Dry-powder form of chitosan nanospheres containing influenza virus and adjuvants for nasal immunization

Dehghan, Solmaz; Kheiri, Masoumeh Tavassoti; Tabatabaiean, Mansoureh; Darzi, Simin Janitabar; Tafaghodi, Mohsen

doi:10.1007/s12272-013-0043-4

Cited by 32 publications

(36 citation statements)

References 60 publications

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“…Altogether, these results indicate the adequacy of polyelectrolyte complexation as a method to produce antigen-loaded polymeric nanoparticles, reinforcing results already available in the literature. In fact, several works reporting the preparation of nanoparticles using methodologies involving electrostatic interaction and using materials such as chitosan, its derivatives and alginate, have demonstrated to provide protection to various model antigens including inactivated influenza virus [59], bovine serum albumin [60], tetanus toxoid [61] and diphtheria toxoid [62].…”

Section: Cs/lbgsmentioning

confidence: 99%

Chitosan/sulfated locust bean gum nanoparticles: In vitro and in vivo evaluation towards an application in oral immunization

Braz

Grenha

Ferreira

et al. 2017

International Journal of Biological Macromolecules

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This work proposes the design of nanoparticles based on locus bean gum (LBG) and chitosan to be used as oral immunoadjuvant for vaccination purposes. LBG-based nanoparticles were prepared by mild polyelectrolyte complexation between chitosan (CS) and a synthesized LBG sulfate derivative (LBGS). Morphological characterization suggested that nanoparticles present a solid and compact structure with spherical-like shape. Sizes around 180-200nm and a positive surface charge between +9mV and +14mV were obtained. CS/LBGS nanoparticles did not affect cell viability of Caco-2 cells after 3h and 24h of exposure when tested at concentrations up to 1.0mg/mL. Two model antigens (a particulate acellular extract HE of Salmonella enterica serovar Enteritidis, and ovalbumin as soluble antigen) were associated to CS/LBGS nanoparticles with efficiencies around 26% for ovalbumin and 32% for HE, which resulted in loading capacities up to 12%. The process did not affect the antigenicity of the associated antigens. BALB/c mice were orally immunized with ovalbumin-loaded nanoparticles (100μg), and results indicate an adjuvant effect of the CS/LBGS nanoparticles, eliciting a balanced Th1/Th2 immune response. Thus, CS/LBGS nanoparticles are promising as antigen mucosal delivery strategy, with particular interest for oral administration.

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Section: Cs/lbgsmentioning

confidence: 99%

Chitosan/sulfated locust bean gum nanoparticles: In vitro and in vivo evaluation towards an application in oral immunization

Braz

Grenha

Ferreira

et al. 2017

International Journal of Biological Macromolecules

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“…However, mucoadhesion property of chitosan and TMC particles increased compared to alginate coated and uncoated particles 26 .Dehghan et al formulated a polymeric nanosphere nasal vaccine for influenza which enters the body through the inhalation route27 . In the study, they prepared dry nanoparticle powders of influenza vaccines with two other immunoadjuvants using chitosan as the carrier.…”

mentioning

confidence: 99%

“…The formulation demonstrated that the vaccine structure and characteristics of chitosan did not change after the formulation. The particles had a size of 581.1±32.6nm with mucoadhesive properties of chitosan that makes it suitable for nasal delivery of vaccine27 . Dry powder chitosan nanospheres may be an appropriate delivery system for nasal immunization of influenza, due to the nano size range, the ability for chitosan to adhere to mucosal membranes, and suitable release profile27 .…”

mentioning

confidence: 99%

Inhalable nanoparticulate powders for respiratory delivery

Muralidharan

Malapit

Mallory

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

184

123

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“…The obtained value of n for antigen was 0.454 and lied between 0.43 and 0.85; which indicated that the release of antigen followed Fickian diffusion model from the formulations (Dehghan et al. 2013 ).…”

Section: Resultsmentioning

confidence: 91%

“…CHT NPs were fabricated using an ionic gelation method (Xu & Du 2003 ; Dehghan et al. 2013 ). Briefly, 5% w/v solution of tripolyphosphate (TPP) in purified water, as a hardening agent, was emulsified in paraffin oil containing Span 80 and Tween 80 (3% v/v, 1:1 ratio) as emulsifier.…”

Section: Methodsmentioning

confidence: 99%

Immunoadjuvant potential of cross-linked dextran microspheres mixed with chitosan nanospheres encapsulated with tetanus toxoid

Pirouzmand

Khameneh

Tafaghodi

2016

Pharmaceutical Biology

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Context: Nasal mucosa is a desirable route for mucosal vaccine delivery. Mucosal co-administration of chitosan nanoparticles with absorption enhancers such as cross-linked dextran microspheres (CDM, Sephadex®) is a promising antigen delivery system.Objective: In the current study, the chitosan nanospheres loaded with tetanus toxoid (CHT:TT NPs) was prepared and characterized. The immune responses against tetanus toxoid after nasal administration of CHT:TT NPs alone or mixed with CDM were also determined.Materials and methods: Chitosan nanospheres were prepared by ionic gelation method. Particle size, releasing profile and antigen stability were evaluated by dynamic light scattering, diffusion chamber and SDS-PAGE methods, respectively. Rabbits were nasally immunized with different formulations loaded with 40 Lf TT. After three times immunizations with 2 weeks intervals, sera IgG titres and nasal lavage sIgA titres were determined.Results: Mean size of CHT NPs and CHT:TT NPs were 205 ± 42 nm and 432 ± 85 nm, respectively. The release profile showed that 42.4 ± 10.5% of TT was released after 30 min and reached to a steady state after 1.5 h. Stability of encapsulated TT in nanospheres was confirmed by SDS-PAGE. The antibody titres showed that CHT:TT NPs-induced antibody titres were higher than TT solution. CHT NPs mixed with CDM induced the systemic IgG and nasal lavage sIgA titres higher than intranasal administration of TT solution (p < 0.001).Discussion and conclusion: As the results indicated, these CHT:TT NPs when co-administered with CDM were able to induce more immune responses and have the potential to be used in mucosal immunization.

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Dry-powder form of chitosan nanospheres containing influenza virus and adjuvants for nasal immunization

Cited by 32 publications

References 60 publications

Chitosan/sulfated locust bean gum nanoparticles: In vitro and in vivo evaluation towards an application in oral immunization

Chitosan/sulfated locust bean gum nanoparticles: In vitro and in vivo evaluation towards an application in oral immunization

Inhalable nanoparticulate powders for respiratory delivery

Immunoadjuvant potential of cross-linked dextran microspheres mixed with chitosan nanospheres encapsulated with tetanus toxoid

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