Dry leaf extracts of Tinospora cordifolia (Willd.) Miers attenuate oxidative stress and inflammatory condition in human monocytic (THP-1) cells

Reddi, Kiran Kumar; Tetali, Sarada D.

doi:10.1016/j.phymed.2019.152831

Cited by 35 publications

(13 citation statements)

References 27 publications

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“…The antioxidative effects of HZTF may partially account for the underlying mechanism. NO and ROS, which are affected by the activation of the arachidonic acid pathway [ 58 , 59 ], were also significantly inhibited by HZTF as demonstrated in our in vitro experiments.…”

Section: Discussionsupporting

confidence: 67%

Chinese Medicine Huzhen Tongfeng Formula Effectively Attenuates Gouty Arthritis by Inhibiting Arachidonic Acid Metabolism and Inflammatory Mediators

Deng

Chen

et al. 2020

Mediators of Inflammation

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The Chinese herbal medicine, Huzhen Tongfeng Formula (HZTF), derived from traditional Chinese medicine (TCM) practice, has recognized therapeutic benefits for gouty arthritis (GA). HZTF is currently in the late stage of approval process as a new anti-GA drug application. However, the underlying mechanism of HZTF as an antigout medication is unclear. In this study, we combined network pharmacology and experimental validation approaches to elucidate the mechanism of action of HZTF. First, the relative drug-disease target networks were constructed and analyzed for pathway enrichment. Potential pathways were then validated by in vitro and in vivo experiments. We found that 34 compounds from HZTF matched 181 potential drug targets. Topology analysis revealed 77 core targets of HZTF, which were highly related to gout, following screening of KEGG pathway enrichment. Further analysis demonstrated that the arachidonic acid metabolic pathway was the most relevant pathway involved in the mechanism of HZTF. Validation experiments showed that HZTF significantly inhibited the inflammatory cell infiltration into gouty joints, improved the swelling of affected joints, and increased the pain threshold. HZTF significantly reduced the transcription and production of various cytokines and inflammatory mediators in vitro. In particular, cyclooxygenase (COX)-1, COX-2, and 5-lipoxygenase were simultaneously downregulated. In conclusion, our study suggests that the antigout mechanism of HZTF is associated with the inhibition of the arachidonic acid pathway, resulting in the suppression of inflammatory cytokines and mediators. These findings extend our understanding of the pharmacological action of HZTF, rationalizing the application HZTF as an effective herbal therapy for GA.

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Section: Discussionsupporting

confidence: 67%

Chinese Medicine Huzhen Tongfeng Formula Effectively Attenuates Gouty Arthritis by Inhibiting Arachidonic Acid Metabolism and Inflammatory Mediators

Deng

Chen

et al. 2020

Mediators of Inflammation

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“…Glutathione-s-transferase, a family of phase II detoxification enzyme that catalyzes the conjugation of glutathione (GSH) to different varieties of endogenous and exogenous electrophilic components (19). In the present study, there was more than 50% reduction in the concentration of glutathione S transferase in diabetes as compared to normal control.…”

Section: Discussionmentioning

confidence: 48%

Evaluation of anti-oxidative properties of <i>Tinosporacordifoliain Alloxan</i> induced diabetic Wistar rats

Thakur¹,

Padmadeo²,

Kumar³

2021

Biomedicine

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Introduction and Aim: Type 2 Diabetes is a complex heterogenous disorder with marked hyperglycemia, reduced insulin sensitivity, increased oxidative stress, and altered carbohydrate, fat and protein metabolism. Increased oxidative stress is a key factor for the reduced pancreatic ? cell arose due to prolonged hyperglycemia. The present investigation admits ethanolic extract of Tinosporacordifolia (250mg/kg/body weight) as a potent antihyperglycemic agent as well as antioxidant. Materials and methods: Wistar norvegicus rats of 180-200 gm were involved in the present study divided in to 5 groups. Each group has 5 rats. Diabetes was induced by Alloxan (100mg/kg.b.wt). Administration of T.cordifolia (250mg/kg/b.wt) done in the desired group for 10,20, and 30 days respectively. Fasting blood collected for plasma glucose and the organs were collected for the antioxidant assay. Results: Each group showed significant recovery in the glucose and antioxidant parameters like Glutathione, Catalase, Ascorbate etc., Statistical analysis was done by Tukey multiple range test compared with entire column after ANOVA. Level of significance was denoted for diabetic Vs treated group having p values *** p<0.05, **p<0.01, *p<0.001, NS-Non-significant, >0.05. Conclusion: Relying on the Herbal extract of T.cordifolia would be one of the best options for controlling glucose concentration, enhanced immunity, and getting rid of unnecessary free radicals which increases aging. The plant under investigation is a reservoir of countless secondary metabolites.

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“…In THP-1 cells, both forms of extracts decreased AAinduced ROS generation by increasing catalase enzyme activity. The results show that TC leaf extracts possess antioxidant and anti-inflammatory properties by upregulation of antioxidant enzymes in activated human monocytic (THP-1) cells and attenuation of NF-κB nuclear translocation (30).…”

Section: Anti-inflammatory Propertiesmentioning

confidence: 94%

Promising implications of Tinospora cordifolia utilization and its functional significance: A review

Khan¹,

Katiyar²,

Nema³

2021

IJAM

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Tinospora cordifolia (TC) a traditional plant used in Indian Ayurvedic medicine widely. Different parts of this plant such as stem, roots, leaves are used for treatment and control of various health issues as well as an ingredient in functional foods. It possesses many health promoting and protecting properties. The present article is a critical review providing a comprehensive discussion on therapeutic application and functional significance of TC. The aim of this review is to develop a clear understanding of these issues. Specifically coverage in this review are published information in the areas of Diabetic retinopathy, Free radical scavenging, Anti-Diabetic, Cognition enhancing, Anti-inflammatory, Anti-allergic, Immunomodulatory and Anti-cancer, Management of Hay Fever, Antioxidant, Hypoglycaemic and Hypolipidaemic, Osteoblasts formation, Immunomodulatory, Immunostimulatory, Protection from Skeletal Muscle Atrophy, Gastroprotective, Antiosteoporotic etc. Uses of TC as plant digestive enzyme and as supplementation in formulation of various functional foods are also compiled for the information and knowledge of food scientists and technologists. The future scope of the review remains in exploiting TC so as to enable new and effective formulation in disease eradication. Further studies are necessary, however, to investigate the best practices for the promising implications of TC utilization and its functional significance.

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Dry leaf extracts of Tinospora cordifolia (Willd.) Miers attenuate oxidative stress and inflammatory condition in human monocytic (THP-1) cells

Cited by 35 publications

References 27 publications

Chinese Medicine Huzhen Tongfeng Formula Effectively Attenuates Gouty Arthritis by Inhibiting Arachidonic Acid Metabolism and Inflammatory Mediators

Chinese Medicine Huzhen Tongfeng Formula Effectively Attenuates Gouty Arthritis by Inhibiting Arachidonic Acid Metabolism and Inflammatory Mediators

Evaluation of anti-oxidative properties of <i>Tinosporacordifoliain Alloxan</i> induced diabetic Wistar rats

Promising implications of Tinospora cordifolia utilization and its functional significance: A review

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