Dual‐Color‐Emitting Carbon Nanodots for Multicolor Bioimaging and Optogenetic Control of Ion Channels

Kim, Hyemin; Park, Yoonsang; Beack, Songeun; Han, Seulgi; Jung, Dooyup; Joon, Hyung; Kwon, Woosung; Hahn, Sei Kwang

doi:10.1002/advs.201700325

Cited by 31 publications

(24 citation statements)

References 36 publications

(30 reference statements)

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“…As shown in Figure 3 e, the emission spectra of CND1 change significantly with respect to the excitation wavelengths. This excitation dependency is frequently observed in common CNDs and is explained by the presence of various surface emissive (or defective) states [ 43 , 44 , 45 ]. In this case, the excitation dependency gradually vanished with the addition of CA.…”

Section: Resultsmentioning

confidence: 99%

Radiative and Non-Radiative Decay Pathways in Carbon Nanodots toward Bioimaging and Photodynamic Therapy

et al. 2021

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The origin and classification of energy states, as well as the electronic transitions and energy transfers associated with them, have been recognized as critical factors for understanding the optical properties of carbon nanodots (CNDs). Herein, we report the synthesis of CNDs in an optimized process that allows low-temperature carbonization using ethanolamine as the major precursor and citric acid as an additive. The results obtained herein suggest that the energy states in our CNDs can be classified into four different types based on their chemical origin: carbogenic core states, surface defective states, molecular emissive states, and non-radiative trap states. Each energy state is associated with the occurrence of different types of emissions in the visible to near-infrared (NIR) range and the generation of reactive oxygen species (ROS). The potential pathways of radiative/non-radiative transitions in CNDs have been systematically studied using visible-to-NIR emission spectroscopy and fluorescence decay measurements. Furthermore, the bright photoluminescence and ROS generation of these CNDs render them suitable for in vitro imaging and photodynamic therapy applications. We believe that these new insights into the energy states of CNDs will result in significant improvements in other applications, such as photocatalysis and optoelectronics.

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Section: Resultsmentioning

confidence: 99%

Radiative and Non-Radiative Decay Pathways in Carbon Nanodots toward Bioimaging and Photodynamic Therapy

et al. 2021

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“…Moreover, multispectral TPM can identify the composition of biological tissues labeled with several FL nanoparticles. Recently, multispectral analyses of nanoparticles using TPM have been explored for applications including therapeutic process [84,85], cell tracking optical probe [86][87][88], the morphogenetic process of neurons [89,90], and functional analysis to reveal disease progression at the cellular level [91][92][93]. FL nanoparticles also have widely been used in TPM.…”

Section: Multispectral Two-photon Microscopy Of Nanoparticlesmentioning

confidence: 99%

Review on Optical Imaging Techniques for Multispectral Analysis of Nanomaterials

Lee¹,

Kim²,

Kim³

et al. 2022

Nanotheranostics

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Biomedical imaging is an essential tool for investigating biological responses in vivo. Among the several imaging techniques, optical imaging systems with multispectral analysis of nanoparticles have been widely investigated due to their ability to distinguish the substances in biological tissues in vivo. This review article focus on multispectral optical imaging techniques that can provide molecular functional information. We summarize the basic principle of the spectral unmixing technique that enables the delineation of optical chromophores. Then, we explore the principle, typical system configuration, and biomedical applications of the representative optical imaging techniques, which are fluorescence imaging, two-photon microscopy, and photoacoustic imaging. The results in the recent studies show the great potential of the multispectral analysis techniques for monitoring responses of biological systems in vivo.

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“…[81] However, current optogenetic methods usually rely on the visible light and thus require the implantation of invasive fiber-optic probes into living tissues for signal stimulation. [82][83][84] To bypass the shallow tissue penetration issue of existing light-sensitive ion channel proteins, UCNPs with NIR light absorption but visible light emission have been applied in optogenetics.…”

Section: Optogenetics Of Neuronsmentioning

confidence: 99%

Nanotransducers for Near‐Infrared Photoregulation in Biomedicine

Duan

2019

Advanced Materials

133

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Photoregulation that utilizes light to remotely control biological events provides a precise way to decipher biology and innovate medicine; however, its potential has been limited by the shallow tissue penetration and/or phototoxicity of ultraviolet (UV)/visible light that are required to match the optical responses of endogenous photosensitive substances. Thereby, biologically friendly near-infrared (NIR) light with improved tissue penetration is desired for photoregulation. Since there are few endogenous biomolecules absorbing or emitting light in the NIR region, the development of molecular transducers is essential to convert NIR light into the cues for regulation of biological events. In this regard, optical nanomaterials able to convert NIR light into UV/visible light, heat or free radicals are competent for this task. This progress report summarizes the recent development of optical nanotransducers for NIR light-mediated photoregulation in medicine. The emerging applications including photoregulation of neural activity, gene expression, and visual systems as well as photochemical tissue bonding are highlighted along with the design principles of nanotransducers. Moreover, the current challenges and perspectives in this field are discussed.

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Dual‐Color‐Emitting Carbon Nanodots for Multicolor Bioimaging and Optogenetic Control of Ion Channels

Cited by 31 publications

References 36 publications

Radiative and Non-Radiative Decay Pathways in Carbon Nanodots toward Bioimaging and Photodynamic Therapy

Radiative and Non-Radiative Decay Pathways in Carbon Nanodots toward Bioimaging and Photodynamic Therapy

Review on Optical Imaging Techniques for Multispectral Analysis of Nanomaterials

Nanotransducers for Near‐Infrared Photoregulation in Biomedicine

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