Dual and divergent transcriptional impact of IS1548 insertion upstream of the peptidoglycan biosynthesis gene murB of Streptococcus agalactiae

Khazaal, Sarah; Safadi, Rim Al; Osman, Dani; Hiron, Aurélia; Gilot, Philippe

doi:10.1016/j.gene.2019.144094

Cited by 4 publications

(15 citation statements)

References 36 publications

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“…We previously identified σ 70 transcriptional promoters inside the folK gene (P 1 : −10 box, TTGAATTAT; −35 box, TAGAGA) and in the folK–murB intergenic region (P murB : −10 box, TGGTATAAT; −35 box, TCGTCA). Both of them were shown to be involved in the transcription of murB [ 40 ]. We here also identified putative σ 70 transcriptional promoters in the potD–clc intergenic region (P clc ; −10 box: TGTTAAAAT, −35 box: TTCCTA).…”

Section: Resultsmentioning

confidence: 99%

“…2 , lane 8b). We previously showed that folK is co-transcribed with murB in strain SA87 [ 40 ]. By using the SK17 reverse primer annealing in murB and the SK16 forward primer annealing in folK ( Table 2 , Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Standard nucleic acid manipulation techniques were carried out as described previously [ 45 ]. S. agalactiae genomic DNA and RNA purifications were performed as previously described [ 40 ]. Plasmids were purified from E. coli with a NucleoSpin Plasmid kit (Macherey‐Nagel), according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…The presence of this insertion sequence prevents the co-transcription of murB with genes of the folate pathway. However, as IS 1548 brings an additional promoter able to initiate murB transcription, the insertion of IS 1548 results in a minor negative modulation of the expression of murB [ 40 ]. In S. pneumoniae and S. suis, it was demonstrated that murB is co-transcribed with potABCD , which suggests also a relationship between polyamine transport and peptidoglycan biosynthesis in streptococci [ 19, 41 ].…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the polyamine biosynthetic and transport capability of Streptococcus agalactiae: the non-essential PotABCD transporter

et al. 2021

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Polyamines constitute a group of organic polycations positively charged at physiological pH. They are involved in a large variety of biological processes, including the protection against physiological stress. In this study, we show that the genome of Streptococcus agalactiae , a commensal bacterium of the intestine and the vagina and one of the most common agents responsible of neonate infections, does not encode proteins homologous to the specific enzymes involved in the known polyamine synthetic pathways. This lack of biosynthetic capability was verified experimentally by TLC analysis of the intracellular content of S. agalactiae grown in the absence of polyamines. However, similar analyses showed that the polyamines spermidine, spermine and putrescine can be imported from the growth media into the bacteria. We found that all strains of S. agalactiae possess the genes encoding the polyamine ABC transporter PotABCD. We demonstrated that these genes form an operon with folK, a gene involved in folate biosynthesis, murB, a gene involved in peptidoglycan biosynthesis, and with clc, a gene encoding a Cl−/H+ antiporter involved in resistance to acid stress in Escherichia coli . Transcription of the potABCD operon is induced by peroxide-induced oxidative stress but not by acidic stress. Spermidine and spermine were found to be inducers of potABCD transcription at pH 7.4 whereas putrescine induces this expression only during peroxide-induced oxidative stress. Using a deletion mutant of potABCD, we were nevertheless unable to associate phenotypic traits to the PotABCD transporter, probably due to the existence of one or more as yet identified transporters with a redundant action.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigation of the polyamine biosynthetic and transport capability of Streptococcus agalactiae: the non-essential PotABCD transporter

et al. 2021

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“…Insertion sequences (ISs) are a kind of intracellular MGEs or transposable elements that have an important impact on genome structure and function [47]. Various ISs have been reported in GBS strains [48][49][50], some of which cause genetic alterations or frameshift mutations that change the virulence or invasiveness of the bacteria. To our knowledge, this is the first study to find a significant relationship of negative ISSag5 and loss of PI-1.…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Serotype III/ST-17 Group B Streptococcus Strains with Antimicrobial Resistance Using Whole Genome Sequencing

et al. 2021

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Background: Antibiotic-resistant type III/ST-17 Streptococcus agalactiae (group B Streptococcus, GBS) strain is predominant in neonatal invasive GBS diseases. We aimed to investigate the antibiotic resistance profiles and genetic characteristics of type III/ST-17 GBS strains. Methods: A total of 681 non-duplicate GBS isolates were typed (MLST, capsular types) and their antibiotic resistances were performed. Several molecular methods (WGS, PCR, sequencing and sequence analysis) were used to determine the genetic context of antibiotic resistant genes and pili genes. Results: The antibiotic resistant rates were significantly higher in type Ib (90.1%) and type III (71.1%) GBS isolates. WGS revealed that the loss of PI-1 genes and absence of ISSag5 was found in antibiotic-resistant III/ST-17 GBS isolates, which is replaced by a ~75-kb integrative and conjugative element, ICESag37, comprising multiple antibiotic resistance and virulence genes. Among 190 serotype III GBS isolates, the most common pilus island was PI-2b (58.4%) alone, which was found in 81.3% of the III/ST-17 GBS isolates. Loss of PI-1 and ISSag5 was significantly associated with antibiotic resistance (95.5% vs. 27.8%, p < 0.001). The presence of ICESag37 was found in 83.6% of all III/ST-17 GBS isolates and 99.1% (105/106) of the antibiotic-resistant III/ST-17 GBS isolates. Conclusions: Loss of PI-1 and ISSag5, which is replaced by ICESag37 carrying multiple antibiotic resistance genes, accounts for the high antibiotic resistance rate in III/ST-17 GBS isolates. The emerging clonal expansion of this hypervirulent strain with antibiotic resistance after acquisition of ICESag37 highlights the urgent need for continuous surveillance of GBS infections.

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Data on the link between genomic integration of IS1548 and lineage of the strain obtained by bioinformatic analyses of sequenced genomes of Streptococcus agalactiae available at the National Center for Biotechnology Information database

et al. 2020

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Dual and divergent transcriptional impact of IS1548 insertion upstream of the peptidoglycan biosynthesis gene murB of Streptococcus agalactiae

Cited by 4 publications

References 36 publications

Investigation of the polyamine biosynthetic and transport capability of Streptococcus agalactiae: the non-essential PotABCD transporter

Investigation of the polyamine biosynthetic and transport capability of Streptococcus agalactiae: the non-essential PotABCD transporter

Genomic Characterization of Serotype III/ST-17 Group B Streptococcus Strains with Antimicrobial Resistance Using Whole Genome Sequencing

Data on the link between genomic integration of IS1548 and lineage of the strain obtained by bioinformatic analyses of sequenced genomes of Streptococcus agalactiae available at the National Center for Biotechnology Information database

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