DRw53: 2-D Gel Patterns

Knowles, Robert W.; Kienzle, Bernadette; Winchester, Robert; Matthews, Erin; Bouhallier, O.; Fauchet, R.; Marshall, William H.; Monos, Dimitri; Amesland, Françoise; Mazzilli, Maria Christina; Henry, Marie-Anne; Perrier, P.; Dejelo, Cora; Fernández, Nelson; Nepom, Barbara S.; Knitter-Jack, Nan; Nepom, Gerald T.; Kamoun, Malek; Zmijewski, Chester M.; Lanchbury, Jerry S.

doi:10.1007/978-1-4612-3552-1_90

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…Likewise, the only reagent submitted to the workshop thought to be DRw53, DRBCspecific, DUP702 (PL3), has been shown to react with both DRBI and DRB4 gene products in DR7 and DR9 cells, but only with DRw53-DRB4 in DR4 cells (6).This is an example of a mAb recognising an epitope expressed on both DRBl and DRB4 gene products in different cell types.…”

Section: Class Itmentioning

confidence: 99%

A preliminary analysis of the 11.th International HistocompatibilitY Workshop monoclonal antibodies *

1990

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In the 11th International Histocompatibility Workshop prescreening study, HLA Class I and Class II monoclonal antibodies (mAb) were tested on panels of peripheral T and B cells and homozygous typing cell (HTC) lymphoblastoid lines. The 102 Class I mAb, of which 64 were new, were screened on 63 HTC lines and 20 peripheral T cells. The 144 Class II mAb were tested on 63 HTC lines, 20 peripheral B and 10 peripheral T cells. For Class I, 45 of the 102 mAbs tested proved to have clear and identifiable reaction patterns on both PBL and HTC lines and included several interesting new HLA-A and -C locus antibodies. Of the 144 Class II mAb, of which 99 were new, 18 DR, 22 DQ and 7 DP antibodies were judged excellent. The DR antibodies included some which were antigen-specific but the majority were complex antibodies, while the DQ antibodies, as previously, identified the majority of DQ antigens both singly and in combinations. The encouraging number of DP antibodies was interesting in the limited range of sites they appear to detect.

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Section: Class Itmentioning

confidence: 99%

A preliminary analysis of the 11.th International HistocompatibilitY Workshop monoclonal antibodies *

1990

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“…Since our initial description of the DR fl chain polymorphism, two-dimensional PAGE has been widely used to identify molecular variants belonging to DR2, DR4, DR5, DRw6, DR7, and DRw52. So far, this is one of the best typing procedures for the identification of DQ a and DP a variants, with eight and two alleles, respectively McDevitt, 1979, 1980;Knowles, 1989;Charron and Fernandez, 1990). The theoretical limitation is that in some instances conservative amino acid substitution will not be detected electrophoretically and thus will not be resolved by two-dimensional PAGE.…”

Section: B Biochemistrymentioning

confidence: 99%