Du choix et de la préparation des réactifs en vue de la détection de l'antigène Australia chez les donneurs de sang

North, M. L.; Schmitthaeusler, R.; Ernewein, L.; Kandel, G.; Malgras, J; Waitz, R

doi:10.1016/s0035-2977(72)80036-1

“…Tow techniques were employed: CEP [17] and RID in the liquid phase [1][2][3]. The HBs antigen is sought at each fractionation stage by these two methods, using samples in which the protein concentration is brought down to an identical value, close to that of a normal plasma.…”

Section: Detection Of the Hbs Antigenmentioning

confidence: 99%

HBs Antigen in Human Plasma Fractions

Leveque¹,

Drouet²,

et al. 1975

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Therapeutic fractions were obtained by fractionating human plasma containing HBs antigen, by the Cohn ethanol technique modified by Nitschmann. Plasmas with various HBs antigen titers were selected. The antigen was sought in each fraction by the techniques of counterelectrophoresis and radioimmunological detection in liquid phase. With the exception of very pure albumins, all fractions were found to contain the HBs antigen, though in variable proportions. A technique for the purification of albumin, applicable on an industrial scale, is proposed. It makes it possible to obtain an albumin fraction in which the HBs antigen cannot be detected by the most sensitive techniques, even starting with a plasma which is very rich in HBs antigen (titer 1/64 by counterelectro phoresis).

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La géographie de l'antigène Australia chez les donneurs de sang de la France métropolitaine

1972

Revue Française de Transfusion

2

0

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HBs Antigen in Human Plasma Fractions

Leveque

¹

,

Drouet

²

,

Schmitthaeusler

³

et al. 1975

View full text Add to dashboard Cite

Therapeutic fractions were obtained by fractionating human plasma containing HBs antigen, by the Cohn ethanol technique modified by Nitschmann. Plasmas with various HBs antigen titers were selected. The antigen was sought in each fraction by the techniques of counterelectrophoresis and radioimmunological detection in liquid phase. With the exception of very pure albumins, all fractions were found to contain the HBs antigen, though in variable proportions. A technique for the purification of albumin, applicable on an industrial scale, is proposed. It makes it possible to obtain an albumin fraction in which the HBs antigen cannot be detected by the most sensitive techniques, even starting with a plasma which is very rich in HBs antigen (titer 1/64 by counterelectrophoresis).

show abstract

Du choix et de la préparation des réactifs en vue de la détection de l'antigène Australia chez les donneurs de sang

Cited by 3 publications

References 5 publications

HBs Antigen in Human Plasma Fractions

HBs Antigen in Human Plasma Fractions

La géographie de l'antigène Australia chez les donneurs de sang de la France métropolitaine

HBs Antigen in Human Plasma Fractions

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