Dry Blood Spot sample collection for post-exposure monitoring of chemical warfare agents – In vivo determination of phosphonic acids using LC-MS/MS

Aviram, Lilach Yishai; Magen, Miriam; Chapman, Shira; Cohen, Adi Neufeld; Lazar, Shlomi; Dagan, S.

doi:10.1016/j.jchromb.2018.06.035

Cited by 13 publications

(4 citation statements)

References 28 publications

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“…With the DBS technique, sampling is easy and fast without the need for trained professionals. However, the small amounts of blood drawn (∼20–40 µL) make this approach more analytically challenging in terms of sample preparation and sensitivity compared with traditional blood sampling [26, 27]. Blood drops were collected in sterile mode.…”

Section: Methodsmentioning

confidence: 99%

Detection of recombinant Spike protein in the blood of individuals vaccinated against SARS‐CoV‐2: Possible molecular mechanisms

Brogna,

Cristoni,

Marino

et al. 2023

Proteomics Clinical Apps

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PurposeThe SARS‐CoV‐2 pandemic prompted the development and use of next‐generation vaccines. Among these, mRNA‐based vaccines consist of injectable solutions of mRNA encoding for a recombinant Spike, which is distinguishable from the wild‐type protein due to specific amino acid variations introduced to maintain the protein in a prefused state. This work presents a proteomic approach to reveal the presence of recombinant Spike protein in vaccinated subjects regardless of antibody titer.Experimental designMass spectrometry examination of biological samples was used to detect the presence of specific fragments of recombinant Spike protein in subjects who received mRNA‐based vaccines.ResultsThe specific PP‐Spike fragment was found in 50% of the biological samples analyzed, and its presence was independent of the SARS‐CoV‐2 IgG antibody titer. The minimum and maximum time at which PP‐Spike was detected after vaccination was 69 and 187 days, respectively.Conclusions and clinical relevanceThe presented method allows to evaluate the half‐life of the Spike protein molecule “PP” and to consider the risks or benefits in continuing to administer additional booster doses of the SARS‐CoV‐2 mRNA vaccine. This approach is of valuable support to complement antibody level monitoring and represents the first proteomic detection of recombinant Spike in vaccinated subjects.

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Section: Methodsmentioning

confidence: 99%

Detection of recombinant Spike protein in the blood of individuals vaccinated against SARS‐CoV‐2: Possible molecular mechanisms

Brogna,

Cristoni,

Marino

et al. 2023

Proteomics Clinical Apps

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show abstract

“…Dried material is considered non-infectious. Therefore, procedures and devices for the shipment of dried blood and plasma spotted onto filter paper were introduced that allow protein-adduct analysis [64] , [79] , [80] , [81] . The dried material showed excellent stability and the solid support did not cause any interference, thus highlighting an important alternative for simplified sample transport, storage and analysis.…”

Section: Instrumental and Methodological Progressmentioning

confidence: 99%

Poisoning by organophosphorus nerve agents and pesticides: An overview of the principle strategies and current progress of mass spectrometry-based procedures for verification

John¹,

Thiermann²

2021

Journal of Mass Spectrometry and Advances in the Clinical Lab

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Highlights Evidence of poisoning with organophosphorus (OP) nerve agents requires biomedical verification. OP nerve agents undergo common biotransformation pathways producing valuable biomarkers. Internationally accepted methods target remaining poison, hydrolysis products and protein-adducts. Mass spectrometry-based methods provide optimum selectivity and sensitivity for identification. Methods, strategies, current proceedings, quality criteria and real cases of poisoning are presented.

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“…After inhalational or cutaneous exposure in humans or animals, absorbed OPNA inhibits the acetylcholinesterase (AChE) enzyme, which controls the hydrolysis of the neurotransmitter acetylcholine. This inhibition causes the accumulation of acetylcholine at the neuromuscular junction that leads to a cholinergic crisis characterized by muscarinic symptoms, including salivation, lacrimation, urination, defecation, gastrointestinal distress, and emesis, known by the mnemonic “SLUDGE” [ 2 , 4 , 6 , 7 ]. VX (C 11 H 26 NO 2 PS) is one of the lethal OPNAs, and its median lethal dose (LD 50 ) of percutaneous exposure is less than 10 mg for a 70 kg person [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a molecularly imprinted polymer-based electrochemical sensor for the selective detection of nerve agent VX metabolite ethyl methylphosphonic acid in human plasma and urine samples

Sezigen,

Kaya,

Bakirhan

et al. 2024

Anal Bioanal Chem

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This study focuses on the detection of ethyl methyl phosphonic acid (EMPA), a metabolite of the banned organophosphorus nerve agent VX. We developed an electrochemical sensor utilizing the molecularly imprinted polymer (MIP) based on 4-aminobenzoic acid (4-ABA) and tetraethyl orthosilicate for the selective detection of EMPA in human plasma and urine samples. The 4-ABA@EMPA/MIP/GCE sensor was constructed by a thermal polymerization process on a glassy carbon electrode and sensor characterization was performed by cyclic voltammetry and electrochemical impedance spectroscopy. The 4-ABA@EMPA/MIP/GCE sensor demonstrated impressive linear ranges 1.0 × 10–10 M–2.5 × 10–9 M for the standard solution, 1.0 × 10–10 M–2.5 × 10–9 M for the urine sample, and 1.0 × 10–10 M–1 × 10–9 M of EMPA for the plasma sample with outstanding detection limits of 2.75 × 10−11 M (standard solution), 2.11 × 10−11 M (urine), and 2.36 × 10−11 M (plasma). The sensor exhibited excellent recovery percentages ranging from 99.86 to 101.30% in urine samples and 100.62 to 101.08% in plasma samples. These findings underscore the effectiveness of the 4-ABA@EMPA/MIP/GCE as a straightforward, highly sensitive, and selective interface capable of detecting the target analyte EMPA in human plasma and urine samples.

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Dry Blood Spot sample collection for post-exposure monitoring of chemical warfare agents – In vivo determination of phosphonic acids using LC-MS/MS

Cited by 13 publications

References 28 publications

Detection of recombinant Spike protein in the blood of individuals vaccinated against SARS‐CoV‐2: Possible molecular mechanisms

Detection of recombinant Spike protein in the blood of individuals vaccinated against SARS‐CoV‐2: Possible molecular mechanisms

Poisoning by organophosphorus nerve agents and pesticides: An overview of the principle strategies and current progress of mass spectrometry-based procedures for verification

Development of a molecularly imprinted polymer-based electrochemical sensor for the selective detection of nerve agent VX metabolite ethyl methylphosphonic acid in human plasma and urine samples

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