Dual Activities of Oxidation and Oxidative Decarboxylation by Flavoenzymes

Trisrivirat, Duangthip; Sutthaphirom, Chalermroj; Pimviriyakul, Panu; Chaiyen, Pimchai

doi:10.1002/cbic.202100666

Cited by 4 publications

(2 citation statements)

References 85 publications

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“…Only cytochrome oxidase, which has the greatest affinity for oxygen, is capable of transferring electrons directly to oxygen. Cytochrome oxidase inhibitors are CO, cyanide, and azide [15].…”

Section: Mitochondrial Sequestration Of Ca 2 +mentioning

confidence: 99%

Mechanisms of Energy Transduction in Mitochondria

2023

CTBEB

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Mitochondria play a key role in providing energy for cellular functions, and on the other hand, they are a target, decoder and switch of intracellular signals, generator of secondary messengers and pro-apoptotic factors. Modern ideas about the mechanisms of energy transduction in the mitochondrial membrane were formed after Peter Mitchell created the chemiosmotic theory of ATP synthesis. The data presented in the article can serve as a fundamental basis for further study of the brain in normal and pathological conditions with further extrapolation of the obtained data to humans. Mitochondria play a key role in providing energy for cellular functions, and on the other hand, they are a target, decoder and switch of intracellular signals, generator of secondary messengers and pro-apoptotic factors [1]. Modern ideas about the mechanisms of energy transduction in the mitochondrial membrane were formed after Peter Mitchell created the chemiosmotic theory of ATP synthesis. The energy resources used by the inner mitochondrial membrane are initially directed at the transport of the conjugating proton through the membrane to an area with a higher concentration (energization of the membrane), then the proton gradient is used by the mitochondria as a driving force capable of leading to the synthesis of ATP. Isolation of mitochondrial proteins by D.E. Green and co-workers led to the formation of the idea of “complexes” of the respiratory chain [2].

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Section: Mitochondrial Sequestration Of Ca 2 +mentioning

confidence: 99%

Mechanisms of Energy Transduction in Mitochondria

2023

CTBEB

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“…Inexpensive tyrosine (6) and dopamine (8) were used as the starting materials to prepare (S)-norcoclaurine using the green Pictet-Spengler reaction. First, tyrosine underwent oxidative decarboxylation to produce 4-hydroxyphenylacetadehyde (7) [56]. Then, dopamine and NCS with ascorbate were added to avoid oxidation and to afford the desired (S)-norcoclaurine using a one-pot, two-step process.…”

Section: Enzymatic and Chemoenzymatic Approaches For Alkaloidsmentioning

confidence: 99%

Recent Advances in the Synthesis of Marine-Derived Alkaloids via Enzymatic Reactions

et al. 2022

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Alkaloids are a large and structurally diverse group of marine-derived natural products. Most marine-derived alkaloids are biologically active and show promising applications in modern (agro)chemical, pharmaceutical, and fine chemical industries. Different approaches have been established to access these marine-derived alkaloids. Among these employed methods, biotechnological approaches, namely, (chemo)enzymatic synthesis, have significant potential for playing a central role in alkaloid production on an industrial scale. In this review, we discuss research progress on marine-derived alkaloid synthesis via enzymatic reactions and note the advantages and disadvantages of their applications for industrial production, as well as green chemistry for marine natural product research.

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N-Formimidoylation/-iminoacetylation modification in aminoglycosides requires FAD-dependent and ligand-protein NOS bridge dual chemistry

et al. 2023

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Oxidized cysteine residues are highly reactive and can form functional covalent conjugates, of which the allosteric redox switch formed by the lysine-cysteine NOS bridge is an example. Here, we report a noncanonical FAD-dependent enzyme Orf1 that adds a glycine-derived N-formimidoyl group to glycinothricin to form the antibiotic BD-12. X-ray crystallography was used to investigate this complex enzymatic process, which showed Orf1 has two substrate-binding sites that sit 13.5 Å apart unlike canonical FAD-dependent oxidoreductases. One site could accommodate glycine and the other glycinothricin or glycylthricin. Moreover, an intermediate-enzyme adduct with a NOS-covalent linkage was observed in the later site, where it acts as a two-scissile-bond linkage facilitating nucleophilic addition and cofactor-free decarboxylation. The chain length of nucleophilic acceptors vies with bond cleavage sites at either N–O or O–S accounting for N-formimidoylation or N-iminoacetylation. The resultant product is no longer sensitive to aminoglycoside-modifying enzymes, a strategy that antibiotic-producing species employ to counter drug resistance in competing species.

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Dual Activities of Oxidation and Oxidative Decarboxylation by Flavoenzymes

Cited by 4 publications

References 85 publications

Mechanisms of Energy Transduction in Mitochondria

Mechanisms of Energy Transduction in Mitochondria

Recent Advances in the Synthesis of Marine-Derived Alkaloids via Enzymatic Reactions

N-Formimidoylation/-iminoacetylation modification in aminoglycosides requires FAD-dependent and ligand-protein NOS bridge dual chemistry

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