Dual-channel imaging system for singlet oxygen and photosensitizer for PDT

Lee, Seonkyung; Isabelle, Martin; Gabally-Kinney, Kristin L.; Pogue, Brian W.; Davis, Steven J.

doi:10.1364/boe.2.001233

Cited by 32 publications

(23 citation statements)

References 22 publications

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“…35 Lee et al developed a two-channel optical system to provide spatially resolved simultaneous imaging of 1 O 2 luminescence and PS°uorescence generated during photosensitization, and the preliminary images with tumor-laden mice indicate that it is possible to obtain simultaneous images of 1 O 2 and PS distribution. 36 Most recently, we also have developed an imaging system for 1 O 2 luminescence with a cooled NIR-camera. As shown in Fig.…”

Section: -5mentioning

confidence: 99%

Singlet Oxygen Detection During Photosensitization

Lin

Chen

et al. 2013

J. Innov. Opt. Health Sci.

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Singlet oxygen ( 1 O 2 ) is a highly reactive oxygen species involved in numerous chemical and photochemical reactions in di®erent biological systems and in particular, in photodynamic therapy (PDT). However, the quanti¯cation of 1 O 2 generation during in vitro and in vivo photosensitization is still technically challenging. To address this problem, indirect and direct methods for 1 O 2 detection have been intensively studied. This review presents the available methods currently in use or under development for detecting and quantifying 1 O 2 generation during photosensitization. The advantages and limitations of each method will be presented. Moreover, the future trends in developing PDT-1 O 2 dosimetry will be brie°y discussed.

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Section: -5mentioning

confidence: 99%

Singlet Oxygen Detection During Photosensitization

Lin

Chen

et al. 2013

J. Innov. Opt. Health Sci.

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“…This effect can also occur when the photosensitizing agent is BPD, [35b] which is known to produce significant levels of 1 O 2 upon irradiation. [44] However, while autophagy appears to serve a cytoprotective function when elicited by BPD, [35b] the same effect was not observed with WST11. These results will be reported in detail elsewhere.…”

Section: Effects Of Ros Formed During Pdtmentioning

confidence: 99%

Light‐Activated Pharmaceuticals: Mechanisms and Detection

Kessel

Reiners

2012

Israel Journal of Chemistry

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Photodynamic therapy relies on the interaction between light, oxygen and a photosensitizing agent. Its medical significance relates to the ability of certain agents, usually based on porphyrin or phthalocyanine structures, to localize somewhat selectively in neoplastic cells and their vasculature. Subsequent irradiation, preferably at a sufficiently high wavelength to have a significant pathway through tissues, results in a photophysical reaction whereby the excited state of the photosensitizing agent transfers energy to molecular oxygen and results in the formation of reactive oxygen species. Analogous reactive nitrogen species are also formed. These contain both nitrogen and oxygen atoms. The net result is both direct tumor cell death and a shutdown of the tumor vasculature. Other processes may also occur that promote the anti-tumor response but these are outside the scope of this review.

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“…Several groups have demonstrated near-infrared (NIR) 1 O 2 dosimetry with a range of experimental systems. [21][22][23][24][25][26][27][28][29][30] An extensive summary of these definitive dosimetry approaches can be found in Ref. 19.…”

Section: Introductionmentioning

confidence: 99%

Multispectral singlet oxygen and photosensitizer luminescence dosimeter for continuous photodynamic therapy dose assessment during treatment

et al. 2020

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Significance: Photodynamic therapy (PDT) involves complex light-drug-pathophysiology interactions that can be affected by multiple parameters and often leads to large variations in treatment outcome from patient to patient. Direct PDT dosimetry technologies have been sought to optimize the control variables (e.g., light dose, drug administration, tissue oxygenation, and patient conditioning) for best patient outcomes. In comparison, singlet oxygen (1 O 2) dosimetry has been tested in various forms to provide an accurate and perhaps comprehensive prediction of the treatment efficacy. Aim: We discuss an advanced version of this approach provided by a noninvasive, continuous wave dosimeter that can measure near-infrared spectrally resolved luminescence of both photosensitizer (PS) and 1 O 2 generated during PDT cancer treatment. Approach: This dosimetry technology uses an amplified, high quantum efficiency InGaAs detector with spectroscopic decomposition during the light treatment to continuously extract the maximum signal of 1 O 2 phosphorescence while suppressing the strong PS luminescence background by spectrally fitting the data points across nine narrow band wavelengths. 1 O 2 and PS luminescence signals were measured in vivo in FaDu xenograft tumors grown in mice during PDT treatment using Verteporfin as the PS and a continuous laser treatment at 690 nm wavelength. Results: A cohort of 19 mice was used and observations indicate that the tumor growth rate inhibition showed a stronger correlation with 1 O 2 than with just the PS signal. Conclusions: These results suggest that 1 O 2 measurement may be a more direct dosimeter of PDT damage, and it has potential value as a definitive diagnostic for PDT treatment, especially with spectral separation of the background luminescence and online estimation of the PS concentration.

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Dual-channel imaging system for singlet oxygen and photosensitizer for PDT

Cited by 32 publications

References 22 publications

Singlet Oxygen Detection During Photosensitization

Singlet Oxygen Detection During Photosensitization

Light‐Activated Pharmaceuticals: Mechanisms and Detection

Multispectral singlet oxygen and photosensitizer luminescence dosimeter for continuous photodynamic therapy dose assessment during treatment

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