Dry-milled flour rice ‘Seolgaeng’ harbors a mutated fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase2
Ju-Won Kang,
Sang-Kyu Lee,
Su-Hyeon Shim
et al.
Abstract:‘Seolgaeng’, an opaque-endosperm rice (Oryza sativa) mutant, is used to prepare high-quality dry-milled rice flour. The mutation causing its opaque-endosperm phenotype was unknown. Map-based cloning identified a missense mutation in the gene FRUCTOSE-6-PHOSPHATE 2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE 2 (OsF2KP2) in Seolgaeng. Transfer DNA insertion and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9)-induced f2kp2 mutants exhibited opaque endosperm. Rice harbo… Show more
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