DSC measurement of cartilage destruction caused by septic arthritis

Sillinger, T.; Than, Péter; Kocsis, Béla; Lőrinczy, D.

doi:10.1007/s10973-005-0870-8

Cited by 16 publications

(6 citation statements)

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“…The temperature was selected to stay below the denaturation temperature of organic matrix macromolecules (i.e., collagen and PG) around 50°C to 70°C. 30,31 The oven drying conditions are, therefore, expected to remove predominantly mobile water and have minimal effect on tightly bound water compartment, which requires much higher temperature to remove from the matrix. 30,31 The specimens were kept in a sealed plastic bag except when gravimetric and Raman analyses were carried out to avoid rehydration by the moisture in air.…”

Section: Serial Dehydration and Gravimetric Measurementsmentioning

confidence: 99%

Shortwave-infrared Raman spectroscopic classification of water fractions in articular cartilage ex vivo

Ünal

Akkuş

2018

J. Biomed. Opt.

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Water loss is an early onset indicator of osteoarthritis. Although Raman spectroscopy (RS) holds the potential for measurement of cartilage hydration, the knowledge of Raman OH-stretch bands of biological tissue is very limited. We assesed here the sensitivity of RS to identify and classify water types in the cartilage. Raman spectrum measurements over the high wavenumber range were employed to identify different water fractions in articular cartilage. Raman spectra were collected from wet and sequentially dehydrated cartilage along with pure collagen type II and chondroitin sulfate standards. OH-stretch band of cartilage is dominated by mobile water, up to 95% of total intensities. We identified six peaks in cartilage spectrum using second-derivative analysis: peaks at 3200 and 3650 cm-1 are associated with organic matrix (both collagen and proteglycan) and matrix-bound water molecules. Peaks at 3250, 3453, and 3630 cm-1 are associated with collagen and collagen-related water molecules, whereas the peak at 3520 cm-1 is associated with proteoglycan (PG) and PG-related water molecules. The current work is the first thorough analysis of the Raman OH-stretch band of the cartilage and with the knowledge generated by this study, it may now be possible to study on cartilage hydration by RS.

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Section: Serial Dehydration and Gravimetric Measurementsmentioning

confidence: 99%

Shortwave-infrared Raman spectroscopic classification of water fractions in articular cartilage ex vivo

Ünal

Akkuş

2018

J. Biomed. Opt.

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“…This was accompanied by a decrease in the total enthalpy from 0.55 J g -1 to 0.375 J g -1 on the first day. These findings highlight the usefulness of DSC to detect infection of these joints in the early stages [32].…”

Section: Disease Statesmentioning

confidence: 56%

“…Thermoanalytical methods can be used [32] to monitor the destruction of the cartilage in patients with bacterial arthritis over a period of time. DSC results for control samples indicated melting temperatures of 49.7 and 55 °C, which shifted to 57 and 63.15 °C on day 1 of the infection.…”

Section: Disease Statesmentioning

confidence: 99%

Thermoanalytical methods applied to medicine

Glass

Brown

2009

Pure and Applied Chemistry

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Thermoanalytical methods have found increasing application in medicine due to the improved sensitivity and usability of the available instrumentation. Studies have identified important findings applied to medicine, including information on the thermal properties of the skin and the effect of insertion into body cavities of implants and prosthetics. These studies have explored the thermal stability of various materials to provide insight into drug penetration in order to design drug delivery systems, which are not only safe but capable of delivering improved and predictable therapeutic outcomes for patients. Differential scanning calorimetry (DSC) has also been applied to the study of disease states such as diabetes, where changes in the collagen structure of the skin, which may lead to long-term complications in these patients, can be detected. Although these results may at this stage not have significant clinical implications, they do provide medical researchers with a starting point for future investigations. The application of these techniques has been further extended to examinations of body systems and other disease states. The key for the future will be the ability of these techniques not only to provide information on alterations in these biological systems, but also to determine whether these alterations are clinically relevant.

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“…Differential scanning calorimetry (DSC) has widely been established in the study of thermal decomposition of biological macromolecules and provides insight into the changes in the bonds between water and matrix macromolecules. Previous calorimetry studies of cartilage established calorimetric standards of the healthy cartilage [12–13] and demonstrated differences between various stages of osteoarthritis [14–15]. Ignat'eva et al .…”

Section: Introductionmentioning

confidence: 99%

“…observed three separable thermal denaturation events in one wider endotherm of knee-joint cartilage and demonstrated that the transition temperature of the infected cartilage produced by septic arthritis shifted toward higher temperatures. However, the authors did not report thermal transitions associated with the presence of two types of hydrogen bonds, which may relate to weakly and strongly bound water compartments [14]. …”

Section: Introductionmentioning

confidence: 99%

Effect of water content on enthalpic relaxations in porcine septal cartilage

Chae

Protsenko

Lavernia

et al. 2008

J Therm Anal Calorim

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Cartilage thermoforming is an emerging surgical technology which uses heat to accelerate stress relaxation in mechanically deformed tissue specimens. Heat induced shape change in cartilage is associated with complex thermo mechanical behavior of which the mechanisms are still a subject of debate. Differential scanning calorimetry (DSC) was used to characterize the threshold temperatures and enthalpies in cartilage as a function of water content. The DSC identified two enthalpic events in porcine nasal septal cartilage, which depend on the water content. The change in the water content of cartilage impacts the interactions between matrix macromolecules and water molecules, which may be associated with a bound-free water transformation (reversible process) and a denaturation of cartilage (irreversible process).

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DSC measurement of cartilage destruction caused by septic arthritis

Cited by 16 publications

References 0 publications

Shortwave-infrared Raman spectroscopic classification of water fractions in articular cartilage ex vivo

Shortwave-infrared Raman spectroscopic classification of water fractions in articular cartilage ex vivo

Thermoanalytical methods applied to medicine

Effect of water content on enthalpic relaxations in porcine septal cartilage

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