Dual-Channel Probe of Carbon Dots Cooperating with Lanthanide Complex Employed for Simultaneously Distinguishing and Sequentially Detecting Tetracycline and Oxytetracycline

Abstract

Tetracycline (TC) and oxytetracycline (OTC) are the most widely used broad-spectrum antimicrobial agents in tetracycline drugs, and their structures and properties are very similar, so it is a great challenge to distinguish and detect these two antibiotics with a single probe at the same time. Herein, a dual-channel fluorescence probe (SiCDs@mMIPs-cit-Eu) was developed by integrating two independent reaction sites with SiCDs-doped mesoporous silica molecular imprinting group and europium complex group into a nanomaterial. The synergistic influence of inner filter effect and “antenna effect” can be guaranteed to solve the distinction between TC and OTC. Moreover, this novel strategy can also sequentially detect TC and OTC in buffer solution and real samples with high sensitivity and selectivity. This method revealed good responses to TC and OTC ranging from 0 to 5.5 μM with a detection limit of 5 and 16 nM, respectively. Combined with the smartphone color-scanning application, the portable and cheap paper-based sensor was designed to realize the multi-color visual on-site detection of TC and OTC. In addition, the logic gate device was constructed according to the fluorescence color change of the probe for TC and OTC, which provided the application possibility for the intelligent detection of the probe.

Preparation and application of carbon quantum dot fluorescent probes combined with rare earth ions

“…As shown in Fig. 5c, Xu et al developed an Eu 3+ -functionalized organo-silicon carbon dot (SiCD) dielectric molecular imprinted fluorescent probe (SiCDs@mMIPs-cit-Eu), 55 which was a dual-channel fluorescent probe that not only could simultaneously distinguish TC and OTC, but also be combined with a cellphone by preparing a paper-based sensor APP to achieve portable visual field detection. The differentiation response mechanism can be attributed to the synergistic effect of IFE and the “antenna effect”.…”

“…(b) Synthesis of CDs and fabrication of CD-Eu 3+ hybrid ratiometric fluorescence probe for the detection of OTC 54. (c) Schematic diagram of the preparation of SiCD@mMIPs-cit-Eu nanoprobe and distinction and detection of TC and OTC 55. (d) Synthetic route for CQDs and CQDs/Eu 3+ @MIP 60.…”

Dual‐emission ratiometric fluorescence sensors based on silica, molecularly imprinted polymers and carbon quantum dots for high‐sensitivity and high‐selectivity detection of sulfadiazine

“…They combine to form a core-shell system, 17 which is an innovation in single fluorescence response signal and monochromatic visual perception. 18,19 Compared with traditional fluorescence sensors, the ratiometric fluorescence sensors can more effectively resist interference, reduce errors and avoid the influence of various external factors on the test data. Because it has two fluorescence emission peaks, such a sensor can detect the content of analytes based on the fluorescence signal ratio of the two peaks.…”

“…In the design of ratiometric sensing systems, there is a single responding fluorophore with a quenching or enhancing effect and another reference fluorescence unit with constant fluorescence intensity. They combine to form a core–shell system, 17 which is an innovation in single fluorescence response signal and monochromatic visual perception 18,19 . Compared with traditional fluorescence sensors, the ratiometric fluorescence sensors can more effectively resist interference, reduce errors and avoid the influence of various external factors on the test data.…”

Gold–Silver Bimetallic Nanoclusters Protected by Glutathione S-Transferase for Colorimetric Sensing of Oxytetracycline

“…Oxytetracycline (OTC) as a common broad-spectrum tetracycline (TC) antibiotic has been widely used to fight a variety of diseases caused by bacterial infections. , However, the abuse of these antibiotics may result in bacterial drug resistance and toxic effects, which endanger human health seriously . The determination of TCs plays an important role in foodstuff and health monitoring.…”