Drugs that block smooth muscle contraction inhibit in vivo ovulation in hamsters

Martin, Gary G.; Talbot, Prudence

doi:10.1002/jez.1402160317

Cited by 39 publications

(12 citation statements)

References 28 publications

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“…It has been demonstrated that these cells contract prior to ovulation [5,25,26]. This contraction is correlated with the development of a V-shaped constriction in the basis of the Graafian follicle, which is required for ovulation [27]. Self et al [28] showed in the hamster that the theca externa is a functional contractile system that responds to a Ca2+ influx.…”

Section: Discussionmentioning

confidence: 97%

In Vitro Study of the Contractility of the Wall of the Preovulatory Follicle in the Japanese Quail1

Nassauw

Sys

Harrisson

et al. 1993

Biology of Reproduction

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In the present study, we examined in vitro the potassium-induced contractions of isolated parts of the wall of the quail preovulatory follicle through use of an electromagnetic force-length transducer system. We studied longitudinal and transverse strips of the whole wall, of the theca, and of the outer layer (tunica albuginea and surface epithelium). It was observed that force development and shortening of strips of the whole wall and those of the theca are similar. Statistical analysis of maximal isometric force, peak shortening length, and percentage of reduction of follicular volume showed that the orientation of strips is without importance. Results from different orientations were pooled, and the different layers were compared statistically. The general result of this analysis was that the contractile activity of the outer layer was significantly lower than the performance of either the whole wall or the theca. We concluded that contraction of the follicular wall is mainly dependent on the contractile activity of the theca externa. This contractile system is comparable to that of mammals. We suggest that the main function of the theca externa during ovulation is to maintain a constant intrafollicular pressure.

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Section: Discussionmentioning

confidence: 97%

In Vitro Study of the Contractility of the Wall of the Preovulatory Follicle in the Japanese Quail1

Nassauw

Sys

Harrisson

et al. 1993

Biology of Reproduction

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“…Espey, 1980). Other prostaglandininduced phenomena that have been implicated in follicular rupture include the mobilization of fibroblasts (Espey et al, 1981) and follicular contractions (Martin and Talbot, 1981).…”

Section: Discussionmentioning

confidence: 99%

An ultrastructural study of preovulatory apical development in mouse ovanan follicles: Effects of indomethacin

Downs

Longo

1983

Anat. Rec.

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The effects of the prostaglandin synthesis inhibitor, indomethacin, on the preovulatory morphology of apical follicle walls have been examined by transmission electron microscopy. Immature mice, superovulated with 5 IU pregnant mare serum (PMS) followed 40 hours later by 80 IU luteinizing hormone (LH) were treated with either 10 mg/kg indomethacin or an equivalent volume of the indomethacin vehicle 10 minutes prior to LH. Follicular apices from both groups were compared at 12 hours post-LH. Indomethacin treatment suppressed many of the morphological changes normally occurring in the apex during preovulatory development. Whereas apices from vehicle-treated animals demonstrated marked deterioration, dissociation, and thinning of tissue, the cell layers of apices from indomethacin-treated animals remained thickened and tightly packed, with limited signs of disruption. The results presented herein are consistent with the idea that prostaglandins are essential mediators of ovulation and suggest that these lipids augment apical rupture by mobilizing granulosa cells and stimulating the loss of connective tissue elements.

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“…That this event plays a role in ovulation has been suggested by Martin & Talbot (1981b), who demonstrated that ovulation in hamsters could be inhibited by a variety of drugs known to inhibit smooth muscle contraction.…”

Section: Introductionmentioning

confidence: 90%

Relaxation-induced contraction of smooth muscle surrounding hamster ovarian follicles

Self¹,

Koch²,

Schroeder³

1989

Reproduction

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Summary. Changes in intrafollicular pressure and follicular diameter resulting from injecting or withdrawing fluid from the antrum were measured in preovulatory follicles and used as an assay for changes in tension in the follicular wall by applying the Laplace relationship for thin-walled spheres. Passive length\p=n-\tension curves were constructed from pressure\p=n-\volume measurements to establish baseline wall stiffness. Any subsequent change in pressure could then be compared to the length\p=n-\tension curves to evaluate whether it arose from active tension development or from passive stretch. When intact follicles (1\p=n-\2 h before ovulation) were subjected to release of passive stretch, they exhibited a contractile response that lasted 15 sec\p=n-\2min and was characterized by cyclic increases and decreases in tension, with a period of 1 cycle every 2\ p=n-\ 3 sec. The probability of activating a response in the tissue was most strongly correlated with the rate of release of passive stretch. Intrafollicular pressures generated during active contractile responses sometimes reached 80 mmHg (10\m=.\64mPa), corresponding to a wall tension of 5332 dynes/cm (5\m=.\332N/m) (for a 1 mm follicle) and were clearly well above the passive length\p=n-\tension curves. Passive stretching of the follicular wall during a contractile response to 5-hydroxytryptamine stimulation resulted in large reductions in active wall tension for the duration of the stretch. These results are consistent with a stretch-activated inhibition of contractile events.

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Drugs that block smooth muscle contraction inhibit in vivo ovulation in hamsters

Cited by 39 publications

References 28 publications

In Vitro Study of the Contractility of the Wall of the Preovulatory Follicle in the Japanese Quail1

In Vitro Study of the Contractility of the Wall of the Preovulatory Follicle in the Japanese Quail1

An ultrastructural study of preovulatory apical development in mouse ovanan follicles: Effects of indomethacin

Relaxation-induced contraction of smooth muscle surrounding hamster ovarian follicles

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