citation statements)

References 38 publications

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“…41,42 Therefore, we used SDF-1α and VEGF to study the bioactivity of cytokine-loaded CSO/H nanoparticles. Our results show that CSO/H nanoparticles can load not only a large amount of cytokines, but also a variety of cytokines (such as SDF-1α and VEGF, Table 2), indicating that these nanoparticles could serve as a carrier for cytokines.…”

mentioning

confidence: 99%

Novel stable cytokine delivery system in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Wang

Tan

Deng

et al. 2015

IJN

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Background Cell therapy is a promising strategy for tissue regeneration. Key to this strategy is mobilization and recruitment of exogenous or autologous stem/progenitor cells by cytokines. However, there is no effective cytokine delivery system available for clinic application, in particular for myocardial regeneration. The aim of this study was to develop a novel cytokine delivery system that is stable in solution at physiological pH. Methods Four groups of self-assembled chitosan oligosaccharide/heparin (CSO/H) nanoparticles were prepared with various volume ratios of chitosan oligosaccharide to heparin (5:2, 5:4, 4:15, 1:5) and characterized by laser diffraction, particle size analysis, and transmission electron microscopy. The encapsulation efficiency and loading content of two cytokines, ie, stromal cell-derived factor (SDF)-1α and vascular endothelial growth factor (VEGF) were quantified using an enzyme-linked immunosorbent assay. The biological activity of the loaded SDF-1α and VEGF was evaluated using the transwell migration assay and MTT assay. The dispersion profiles for the cytokine-loaded nanoparticles were quantified using fluorescence molecular tomography. Results CSO/H nanoparticles were prepared successfully in solution with physiological pH. The particle sizes in the four treatment groups were in the range of 96.2–210.5 nm and the zeta potential ranged from −29.4 mV to 24.2 mV. The loading efficiency in the CSO/H nanoparticle groups with the first three ratios was more than 90%. SDF-1α loaded into CSO/H nanoparticles retained its migration activity and VEGF loaded into CSO/H nanoparticles continued to show proliferation activity. The in vivo dispersion test showed that the CSO/H nanoparticles enabled to VEGF to accumulate locally for a longer period of time. Conclusion CSO/H nanoparticles have a high cytokine loading capacity and allow cytokines to maintain their bioactivity for longer, are stable in an environment with physiological pH, and may be a promising cytokine delivery system for tissue regeneration.

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confidence: 99%

Novel stable cytokine delivery system in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Wang

Tan

Deng

et al. 2015

IJN

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“…27 The novel biodegradable PEG-SS-PLL catiomer significantly improved transfection efficiency up to 96%, markedly higher than previous studies (81.3%). 2,39 The catiomer delivered siVEGF into tumor cells more efficiently, because aggregates formed due to reductive cleavage of the intermediate disulfide bonds and resulted in the shedding of the PEG layers. These results indicated that the PEG-SS-PLL is highly promising for controlled and efficient release of siVEGF.…”

Section: Discussionmentioning

confidence: 99%

Polyethylene glycol–poly(ε-benzyloxycarbonyl-L-lysine)-conjugated VEGF siRNA for antiangiogenic gene therapy in hepatocellular carcinoma

Wang¹,

Gao²,

Gu³

et al. 2017

IJN

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A polyethylene glycol–poly(ε-benzyloxycarbonyl- l -lysine) (PEG-SS-PLL) block copolymer based on a disulfide-linked, novel biodegradable catiomer bearing a PEG-sheddable shell was developed to avoid “PEG dilemma” in nanoparticle intracellular tracking of PEG-PLL where PEG was nondegradable. However, PEG-SS-PLL catiomers have not been used to deliver small interfering VEGF RNA (siVEGF) in antiangiogenesis gene therapy. In this study, we aimed to investigate whether this novel biodegradable catiomer can deliver siVEGF into cancer cells and at the same time have an antitumor effect in a xenograft mouse model. It was found that PEG-SS-PLL efficiently delivered siVEGF with negligible cytotoxicity, and significantly decreased the expression of VEGF at both the messenger-RNA and protein levels both in vitro and in vivo, and thus tumor growth was inhibited. Our findings demonstrated that PEG-SS-PLL/siVEGF could potentially be applied to antiangiogenesis gene therapy for hepatocellular carcinoma.

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“…20 For example, to enhance the proliferation of ECs, both vascular endothelial growth factor and plateletderived growth factor have been encapsulated in electrospun membranes with dual-delivery function. 21 Furthermore, in the process of intervention for atherosclerosis and proliferation of ECs, ZNF580 gene plays an important role in promoting the proliferation of ECs and inhibiting the proliferation of smooth muscles. 22 In our previous studies, several gene carriers to condense with pEGFP-ZNF580 gene (pEGFP-ZNF580 gene is the recombinant plasmid of enhanced GFP plasmid and ZNF580 gene) were prepared with different block copolymers for the proliferation of ECs.…”

Section: Feng Et Almentioning

confidence: 99%

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

Feng¹,

Guo²,

Li³

et al. 2016

IJN

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Dual-delivery of VEGF and PDGF by double-layered electrospun membranes for blood vessel regeneration

Cited by 234 publications

References 38 publications

Novel stable cytokine delivery system in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Novel stable cytokine delivery system in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Polyethylene glycol–poly(ε-benzyloxycarbonyl-L-lysine)-conjugated VEGF siRNA for antiangiogenic gene therapy in hepatocellular carcinoma

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

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Dual-delivery of VEGF and PDGF by double-layered electrospun membranes for blood vessel regeneration

Cited by 234 publications

References 38 publications

Novel stable cytokine delivery system&nbsp;in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Novel stable cytokine delivery system&nbsp;in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Polyethylene glycol&ndash;poly(&epsilon;-benzyloxycarbonyl-L-lysine)-conjugated VEGF siRNA for antiangiogenic gene therapy in hepatocellular carcinoma

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

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Novel stable cytokine delivery system in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Novel stable cytokine delivery system in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Polyethylene glycol–poly(ε-benzyloxycarbonyl-L-lysine)-conjugated VEGF siRNA for antiangiogenic gene therapy in hepatocellular carcinoma