DsRNA-mediated protection against two isolates of Papaya ringspot virus through topical application of dsRNA in papaya

Tharanath, Vadlamudi; Patil, Basavaprabhu L.; Kaldis, Athanasios; Gopal, D. V. R. Sai; Mishra, Ritesh; Berbati, Margarita; Voloudakis, Andreas E.

doi:10.1016/j.jviromet.2019.113750

Cited by 35 publications

(17 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We chose the CP coding region for ToMV, and for experiments with PVY. Actually, the cp gene has been the preferred to induce viral resistance by dsRNA topical application 14,15,21,[23][24][25][26] . Pooggin 39 proposed that the viral genomic regions that do not generate a large quantity of siRNA molecules in virus-infected plants are more promising targets for protection than viral siRNA hotspot regions.…”

Section: Discussionmentioning

confidence: 99%

“…The dsRNA of the cp gene was selected for the further experiments with ToMV in tomato plants as it induced a slightly lower average number of lesions in both hypersensitive hosts ( Supplementary Fig. S1), and because it is commonly used in RNAi-based resistance reports 14,15,21,[23][24][25][26] . Protection against ToMV infection by dsRNA topical application.…”

Section: Inhibition Of Tomv-related Local Lesions Development In Hypementioning

confidence: 99%

See 1 more Smart Citation

siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants

Rêgo-Machado

Nakasu

Silva

et al. 2020

Sci Rep

View full text Add to dashboard Cite

A non-transgenic approach based on RNA interference was employed to induce protection against tomato mosaic virus (ToMV) infection in tomato plants. dsRNA molecules targeting the cp gene of ToMV were topically applied on plants prior to virus inoculation. Protection was dose-dependent and sequence-specific. While no protection was achieved when 0–16 µg dsRNA were used, maximum rates of resistance (60 and 63%) were observed in doses of 200 and 400 µg/plant, respectively. Similar rates were also obtained against potato virus Y when targeting its cp gene. The protection was quickly activated upon dsRNA application and lasted for up to 4 days. In contrast, no detectable antiviral response was triggered by the dsRNA from a begomovirus genome, suggesting the method is not effective against phloem-limited DNA viruses. Deep sequencing was performed to analyze the biogenesis of siRNA populations. Although long-dsRNA remained in the treated leaves for at least 10 days, its systemic movement was not observed. Conversely, dsRNA-derived siRNA populations (mainly 21- and 22-nt) were detected in non-treated leaves, which indicates endogenous processing and transport through the plant. Altogether, this study provides critical information for the development of novel tools against plant viruses; strengths and limitations inherent to the systems are discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Inhibition Of Tomv-related Local Lesions Development In Hypementioning

confidence: 99%

siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants

Rêgo-Machado

Nakasu

Silva

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Specifically, BCMV could be regulated when targeting CP and Nuclear Inclusion b (NIb) genes by application of dsRNA [11]. On the other hand, CP and Helper component-proteinase (HC-pro) genes were selected as target genes to control papaya ringspot virus so that dsRNAs conferred high resistance to plant against viral infection [19].…”

Section: Introductionmentioning

confidence: 99%

Double-stranded RNA confers resistance to pepper mottle virus in Nicotiana benthamiana

et al. 2021

View full text Add to dashboard Cite

RNA interference (RNAi) is a regulatory mechanism of gene expression mediated by small RNAs. By using the RNAi technique, exogenous double-stranded RNA (dsRNA) designed to target mRNA, suppresses target gene expression levels in plants. In this study, we adopted the RNAi mechanism as a tool to protect plants from viruses. We designed and synthesized several dsRNAs targeting the pepper mottle virus (PepMoV) genes HC-Pro and NIb. When used on Nicotiana benthamiana plants, these dsRNAs protected the plant against viral infection over a specific period. By optimizing dsRNA and virus injection time, the protection efficiency of dsRNA by targeting virus genes could be maximized. It seems that exogenous dsRNA-derived RNA-induced silencing complex was able to defend the host against viral infection instantly. Furthermore, each dsRNA designed to target different regions within a transcript had varying levels of effects on virus survival in the host plants. When targeting the middle part of both the NIb and HC-Pro genes using the dsRNAs, the highest viral growth inhibitory effect was observed. An RLM-5′ RACE was performed using plant leaves infected with PepMoV after dsRNA treatment and it was observed that most of the mRNA cleavages occurred close to the 3′ part within the dsRNA target position on the mRNA. These results suggest that the dsRNA tool can be used as a plant vaccine platform for crop protection.

show abstract

“…Several studies reported that foliar applications of dsRNAs induce plant resistance against target viruses ( Tenllado and Diaz‐Ruiz, 2001 ; Carbonell et al., 2008 ; Konakalla et al., 2016 ; Kaldis et al., 2018 ; Worrall et al., 2019 ; Vadlamudi et al., 2020 ). The effects of exogenously applied dsRNAs on conferring resistance against viruses have been reported in various host species, including tomato, tobacco, maize, papaya, cowpea, cucumber, watermelon, and squash against different viruses such as tobacco etch virus (TEV), tobacco mosaic virus (TMV), alfalfa mosaic virus (AMV), pepper mild mottle virus (PMMoV), potyvirus, bean common mosaic virus (BCMV), papaya ringspot virus (PRSV), and zucchini yellow mosaic virus (ZYMV) (reviewed in Dubrovina and Kiselev, 2019 ).…”

Section: The Possible Fate Of Dsrnas Into the Plant Cellmentioning

confidence: 99%

“…From these perspectives, exogenously applied dsRNAs to induce gene silencing have been perceived as another alternative to the genetic transformation that could provide similar benefits, without risking ecological stability and societal acceptance ( Dubrovina and Kiselev, 2019 ; Dalakouras et al., 2020 ). Indeed, several studies have reported that induction of RNAi mechanism by exogenous dsRNAs, short interfering RNAs (siRNAs), or hairpin RNAs (hpRNAs) has the potential to protect plants against plant pathogenic viruses ( Tenllado and Diaz‐Ruiz, 2001 ; Carbonell et al., 2008 ; Yin et al., 2009 ; Gan et al., 2010 ; Konakalla et al., 2016 ; Vadlamudi et al, 2020 ), fungi ( Koch et al., 2016 ; Wang et al., 2016 ; Wang et al., 2017 ), insects ( Baum et al., 2007 ; Li et al., 2013 ; Ghosh et al., 2017 ; Luo et al., 2017 ), mites, and nematodes (reviewed in Dubrovina and Kiselev, 2019 ; Dalakouras et al., 2020 ), which could eventually reduce the ecological footprints caused by chemical pesticides. However, it should be noted that most of the studies on the efficacy of exogenously applied dsRNAs were carried out under set experimental conditions, e.g., using detached leaves, targeting of transgenes, co-inoculation of dsRNAs with target viruses, etc, and have rarely been implemented under open-field conditions where several factors can largely affect their stability, uptake, and overall applicability.…”

Section: Introductionmentioning

confidence: 99%

Application of Exogenous dsRNAs-induced RNAi in Agriculture: Challenges and Triumphs

2020

View full text Add to dashboard Cite

In recent years, RNA interference (RNAi) machinery has widely been explored by plant biologists for its potential applications in disease management, plant development, and germplasm improvement. RNAi-based technologies have mainly been applied in the form of transgenic plant generation and host-induced-gene-silencing (HIGS). However, the approval of RNAi-based transgenic plants has always been challenging due to the proclaimed concerns surrounding their impacts on human health and the environment. Lately, exogenous applications of double-stranded RNAs (dsRNAs), short interfering RNAs (siRNAs), and hairpin RNAs (hpRNAs) has emerged as another technology that could be regarded as more eco-friendly, sustainable, and publicly acceptable than genetic transformation. Inside the plant cell, dsRNAs can undergo several steps of processing, which not only triggers RNAi machinery but may also involve transitive and systemic silencing, as well as epigenetic modifications. Therefore, along with the considerations of proper exogenous applications of dsRNAs, defining their final destination into plant cells is highly relevant. In this review, we highlighted the significance of several factors that affect dsRNA-induced gene silencing, the fate of exogenous dsRNAs in the plant cell, and the challenges surrounding production technologies, cost-effectiveness, and dsRNAs stability under open-field conditions. This review also provided insights into the potential applications of exogenous dsRNAs in plant protection and crop improvement.

show abstract

DsRNA-mediated protection against two isolates of Papaya ringspot virus through topical application of dsRNA in papaya

Cited by 35 publications

References 26 publications

siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants

siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants

Double-stranded RNA confers resistance to pepper mottle virus in Nicotiana benthamiana

Application of Exogenous dsRNAs-induced RNAi in Agriculture: Challenges and Triumphs

Contact Info

Product

Resources

About