Dual crosslinked hyaluronic acid nanofibrous membranes for prolonged prevention of post-surgical peritoneal adhesion

Sheu, Chialin; Shalumon, K.T.; Chen, Chih‐Hao; Kuo, Chang-Yi; Fong, Yi Teng; Chen, Jyh‐Ping

doi:10.1039/c6tb01376g

Cited by 22 publications

(14 citation statements)

References 55 publications

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“…Two further zones of weight loss begin at around 220 and 400 °C, corresponding to the decomposition of HA and DEGMA-co-BMA copolymer grafted on HA chains, respectively. Heating to 700 °C resulted in a final weight of 10 % residual weight.Similar steps of decomposition were previously observed for membranes composed of HA and polyethylene oxide (PEO), with decomposition of PEO starting at 411 °C and final resistance of HA for complete decomposition 45. Finally, TGA curves of magnetic nanohybrids were measured.…”

supporting

confidence: 65%

Synthesis and magnetic manipulation of hybrid nanobeads based on Fe3O4 nanoclusters and hyaluronic acid grafted with an ethylene glycol-based copolymer

Rippe

Michelas

Putaux

et al. 2020

Applied Surface Science

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Magnetic biopolymer hybrid nanoparticles have gained a lot of attention in the field of biomedical applications. Here we present the synthesis of hyaluronic acid (HA)-coated magnetic clusters of superparamagnetic nanoparticles. Firstly, the superparamagnetic magnetite nanoparticles coated with oleic acid were synthesized and characterized in terms of size, composition and magnetic properties. Secondly, magnetic nanoclusters were prepared and wrapped with HA grafted with an ethylene glycol-based copolymer. The resulting nanostructures and nanobeads were characterized using electron microscopy, dynamic light scattering, thermogravimetric analysis and magnetic measurements. The clustering of magnetite nanoparticles facilitates magnetic purification, separation, rapid attraction and controlled positioning by high field gradient micro-magnet arrays. In addition, clusters could be formed in the presence of a fluorescent drug model, which demonstrates the possibility of dual functionalization of our hybrid nanosystems: magnetic responsiveness and drug encapsulation.

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supporting

confidence: 65%

Synthesis and magnetic manipulation of hybrid nanobeads based on Fe3O4 nanoclusters and hyaluronic acid grafted with an ethylene glycol-based copolymer

Rippe

Michelas

Putaux

et al. 2020

Applied Surface Science

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“…Subsequently, HHA‐S was electrospun to prepare nanofibers, FHHA‐S, which were further crosslinked by Fe 3+ . [ 16 ] In the FTIR spectrum of FHHA‐S/Fe, appearance of a new peak at 1728 cm −1 indicates the coordination bond formation between Fe 3+ and the carboxyl groups of HHA (Figure S4, Supporting Information), indicating the Fe 3+ crosslinking. For comparison, FHHA and FHHA/Fe without thioether groups were also prepared.…”

Section: Resultsmentioning

confidence: 99%

“…Crosslinked nanofibers were prepared by the solution immersion method. [ 16 ] Briefly, FHHA and FHHA‐S were immersed in anhydrous ethanol solution of FeCl 3 •6H 2 O (5 mg mL −1 ) for 24 h to obtain FHHA/Fe and FHHA‐S/Fe, respectively. The nanofibers were washed three times with anhydrous ethanol for 30 min every time to remove noncrosslinked Fe 3+ ions, and vacuum dried for 24 h to obtain the ionically crosslinked nanofibers.…”

Section: Methodsmentioning

confidence: 99%

Absorbable Thioether Grafted Hyaluronic Acid Nanofibrous Hydrogel for Synergistic Modulation of Inflammation Microenvironment to Accelerate Chronic Diabetic Wound Healing

Zhang

et al. 2020

Adv Healthcare Materials

148

103

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Current standard of care dressings are unsatisfactorily inefficacious for the treatment of chronic wounds. Chronic inflammation is the primary cause of the long‐term incurable nature of chronic wounds. Herein, an absorbable nanofibrous hydrogel is developed for synergistic modulation of the inflammation microenvironment to accelerate chronic diabetic wound healing. The electrospun thioether grafted hyaluronic acid nanofibers (FHHA‐S/Fe) are able to form a nanofibrous hydrogel in situ on the wound bed. This hydrogel degrades and is absorbed gradually within 3 days. The grafted thioethers on HHA can scavenge the reactive oxygen species quickly in the early inflammation phase to relieve the inflammation reactions. Additionally, the HHA itself is able to promote the transformation of the gathered M1 macrophages to the M2 phenotype, thus synergistically accelerating the wound healing phase transition from inflammation to proliferation and remodeling. On the chronic diabetic wound model, the average remaining wound area after FHHA‐S/Fe treatment is much smaller than both that of FHHA/Fe without grafted thioethers and the control group, especially in the early wound healing stage. Therefore, this facile dressing strategy with intrinsic dual modulation mechanisms of the wound inflammation microenvironment may act as an effective and safe treatment strategy for chronic wound management.

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“…From Figure 3A, the FTIR analysis of NFMs confirmed successful chemical crosslinking of HA by BDDE, where the peak observed at 1253 cm −1 corresponded to the newly generated C–O in ether or ester from the carboxyl groups and hydroxyl groups of HA [31]. The presence of IBU in the NFMs could be inferred from the appearance of peaks assigned to IBU in all HAIFB NFMs.…”

Section: Resultsmentioning

confidence: 90%

Ibuprofen-Loaded Hyaluronic Acid Nanofibrous Membranes for Prevention of Postoperative Tendon Adhesion through Reduction of Inflammation

Chen

Sheu

et al. 2019

IJMS

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A desirable multi-functional nanofibrous membrane (NFM) for prevention of postoperative tendon adhesion should be endowed with abilities to prevent fibroblast attachment and penetration and exert anti-inflammation effects. To meet this need, hyaluronic acid (HA)/ibuprofen (IBU) (HAI) NFMs were prepared by electrospinning, followed by dual ionic crosslinking with FeCl3 (HAIF NFMs) and covalent crosslinking with 1,4-butanediol diglycidyl ether (BDDE) to produce HAIFB NFMs. It is expected that the multi-functional NFMs will act as a physical barrier to prevent fibroblast penetration, HA will reduce fibroblast attachment and impart a lubrication effect for tendon gliding, while IBU will function as an anti-inflammation drug. For this purpose, we successfully fabricated HAIFB NFMs containing 20% (HAI20FB), 30% (HAI30FB), and 40% (HAI40FB) IBU and characterized their physico-chemical properties by scanning electron microscopy, Fourier transformed infrared spectroscopy, thermal gravimetric analysis, and mechanical testing. In vitro cell culture studies revealed that all NFMs except HAI40FB possessed excellent effects in preventing fibroblast attachment and penetration while preserving high biocompatibility without influencing cell proliferation. Although showing significant improvement in mechanical properties over other NFMs, the HAI40FB NFM exhibited cytotoxicity towards fibroblasts due to the higher percentage and concentration of IBU released form the membrane. In vivo studies in a rabbit flexor tendon rupture model demonstrated the efficacy of IBU-loaded NFMs (HAI30FB) over Seprafilm® and NFMs without IBU (HAFB) in reducing local inflammation and preventing tendon adhesion based on gross observation, histological analyses, and biomechanical functional assays. We concluded that an HAI30FB NFM will act as a multi-functional barrier membrane to prevent peritendinous adhesion after tendon surgery.

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Dual crosslinked hyaluronic acid nanofibrous membranes for prolonged prevention of post-surgical peritoneal adhesion

Abstract: A HA NFM crosslinked with FeCl3and BDDE shows prolonged degradation to prevent peritoneal adhesion.

Cited by 22 publications

References 55 publications

Synthesis and magnetic manipulation of hybrid nanobeads based on Fe3O4 nanoclusters and hyaluronic acid grafted with an ethylene glycol-based copolymer

Synthesis and magnetic manipulation of hybrid nanobeads based on Fe3O4 nanoclusters and hyaluronic acid grafted with an ethylene glycol-based copolymer

Absorbable Thioether Grafted Hyaluronic Acid Nanofibrous Hydrogel for Synergistic Modulation of Inflammation Microenvironment to Accelerate Chronic Diabetic Wound Healing

Ibuprofen-Loaded Hyaluronic Acid Nanofibrous Membranes for Prevention of Postoperative Tendon Adhesion through Reduction of Inflammation

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