DT-Diaphorase Induction by Lead Acetate in the Liver of Rats

Arizono, Koji; Sugiura, Shigeki; Miyazato, Shoko; Takiguchi, Mitsuyoshi; Ariyoshi, Toshihiko

doi:10.1007/s001289900153

Cited by 3 publications

(2 citation statements)

References 12 publications

(7 reference statements)

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“…This NQO1-mediated reduction mechanism may be responsible for scavenging superoxides anions generated during oxidative stress as a cellular defense against various toxic quinones. Significant expressions of NQO1 have been reported in response to liver damage or primary biliary cirrhosis in human liver (27) or rat liver (28), and NQO1 expression is altered in a plethora of cancers cells (29). Also, NQO1 plays a key role in regulating the p53 tumor suppressor gene, a labile protein that induces either growth arrest or controlled apoptosis (30,31).…”

Section: Discussionmentioning

confidence: 99%

In vivo MRSI of hyperpolarized [1‐¹³C]pyruvate metabolism in rat hepatocellular carcinoma

et al. 2010

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Hepatocellular carcinoma (HCC), the primary form of human adult liver malignancy, is a highly aggressive tumor with average survival rates that are currently less than a year following diagnosis. Most patients with HCC are diagnosed at an advanced stage, and no efficient marker exists for predicting prognosis and/or response(s) to therapy. We previously reported a high level of [1-13 C] alanine in an orthotopic HCC using single-voxel hyperpolarized [1-13 C] pyruvate magnetic resonance spectroscopy (MRS). In the present study, we implemented a 3D-magnetic resonance spectroscopic imaging (MRSI) sequence to investigate this potential hallmark of cellular metabolism in rat livers bearing HCC (N=7 buffalo rats). In addition, quantitative realtime PCR was used to determine the mRNA levels of lactate dehydrogenase A, NAD(P)H dehydrogenase quinone 1, and alanine transaminase. The enzymes levels were significantly higher in the tumor than the normal liver tissues within each rat, which is associated with the in vivo MRSI signal of [1-13 C]alanine and [1-13 C]lactate after a bolus intravenous injection of [1-13 C]pyruvate. Histopathological analysis of these tumors confirms successful growth of HCC as a nodule in buffalo rats' livers revealing malignancy and hyper-vascular architecture. More importantly, the results demonstrate that the metabolic fate of [1-13 C]pyruvate conversion to [1-13 C]alanine significantly supersedes that of [1-13 C]pyruvate conversion to [1-13 C]lactate potentially serving as a marker of HCC tumors.

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Section: Discussionmentioning

confidence: 99%

In vivo MRSI of hyperpolarized [1‐¹³C]pyruvate metabolism in rat hepatocellular carcinoma

et al. 2010

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“…The absorbed lead is conjugated in the liver (Grandjean, 1979;Fowler et al, 1980 ), leading to impaired liver functions ( Valle and Ulmer, 1972;Singhal et al, 1973;Thrivikraman et al, 1978;Flora et al, 1991;Rijhsinghani et al, 1993;Sherlock and Dooley, 1993;Sandhir and Gill, 1995;Shinozuka et al, 1996 ). Considerable literature have accumulated on the biochemical activities of hepatic enzymes during lead intoxication ( Zook et al, 1972;Singhal et al, 1973;Kowalczyk, 1976Kowalczyk, , 1986Fowler et al, 1980;Flora et al, 1991;Morgan et al, 1991;Fantin et al, 1992;Tsafaris and Alexaki, 1992;Khan et al, 1993;Nehru and Kaushal, 1993;Singh et al, 1994;Sandhir and Gill, 1995;Arizono et al, 1996) , while very limited histochemical studies, if any, were carried out on the effects of chronic subtoxic doses of lead on hepatic enzymes.…”

Section: Introductionmentioning

confidence: 99%

Histochemical demonstration of changes in the activity of hepatic phosphatases induced by experimental lead poisoning in male white rats (Rattus norvegicus)

Jarrar

Mahmoud

2000

Toxicol Ind Health

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Seven hepatic phosphatases were histochemically investigated in male white rats (Rattus norvegicus) pretreated with chronic subtoxic doses of lead acetate. Lead has increased the activities of alkaline-, acid-, neutral-, adenosine mono- and glucose-6-phosphatase, but has markedly decreased the activity of membrane-bound Na+-K+, ATPase while the activity of mitochondrial Mg2+-ATPase was not altered. It has also produced heterogenous alterations in the distribution patterns, sites of the enzymatic activities and in the intensity of phosphatase activities among the same type of cells in the terminal afferent and efferent venules of the hepatic lobules. The obtained histochemical findings indicate that the alterations in the activities of hepatic phosphatases could be an adaptation to the metabolic, structural and functional changes in the organelles of hepatic cells due to lead intoxication.

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Comparison of key enzymes in the zebra mussel, Dreissena polymorpha, the earthworm Allolobophora chlorotica and Chironomus riparius larvae

Osman

Noort

2007

Ecotoxicology and Environmental Safety

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DT-Diaphorase Induction by Lead Acetate in the Liver of Rats

Cited by 3 publications

References 12 publications

In vivo MRSI of hyperpolarized [1‐¹³C]pyruvate metabolism in rat hepatocellular carcinoma

In vivo MRSI of hyperpolarized [1‐¹³C]pyruvate metabolism in rat hepatocellular carcinoma

Histochemical demonstration of changes in the activity of hepatic phosphatases induced by experimental lead poisoning in male white rats (Rattus norvegicus)

Comparison of key enzymes in the zebra mussel, Dreissena polymorpha, the earthworm Allolobophora chlorotica and Chironomus riparius larvae

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DT-Diaphorase Induction by Lead Acetate in the Liver of Rats

Cited by 3 publications

References 12 publications

In vivo MRSI of hyperpolarized [1‐13C]pyruvate metabolism in rat hepatocellular carcinoma

In vivo MRSI of hyperpolarized [1‐13C]pyruvate metabolism in rat hepatocellular carcinoma

Histochemical demonstration of changes in the activity of hepatic phosphatases induced by experimental lead poisoning in male white rats (Rattus norvegicus)

Comparison of key enzymes in the zebra mussel, Dreissena polymorpha, the earthworm Allolobophora chlorotica and Chironomus riparius larvae

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In vivo MRSI of hyperpolarized [1‐¹³C]pyruvate metabolism in rat hepatocellular carcinoma

In vivo MRSI of hyperpolarized [1‐¹³C]pyruvate metabolism in rat hepatocellular carcinoma