Dry biobanking as a conservation tool in the Anthropocene

Saragusty, Joseph; Anzalone, Debora Agata; Palazzese, Luca; Arav, Amir; Patrizio, Pasquale; Gosálvez, Jaime; Loi, Pasqualino

doi:10.1016/j.theriogenology.2020.01.022

Cited by 16 publications

(17 citation statements)

References 71 publications

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“…The nuclei of freeze-dried (FD) mouse spermatozoa have a strong tolerance against environmental changes 9 ; healthy offspring were obtained from FD sperm stored for more than one year in a desk drawer without controlling room temperature 10 and in the International Space Station for more than 5 years 11 , 12 . Thus, freeze drying could be the best way to preserve genetic resources for a long period in a safe, low-cost, and location-independent manner 13 , 14 . However, to date, the only cells that have produced offspring after freeze drying are mature spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

Healthy cloned offspring derived from freeze-dried somatic cells

et al. 2022

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Maintaining biodiversity is an essential task, but storing germ cells as genetic resources using liquid nitrogen is difficult, expensive, and easily disrupted during disasters. Our aim is to generate cloned mice from freeze-dried somatic cell nuclei, preserved at −30 °C for up to 9 months after freeze drying treatment. All somatic cells died after freeze drying, and nucleic DNA damage significantly increased. However, after nuclear transfer, we produced cloned blastocysts from freeze-dried somatic cells, and established nuclear transfer embryonic stem cell lines. Using these cells as nuclear donors for re-cloning, we obtained healthy cloned female and male mice with a success rate of 0.2–5.4%. Here, we show that freeze-dried somatic cells can produce healthy, fertile clones, suggesting that this technique may be important for the establishment of alternative, cheaper, and safer liquid nitrogen-free bio-banking solutions.

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Section: Introductionmentioning

confidence: 99%

Healthy cloned offspring derived from freeze-dried somatic cells

et al. 2022

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“…While this estimate might have changed since then, it is evident that more widespread sampling would substantially push the need for additional resources. To minimize costs, dry storage at more ambient temperatures has been frequently proposed as a possible alternative (Anchordoquy & Molina, 2007; Lou et al, 2014; Saragusty et al, 2020). The dehydration of DNA reduces molecular mobility and minimizes nuclease activity and hydrolytic damage.…”

Section: Building High‐quality Biodiversity Tissue Archivesmentioning

confidence: 99%

“…A similar test with human sperm showed that the degree of DNA degradation was negligible 1 week after lyophilization (Gianaroli et al, 2012). Based on such findings, Saragusty et al (2020) have argued that dry storage might be the only realistic approach for exhaustive biobanking and ensure the preservation of gametes for conservation purposes. However, many outstanding questions remain and systematic (long‐term) studies of DNA stability in dry conditions are still needed (Anchordoquy & Molina, 2007).…”

Section: Building High‐quality Biodiversity Tissue Archivesmentioning

confidence: 99%

Opportunities and challenges for high‐quality biodiversity tissue archives in the age of long‐read sequencing

Blom

2021

Molecular Ecology

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With the continued development and decrease in costs, highthroughput sequencing has radically changed the field of molecular ecology during the past decade. Until recently, genomic resources such as chromosome scale assemblies were only available for a few model organisms and the lack of reference genomes hampered the widespread adoption of whole-genome sequencing approaches. The so-called third generation of high-throughput sequencing (van Dijk et al., 2018) is now challenging this paradigm and is shifting focus from short-to long-read sequencing (LRS). Recently introduced LRS platforms, such as Pacific Biosciences (PacBio) Sequel II and Oxford Nanopore Technologies

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“…Healthy offspring were obtained from FD sperm stored in a desk drawer of laboratory 6 or even on the International Space Station 7,8 , as well as from transported FD sperm on the postcard 9 . Thus, freeze drying could be the best way to preserve genetic resources for a long period in a safe, low-cost, and location-independent manner 10,11 . However, it is di cult to collect spermatozoa from infertile animals, such as aged animals, animals with their reproductive organs accidentally damaged, or immature males.…”

Section: Introductionmentioning

confidence: 99%

Production of mouse offspring from zygotes fertilized with freeze-dried spermatids

Wakayama

Ito

Ooga

et al. 2022

Preprint

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Recently, mice have been produced from Freeze-drying (FD) somatic cells by nuclear transfer, but the success rate was very low compared to FD spermatozoa. Since spermatozoa, unlike somatic cells, are haploid cell and their nuclei were hardened by protamine, it is unclear which is responsible for tolerance to FD treatment. Here, we attempt to produce offspring from FD spermatid, a haploid sperm progenitor cell, but nuclei were not yet replaced by protamine as same as somatic cells. We developed the method to collect FD spermatids from testicular suspension. When FD spermatids were injected into oocytes, healthy offspring were obtained, despite the significantly reduced success rate compared to FD spermatozoa. Offspring were also obtained from FD spermatids derived from immature male mice, which have not yet produced spermatozoa. These results indicate that nuclear protaminization, rather than haploid nuclei, is the key process responsible for tolerance to FD treatment.

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Dry biobanking as a conservation tool in the Anthropocene

Cited by 16 publications

References 71 publications

Healthy cloned offspring derived from freeze-dried somatic cells

Healthy cloned offspring derived from freeze-dried somatic cells

Opportunities and challenges for high‐quality biodiversity tissue archives in the age of long‐read sequencing

Production of mouse offspring from zygotes fertilized with freeze-dried spermatids

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