Drug susceptibility testing of Mycobacterium tuberculosis with nitrate reductase assay

Çoban, Ahmet Yılmaz; Bırınci, Asuman; Ekinci, Bora; Durupinar, Belma

doi:10.1016/j.ijantimicag.2004.02.027

Cited by 37 publications

(19 citation statements)

References 12 publications

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“…With input from MSLI, the INS chose the Griess method. This method is a rapid colorimetric method that uses Lowenstein-Jensen (LJ) medium prepared with antimicrobial drugs ( Figure 3) (19). Previously the method was validated as an indirect method; however, INS opted to implement it as a direct method, i.e., it is performed directly with sputum.…”

Section: Strategy To Scale-up Laboratory Servicesmentioning

confidence: 99%

Scale-up of Multidrug-Resistant Tuberculosis Laboratory Services, Peru

Shin

Yagui

Ascencios

et al. 2008

Emerg. Infect. Dis.

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Over the past 10 years, the Peruvian National Tuberculosis (TB) Program, the National Reference Laboratory (NRL), Socios en Salud, and US partners have worked to strengthen the national TB laboratory network to support treatment of multidrug-resistant TB. We review key lessons of this experience. The preparation phase involved establishing criteria for drug susceptibility testing (DST), selecting appropriate DST methods, projecting the quantity of DST and culture to ensure adequate supplies, creating biosafe laboratory facilities for DST, training laboratory personnel on methods, and validating DST methods at the NRL. Implementation involved training providers on DST indications, validating conventional and rapid fi rst-line DST methods at district laboratories, and eliminating additional delays in specimen transport and result reporting. Monitoring included ongoing quality control and quality assurance procedures. Hurdles included logistics, coordinating with policy, competing interests, changing personnel, communications, and evaluation. Operational research guided laboratory scaleup and identifi ed barriers to effective capacity building.

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Section: Strategy To Scale-up Laboratory Servicesmentioning

confidence: 99%

Scale-up of Multidrug-Resistant Tuberculosis Laboratory Services, Peru

Shin

Yagui

Ascencios

et al. 2008

Emerg. Infect. Dis.

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“…Esta prueba ha mostrado buena sensibilidad y especificidad (>90 %) (14) al compararla con la prueba de proporciones múltiples considerada como la de referencia (13,14), y no requiere equipos ni reactivos costosos, por lo que ha sido propuesta como una herramienta fenotípica alternativa, útil para la detección rápida y precisa de la resistencia de M. tuberculosis a fármacos de primera línea (13,14,(23)(24)(25)(26)(27). Además, la utilización de medios sólidos (Löwenstein-Jensen y agar Middlebrook 7H11), a los que se les agregan el nitrato de potasio (KNO 3 ) como sustrato de la enzima y los medicamentos a evaluar, reduce el riesgo de producción de aerosoles, presente cuando se emplea un medio líquido (28).…”

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Utilidad de la prueba de la nitrato reductasa para la detección rápida de resistencia en Mycobacterium tuberculosis

González¹,

Pedraza²,

Murcia³

2013

biomedica

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“…Detecting tuberculosis and identifying MDR Mycobacterium tuberculosis strains by conventional methods is difficult because of the low growth rate of the causative agent. Therefore, rapid and efficient methods are needed for the control of this disease (3)(4)(5)(6)13).…”

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Susceptibilities of Mycobacterium tuberculosis to Isoniazid and Rifampin on Blood Agar

Çoban¹,

Bılgın²,

Uzun

et al. 2005

J Clin Microbiol

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In this study, blood agar was used instead of 7H10 agar for the susceptibility testing of 34 clinical isolates of Mycobacterium tuberculosis to isoniazid (INH) and rifampin (RIF) in accordance with the NCCLS. The BACTEC 460 TB system (Becton Dickinson, Sparks, Md.) was used as a "gold standard." Results for both media were in agreement for RIF and INH at 100 and 94.1%, respectively. For INH, the specificity, sensitivity, positive predictive value, and negative predictive value were found to be 71.4, 100, 93.1, and 100%, respectively, while these values were 100% for RIF. In addition, the results of the susceptibility test performed with blood agar were obtained on day 14 of incubation. In conclusion, results were obtained much earlier with blood agar (2 weeks) than with 7H10 agar (3 weeks), and the results of this study suggest that blood agar may be used as an alternative medium for the susceptibility testing of M. tuberculosis to INH and RIF.The increasing incidence of multidrug-resistant tuberculosis (MDR-TB) produces serious problems in developed and especially in developing countries. Detecting tuberculosis and identifying MDR Mycobacterium tuberculosis strains by conventional methods is difficult because of the low growth rate of the causative agent. Therefore, rapid and efficient methods are needed for the control of this disease (3-6, 13).Several manufacturers have directed considerable effort toward the development of rapid and efficient systems for the growth, detection, and susceptibility testing of mycobacteria. Two of these systems, the BACTEC 460 TB (Becton Dickinson, Sparks, Md.) and the BACTEC MGIT 960 (Becton Dickinson), have become available for the susceptibility testing of M. tuberculosis and are also recommended by the NCCLS. Although the time to detect M. tuberculosis from clinical specimens can be shortened and the results for susceptibility testing can be obtained in 4 to 7 days by the use of these systems, they are labor-intensive and expensive and generate radioactive waste (2-4, 12). Therefore, several methods based on liquid media have been developed by different investigators for the susceptibility testing of M. tuberculosis (2, 4-6, 10, 11, 13, 14).Drancourt et al. (7) investigated the effectiveness of blood agar for primary isolation of M. tuberculosis. They reported that M. tuberculosis can easily grow on blood agar in 1 to 2 weeks and that this medium has been routinely used instead of egg-based medium in the inoculation of 10,000 samples in a year for the diagnosis of tuberculosis, with the same results being obtained.In this study, we evaluated the performance of blood agar for susceptibility testing of 34 M. tuberculosis clinical isolates to isoniazid (INH) and rifampin (RIF) by using the proportion method.Bacterial isolates. Thirty-four clinical isolates of M. tuberculosis were examined in this study, and H37Rv and H37Ra were also included as control strains. Drug susceptibility patterns of all isolates were previously detected by the BACTEC 460 TB system, and a standard prot...

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Drug susceptibility testing of Mycobacterium tuberculosis with nitrate reductase assay

Abstract: In this study, we have evaluated the broth microdilution method (BMM) 100, 100, 96.9, and 90.2%, while specificity was 100, 85.7, 90.9, and 100% for INH, RIF, STR, and ETM, respectively

Cited by 37 publications

References 12 publications

Scale-up of Multidrug-Resistant Tuberculosis Laboratory Services, Peru

Scale-up of Multidrug-Resistant Tuberculosis Laboratory Services, Peru

Utilidad de la prueba de la nitrato reductasa para la detección rápida de resistencia en Mycobacterium tuberculosis

Susceptibilities of Mycobacterium tuberculosis to Isoniazid and Rifampin on Blood Agar

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