Dual-color encoded DNAzyme nanostructures for multiplexed detection of intracellular metal ions in living cells

Zhou, Wenjiao; Liang, Wenbing; Li, Daxiu; Yuan, Ruo; Xiang, Yun

doi:10.1016/j.bios.2016.05.058

Cited by 82 publications

(50 citation statements)

References 50 publications

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“…For example, DNA tetrahedron nanostructures were found to readily enter mammalian cells without the use of transfection agents 156. Based on this, a DNAzyme was grafted to a DNA tetrahedron for intracellular imaging of metal ions 157. Tan and coworkers reported a DNA dendrimer scaffold that can efficiently deliver DNAzymes into cells with sufficient stability, excellent biocompatibility and retained DNAzyme catalytic activity 158.…”

Section: Delivery Of Dnazymesmentioning

confidence: 99%

Theranostic DNAzymes

Zhou¹,

Ding²,

Liu³

2017

Theranostics

203

145

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DNAzymes are catalytically active DNA molecules that are obtained via in vitro selection. RNA-cleaving DNAzymes have attracted significant attention for both therapeutic and diagnostic applications due to their excellent programmability, stability, and activity. They can be designed to cleave a specific mRNA to down-regulate gene expression. At the same time, DNAzymes can sense a broad range of analytes. By combining these two functions, theranostic DNAzymes are obtained. This review summarizes the progress of DNAzyme for theranostic applications. First, in vitro selection of DNAzymes is briefly introduced, and some representative DNAzymes related to biological applications are summarized. Then, the applications of DNAzyme for RNA cleaving are reviewed. DNAzymes have been used to cleave RNA for treating various diseases, such as viral infection, cancer, inflammation and atherosclerosis. Several formulations have entered clinical trials. Next, the use of DNAzymes for detecting metal ions, small molecules and nucleic acids related to disease diagnosis is summarized. Finally, the theranostic applications of DNAzyme are reviewed. The challenges to be addressed include poor DNAzyme activity under biological conditions, mRNA accessibility, delivery, and quantification of gene expression. Possible solutions to overcome these challenges are discussed, and future directions of the field are speculated.

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Section: Delivery Of Dnazymesmentioning

confidence: 99%

Theranostic DNAzymes

Zhou¹,

Ding²,

Liu³

2017

Theranostics

203

145

View full text Add to dashboard Cite

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“…As such, simultaneously multiplexed detection of intracellular miRNAs can be achieved. To understand the transport, distribution and regulation of metal ions at the cellular level, Xiang group proposed an attracting strategy for multiplexed sensing of intracellular UO 2 2− and Pb 2+ (Figure b) . They incorporated two metal ion‐specific DNAzymes into the DNA tetrahedron nanostructures.…”

Section: Intracellular Sensingmentioning

confidence: 99%

“…To understand the transport, distribution and regulation of metal ions at the cellular level, [80] Xiang group proposed an attracting strategy for multiplexed sensing of intracellular UO 2 2À and Pb 2 + (Figure 10b). [81] They incorporated two metal ion-specific DNAzymes into the DNA tetrahedron nanostructures. The DNAzymes activated by the target metal ions would cleave their substrates, recovering fluorescent emissions at different wavelengths.…”

Section: Intracellular Sensingmentioning

confidence: 99%

Rational Design of Framework Nucleic Acids for Bioanalytical Applications

Liu

et al. 2019

ChemPlusChem

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With the advent of DNA nanotechnology, nucleic acids have been used building blocks for constructing various DNA nanostructures. As classical and simple DNA nanostructures, framework nucleic acids (FNAs) have attracted enormous attention in the field of biosensing. The sequence‐specific self‐assembly properties and high programmability of nucleic acids allow FNAs to be incorporated in advanced probe design. In addition, FNAs enable the engineering of surfaces for biosensing (e. g., probe orientation, probe spacing), thereby improving the accessibility of target molecules to the probes arranged on heterogeneous surfaces. Moreover, FNAs offer a universal and promising platform for sensing cellular molecules because of their prominent biocompatibility and cellular permeability. Herein recent advances in FNA‐based biosensors are summarized, including electrochemical detection, optical detection, and intracellular sensing. It is hoped that this review will provide guidelines for the design and construction of FNA scaffold‐based biosensors.

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“…In this study the catalytic beacon was delivered into cells via functionalization to gold nanoparticles, and used phosphorothioate modifications on the 3′- and 5′-ends to prevent degradation by exonucleases. Subsequent studies have used cationic liposomes [49], MnO2 nanosheets [50], DNA nanostructures [51] and cell penetrating peptides [49,52] to deliver DNAzymes to cells. To increase biostability, other groups have used non-natural L-DNA, which has similar reactivity to the D-DNA enantiomer but cannot be degraded by native nucleases [53,54].…”

Section: Fluorescent Metal Ion Sensors For Environmental Monitoring Amentioning

confidence: 99%

“…Though this provides for reliable photocaging, the synthesis of the photocaged phosphoroamidite is complex, thus in 2016 Yu Xiang's lab simplified the photocaging process to a simple post-synthetic modification of a non-bridging phosphorothioate with bromo-4′ -hydroxyacetophenone on three nucleobases essential for DNAzyme activity [58]. Furthermore, Xiang et al was able to detect both UO2 2+ and Pb 2+ simultaneously in cells using DNAzyme-DNA nanostructure complexes, using another photocaging method [51]. All of these studies have enhanced our ability to probe labile metal ion distributions in cells; however, further work needs to be performed in order to use DNAzymes to accurately quantify metal ion concentrations in the cellular environment.…”

Section: Fluorescent Metal Ion Sensors For Environmental Monitoring Amentioning

confidence: 99%

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

McGhee

Loh

2017

Current Opinion in Biotechnology

125

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The on-site and real-time detection of metal ions is important for environmental monitoring and for understanding the impact of metal ions on human health. However, developing sensors selective for a wide range of metal ions that can work in the complex matrices of untreated samples and cells presents significant challenges. To meet these challenges, DNAzymes, an emerging class of metal ion-dependent enzymes selective for almost any metal ion, have been functionalized with fluorophores, nanoparticles and other imaging agents and incorporated into sensors for the detection of metal ions in environmental samples and for imaging the metal ions in living cells. Herein, we highlight the recent developments of DNAzyme-based fluorescent, colorimetric, SERS, electrochemical and electrochemiluminscent sensors for metal ions for these applications.

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Dual-color encoded DNAzyme nanostructures for multiplexed detection of intracellular metal ions in living cells

Cited by 82 publications

References 50 publications

Theranostic DNAzymes

Theranostic DNAzymes

Rational Design of Framework Nucleic Acids for Bioanalytical Applications

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

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