Drynaria total flavonoids decrease cathepsin K expression in ovariectomized rats

Abstract

ABSTRACT. This study investigated the effects of Drynaria total flavonoids on cathepsin K serum concentrations and gene expression, biomechanics and bone mineral density (BMD) of the tibial shaft in ovariectomized rat models of osteoporosis, and mechanism in the prevention and cure of osteoporosis. Seventy-two female SpragueDawley rats were divided into six groups. The rats in each group were subjected to gastric lavage after the model was established. The tibial shaft of the right hindlimb was obtained to measure the BMD. Serum cathepsin K concentrations were determined. The cathepsin K mRNA expression was also determined using fluorescent quantitative polymerase chain reaction. The three-point bending method was performed to measure the maximum bending load of the tibial shaft. The total flavonoid and normal groups had significant differences in serum cathepsin K concentrations compared with that in the estrogen group (P < 0.05). The total flavonoid and sham-operated groups also showed significant differences in cathepsin K mRNA expression compared with that in the normal group (P < 0.01). The maximum bending load of the rats in the total flavonoid group was significantly different from that in the estrogen group (P < 0.05) and the shamoperated and normal groups (P < 0.01). The high-dose total flavonoid group elicited a better effect on BMD than that by the medium-and low-dose groups (P < 0.05). Thus, Drynaria total flavonoids inhibited the serum cathepsin K concentration and increased the maximum bending load of the tibial shaft in ovariectomized rats.

Proteomic Analysis of Estrogen-Mediated Signal Transduction in Osteoclasts Formation

“…Palacios et al demonstrated that estrogen directly binds with ER α , a membrane receptor expressed on osteoclast precursors, and decreases the formation of TRAP-expressing osteoclasts [ 27 ]. Cathepsin K plays critical roles in osteoclast bone resorption; Shi et al reported that cathepsin K was increased in an animal model of postmenopausal osteoporosis [ 28 ]. In our study, we determined that RANK, TRAP, and cathepsin K were downregulated in an obvious manner in the osteoclast pathway via WikiPathways database matching.…”

Network Meta-analysis of Oral Chinese Patent Medicine in Treatment of Primary Osteoporosis

“…In the treatment of POP, the pathogenesis based on the theory of traditional Chinese medicine is mainly derived from the idea of "the kidneys promote bone growth" and "if the qi and blood are not clear, it is painful", and the treatment rules are mainly based on nurturing the kidney and improving the sperm, activating the blood and improving the gas and dissolving the stasis. Bone fragments in traditional medicine to supplement flavonoids [24][25][26] , icariin [27][28][29][30][31][32], and artificial tiger bone meal are active ingredients in the treatment of POP and may clinically improve osteoporosis symptoms with the approval of the CFDA (State Food and Drug Administration).…”

Research Progress in the Treatment of Osteoporosis with Chinese Medicine

“…Ang et al [18] found that naringin can inhibit the formation and bone resorption of OC (Osteoclast, OC), which is realized by inhibiting the expression of RANK, activating nuclear factor κB(NF-κB) and phosphorylating ERK. Shi Xiaolin et al [19] observed and studied the effects of total flavonoids from bone clavicle on CTSK serum concentration and left tibial metaphysis gene expression in ovariectomized rat model of osteoporosis. It was found that serum cathepsin K in ovariectomized rat model treated by total flavonoids from bone clavicle was inhibited, and the mRNA expression of cathepsin K in left tibial metaphysis was also decreased as its concentration decreased.…”