Dual-bioaugmentation strategy to enhance the formation of algal-bacteria symbiosis biofloc in aquaculture wastewater supplemented with agricultural wastes as an alternative nutrient sources and biomass support materials

Pekkoh, Jeeraporn; Chaichana, Chatchawan; Thurakit, Theera; Phinyo, Kittiya; Lomakool, Sureeporn; Ruangrit, Khomsan; Duangjan, Kritsana; Suwannarach, Nakarin; Kumla, Jaturong; Cheirsilp, Benjamas; Srinuanpan, Sirasit

doi:10.1016/j.biortech.2022.127469

Cited by 14 publications

(4 citation statements)

References 44 publications

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“…Besides, high-abundance norB and nirKS were identified in the symbiotic system microbiomes; Bacteroidota and Proteobacteria phyla increased significantly (Figure b), demonstrating the possibility of anoxic bacteria coexisting with microalgae in symbiotic systems. It is plausible that under the effect of EPS cross-linking and aggregating microalgae and bacteria (Figure S3), reactive oxygen species produced by microalgae were blocked, developing an anoxic microenvironment in the symbiotic system to assist the nitrate reduction process of denitrifying bacteria. , …”

Section: Resultsmentioning

confidence: 99%

“…It is plausible that under the effect of EPS cross-linking and aggregating microalgae and bacteria (Figure S3), reactive oxygen species produced by microalgae were blocked, developing an anoxic microenvironment in the symbiotic system to assist the nitrate reduction process of denitrifying bacteria. 35,36 It is noteworthy that N assimilation was enhanced in the symbiotic system (Figure 2a), which may be related to the existence of growth-promoting bacteria (Allorhizobium sp., Rhizobium, etc.) that supported the growth of assimilating microalgae.…”

Section: Mechanisms Of Strategies Formentioning

confidence: 98%

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Bidirectional Enhancement of Nitrogen Removal by Indigenous Synergetic Microalgal–Bacterial Consortia in Harsh Low-C/N Wastewater

Geng,

Xiong,

Yang

et al. 2024

Environ. Sci. Technol.

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Conventional microalgal−bacterial consortia have limited capacity to treat low-C/N wastewater due to carbon limitation and single nitrogen (N) removal mode. In this work, indigenous synergetic microalgal−bacterial consortia with high N removal performance and bidirectional interaction were successful in treating rare earth tailing wastewaters with low-C/N. Ammonia removal reached 0.89 mg N L −1 h −1 , 1.84-fold more efficient than a common microalgal−bacterial system. Metagenomics-based metabolic reconstruction revealed bidirectional microalgal−bacterial interactions. The presence of microalgae increased the abundance of bacterial N-related genes by 1.5-to 57-fold. Similarly, the presence of bacteria increased the abundance of microalgal N assimilation by 2.5-to 15.8-fold. Furthermore, nine bacterial species were isolated, and the bidirectional promotion of N removal by the microalgal−bacterial system was verified. The mechanism of microalgal N assimilation enhanced by indole-3-acetic acid was revealed. In addition, the bidirectional mode of the system ensured the scavenging of toxic byproducts from nitrate metabolism to maintain the stability of the system. Collectively, the bidirectional enhancement system of synergetic microalgae-bacteria was established as an effective N removal strategy to broaden the stable application of this system for the effective treatment of low C/N ratio wastewater.

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Section: Resultsmentioning

confidence: 99%

Section: Mechanisms Of Strategies Formentioning

confidence: 98%

Bidirectional Enhancement of Nitrogen Removal by Indigenous Synergetic Microalgal–Bacterial Consortia in Harsh Low-C/N Wastewater

Geng,

Xiong,

Yang

et al. 2024

Environ. Sci. Technol.

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“…Furthermore, these carbon sources have provided a favorable culture environment that might include rotifers, feeds, or other facilities. For example, Pekkoh et al [60] stated that the addition of MO and RB mixture in a BFT system had ), (d) ammonia-nitrogen (mg L −1 ), (e) nitrite-nitrogen (mg L −1 ), and (f ) nitrate-nitrogen (mg L −1 ) in control and four different carbon sources using biofloc systems for the culture of Brachionus plicatilis. CON: control; MO: molasses; RB: rice bran; MS: maize starch; and PKE: palm kernel expeller.…”

Section: Discussionmentioning

confidence: 99%

Effects of Different Carbon Sources on the Growth and Production of Rotifer (Brachionus plicatilis) in a Zero-Water Exchange Biofloc Culture System

Hosain,

Amin,

Kamarudin

et al. 2024

Aquaculture Research

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Brachionus plicatilis is considered an indispensable first live feed for many fish and crustacean larvae; the demand for the species has increased globally. The mass production of the rotifer involves quality microalga and a standard diet; this culture is expensive and needs a skilled workforce. The hatchery’s incubators are likely to have limited resources leading to sudden rotifer culture crashes that ultimately disrupt the larvae production. More recently, improved sustainable rotifer production has been achieved through biofloc technology (BFT) that uses fish wastes and wheat flour. However, various carbon sources, which are typically used in BFT-based systems need to be explored and tested for their efficacies. A 4-day rotifer, B. plicatilis batch culture, was conducted in BFT systems by adding four carbon sources: molasses, rice bran, maize starch, and palm kernel expeller versus a control (without any carbon source). Fifteen 125 L containing polyethylene tanks with a water volume of 100 L were used for this experiment, and each tank was stocked with 5 × 106 rotifer (50 rotifers mL−1). Different carbon sources in triplicates including a control were tested as treatments. The carbon : nitrogen ratio in the study was maintained at 10 : 1. The rotifers were fed with Baker’s yeast at 1.0, 0.50, and 0.25 g million-−1 rotifers for the first, second, and third day and continued after that. Total ammonia–nitrogen (TAN) and pH values were found to be significantly (p<0.05) lower in all four treatments of the BFT system than in the control. Significantly higher (p<0.05) settleable solids were obtained in the molasses and rice bran treatments than those in the maize starch or palm kernel expeller. Likewise, the significantly (p<0.05) higher density of B. plicatilis and their specific growth rate were obtained in the molasses and rice bran-adding treatments, followed by those in palm kernel expeller, maize starch, and the control. This study indicates that molasses and rice bran as carbon sources when added to BFT-based systems enhance B. plicatilis production.

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“…In a study using an aquaculture WW treatment, an inoculum consisting of algae and bacteria was tested. This consortium provided the most compact biofloc structure (0.59 g/L), high settleability (71.91%), and a large particle diameter (4.25 mm) [ 30 ]. The mixed salt-tolerant bacteria system composed of ammonia-, nitrite-, and nitrate–nitrogen-utilising bacteria was artificially constructed for the salt-tolerant aerobic granular sludge.…”

Section: Obtaining Microorganism Degraders: Sources and Methodsmentioning

confidence: 99%

Current Trends in Bioaugmentation Tools for Bioremediation: A Critical Review of Advances and Knowledge Gaps

Muter

2023

Microorganisms

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Bioaugmentation is widely used in soil bioremediation, wastewater treatment, and air biofiltration. The addition of microbial biomass to contaminated areas can considerably improve their biodegradation performance. Nevertheless, analyses of large data sets on the topic available in literature do not provide a comprehensive view of the mechanisms responsible for inoculum-assisted stimulation. On the one hand, there is no universal mechanism of bioaugmentation for a broad spectrum of environmental conditions, contaminants, and technology operation concepts. On the other hand, further analyses of bioaugmentation outcomes under laboratory conditions and in the field will strengthen the theoretical basis for a better prediction of bioremediation processes under certain conditions. This review focuses on the following aspects: (i) choosing the source of microorganisms and the isolation procedure; (ii) preparation of the inoculum, e.g., cultivation of single strains or consortia, adaptation; (iii) application of immobilised cells; (iv) application schemes for soil, water bodies, bioreactors, and hydroponics; and (v) microbial succession and biodiversity. Reviews of recent scientific papers dating mostly from 2022–2023, as well as our own long-term studies, are provided here.

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Dual-bioaugmentation strategy to enhance the formation of algal-bacteria symbiosis biofloc in aquaculture wastewater supplemented with agricultural wastes as an alternative nutrient sources and biomass support materials

Cited by 14 publications

References 44 publications

Bidirectional Enhancement of Nitrogen Removal by Indigenous Synergetic Microalgal–Bacterial Consortia in Harsh Low-C/N Wastewater

Bidirectional Enhancement of Nitrogen Removal by Indigenous Synergetic Microalgal–Bacterial Consortia in Harsh Low-C/N Wastewater

Effects of Different Carbon Sources on the Growth and Production of Rotifer (Brachionus plicatilis) in a Zero-Water Exchange Biofloc Culture System

Current Trends in Bioaugmentation Tools for Bioremediation: A Critical Review of Advances and Knowledge Gaps

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