DSC evaluation of cross-link development induced by laccases in corneas

Morandim-Giannetti, Andreia de Araújo; Carvalho, Taleesa Souza; Neto, José Carlos de Andrade; Bersanetti, Patrícia Alessandra

doi:10.1007/s10973-022-11783-w

Cited by 1 publication

(1 citation statement)

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“…These enzymes catalyze the oxidation of several compounds, including phenolic compounds, amines, polycyclic aromatic hydrocarbons, methoxy-substituted phenols, and certain inorganic compounds. The reaction proceeds through phenoxyl radicals, reducing O 2 to H 2 O via electron transfer [9,12], hydrogen atom transfer, and ionic oxidation [13][14]. This process occurs at their active sites, which contain four copper atoms categorized into three types: T1 (mononuclear copper), T2 (typical copper), and T3 (coupled binuclear copper).…”

Section: Introductionmentioning

confidence: 99%

Novel Laccase Biosensors from Manihot Esculenta, Crantz -Associated Fungi: A Biotechnological Approach to Dopamine Quantification

Vieira,

Bersanetti,

Garcia

et al. 2024

Preprint

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This study investigates the association of endophytic fungi with the leaves and stems of cassava (Manihot esculenta Crantz), leading to the isolation and identification of 23 fungal strains. Of these, 18 fungi were associated with stems and 5 with leaves. Subsequent analysis revealed laccase activity in 7 fungi isolates, predominantly within those derived from stems. Notably, the fungus LV2, identified as a strain of Xylaria sp., exhibited the highest laccase activity (0.03 U.mg− 1.min− 1). Optimal LV2 fungus growth and enzyme activity conditions were established at 11 days, pH 6.70, and 29ºC (0.051 U.mg− 1.min− 1). Utilizing the LV2 enzymatic extract, we developed a chitosan-based enzyme biosensor with STMP crosslinking for immobilization to detect low dopamine concentrations. The biosensor demonstrated optimal performance at pH 5.1 and 47.8ºC, achieving a detection limit of 0.30 µmol.L− 1 for dopamine.

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