Dual agents loaded PLGA nanoparticles: Systematic study of particle size and drug entrapment efficiency

Song, Xiangrong; Yu, Zhao; Hou, Shixiang; Xu, Fangyuan; Zhao, Ruifeng; He, Jiangyan; Cai, Zheng; Li, Yuanbo; Chen, Qiuhong

doi:10.1016/j.ejpb.2008.01.013

Cited by 255 publications

(200 citation statements)

References 31 publications

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“…Nevertheless, the preparation of nanocapsules with concentrations of PVA higher than 1%, induced an increasing of the resulting size and polydispersity. This finding would be also an evidence that with the increasing in PVA concentration, the viscosity of the outer aqueous phase increased in the same way, which resulted in size increase due to decrease in the net shear stress [31,32] Figure 1: Influence of the PVA concentration on the average hydrodynamic size and PDI of the resulting MagPCL-NC. Data expressed as mean ± SD (n = 3).…”

mentioning

confidence: 79%

“…This fact would be consequence of an increase in both the viscosity and the surface tension of the organic phase in which PCL was dissolved and, hence reducing the net shear stress and promoting formation of droplets with a large size [32]. Furthermore, PVA was probably insufficient to cover the surface of droplets completely when the amount of PCL is higher, which gives the coalescence of droplets in the process of evaporation of organic solvent and resulting in aggregation of nanocapsules after the removal of organic solvent [32]. This behavior is in agreement with previously published reports for polymeric nanocapsules [33][34][35].…”

Section: Pcl Concentrationmentioning

confidence: 99%

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Superparamagnetic maghemite loaded poly (ε- caprolactone) nanocapsules : characterization and synthesis optimization

et al. 2014

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Iron oxide nanoparticles (ION) have been studied for essential applications, like detection of biological constituents (virus, bacterials, cell, nucleic acids, protein, enzyme, etc.), magnetic bioseparation and clinic therapy and diagnosis (such as MRI magnetic fluid and hyperthermia). In this work, γ-Fe 2 O 3 has been synthetized by a adapted sol-gel method and entraped in poly ε-caprolactone (PCL) nanocapsules. The superparamagnetic nanocapsules have been formulated by double emulsion evaporation method. Some variables affecting the polydispersity index, zeta potential surface and size of nanocapsules were studied aiming optimize the formulation process of maghemite-loaded PCL nanocapsules. The following parameters were selected: sonication time, PCL concentration in organic phase, PVA concentration in external aqueous phase and maghemite/PCL weight ratio. Under these experimental conditions, the resulting nanocapsules displayed a mean size of about 346 nm and a maghemite content of about 7.5 µg/mg of nanocapsules and superparamagnetic behaviour at room temperature.

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mentioning

confidence: 79%

Section: Pcl Concentrationmentioning

confidence: 99%

Superparamagnetic maghemite loaded poly (ε- caprolactone) nanocapsules : characterization and synthesis optimization

et al. 2014

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“…However, with the increase in PVA concentration, the viscosity of the external aqueous phase increased in the same way, which resulted in size increase due to decrease in the net shear stress. 34,35 At a high PVA concentration (2%), more molecules of PVA may be oriented in organic solvent/water interface to reduce efficiently the interfacial tension which resulted in significant increase in the net shear stress at a constant energy density during emulsification and promoted the formation of smaller emulsion droplets PDI values lower than 0.3 are satisfactory and zeta potential variation in the range of -3 and -8…”

Section: Pva Concentration In the Aqueous Phasementioning

confidence: 99%

Optimization of maghemite-loaded PLGA nanospheres for biomedical applications

Silva

Hechenleitner

Oliveira

et al. 2013

European Journal of Pharmaceutical Sciences

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“…the plasmid loaded plgA-nps (pDnA-PLGA-NPs) were prepared by a modified method of w/o/w double-emulsion process of our lab (20,21). the encapsulating efficiency of PLGA-NPs for pDNA was determined by the ratio of pDnA incorporated in plgA-nps to the total amount of pDnA in formulation (22). particle size and zeta potential of pDnA-plgA-nps were determined using zetasizer nano zs90 (Malvern Instrument, UK).…”

Section: Methodsmentioning

confidence: 99%

Efficient inhibition of ovarian cancer by short hairpin RNA targeting claudin-3

Sun

Yi²,

Song³

et al. 2011

Oncol Rep

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Abstract. ovarian cancer is one of the most lethal gynecologic neoplasms. even though various new chemotherapeutics have been developed for the treatment of ovarian cancer, drug resistance and undesired serious side effects remain unavoidable obstacles for chemotherapeutic approaches. new strategies to overcome the therapeutic dilemma are needed. claudin-3 (clDn3) is a recently discovered gene generally overexpressed in human ovarian cancers but not in normal ovarian tissue. Its high expression has been identified to associate with the invasion, proliferation and survival of cancer cells, making it a promising target for gene therapy of ovarian cancer. However, in gene therapy, traditional gene carriers such as virus or cationic liposomes suffer from distressing shortcomings of potential carcinogenicity, obvious cytotoxicity and immunogenicity. nanoparticles (nps) based on plgA are a novel gene delivery system with good biodegradability, excellent biocompatibility and low toxcity for in vivo gene delivery compared with traditional gene carriers. We constructed a plasmid expressing shrnA targeted clDn3 (pshclDn3) encapsulated with plgA-nps, and administered it by i.p. injection to nude mice bearing intraperitoneal sKoV3 ovarian cancer, to investigate the antitumor potential of knocking down clDn3. After 12 times of administration, the tumors of each group were compared. the underlying antitumor mechanisms were revealed by immunostaining of cD31, Ki-67 and tUnel assay, to exhibit possible alterations in microvessel density, cell proliferation and cell apoptosis. our study demonstrated that i.p. administration of pshclDn3 effectively suppressed the expression of clDn3 and, thus, inhibited the growth of ovarian tumors, significantly reducing tumor weight by 67.4% compared with blank controls (p<0.05). Immunostaining of cD31, Ki-67 and tUnel assay demonstrated decreased angiogenesis (p<0.05), reduced proliferation (p<0.05) and increased apoptosis (p<0.05) in the pshclDn3 treated group compared with controls. no obvious toxicity of plgA-nps was observed either in vitro or in vivo. our results indicated that knockdown of clDn3 by pshclDn3 encapsulated in plgA nps may provide a promising approach for the treatment of ovarian cancer. IntroductionOvarian cancer, which accounts for more than 50% death of gynecologic malignancy, is the most lethal gynecologic carcinoma (1). Due to its few and imperceptible early symptoms, ovarian cancer is mostly at advanced stage in most patients during the initial diagnosis. Although patients may initially respond to the combination treatment of surgical and chemical therapies, most of them will inevitably die from relapse and the development of chemotherapy-resistant recurrence (2). The overall 5-year relative survival rate is no more than 50% (3). therefore, new strategies to improve the treatment status of ovarian cancer are urgently needed.gene therapy is a novel therapeutic strategy different from traditional therapies. It exclusively targets the key gene for tumorigenesis, achieves ...

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Dual agents loaded PLGA nanoparticles: Systematic study of particle size and drug entrapment efficiency

Cited by 255 publications

References 31 publications

Superparamagnetic maghemite loaded poly (ε- caprolactone) nanocapsules : characterization and synthesis optimization

Superparamagnetic maghemite loaded poly (ε- caprolactone) nanocapsules : characterization and synthesis optimization

Optimization of maghemite-loaded PLGA nanospheres for biomedical applications

Efficient inhibition of ovarian cancer by short hairpin RNA targeting claudin-3

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