Drug Resistance Pattern of Blood Culture Isolates in a Tertiary Care Hospital

Abstract

Aim of the study: This study was carried out to find out In vitro susceptibility & resistance pattern of isolates were carried out by Kirby-Bauer disc diffusion method. Material and Methods: - The present study was conducted in Department of Microbiology in Era’s Lucknow Medical College and hospital. During the period of six months, 638 blood samples from septicemia suspected patients were received and processed routinely. Out of 638 blood culture, 90 (14.10%) were found positive for culture growth in which 38% were Gram’s positive and 62% were Gram’s negative. Out of 90 positive culture overall proportion of female (47) were found to be significantly higher them male (43). Results: Among 90 total pathogenic cases, 47(52.22%) were females and 43 (47.78%) were males. Oyekale T Oluwalana et al, (2022) [26] also showed similar data i.e, 57% of males and 42.4% female. In our study, 37 samples belong to age group from newborn to 25 years age (41.11%), 24 belongs to group 26 to 50 years age (26.67%), while 29 from age group 51 to 90 years (32.22%) were suspected of septicaemia. Staphylococcus aureus, coagulasenegative staphylococcus (CoNS) and Enterococcus species showed 100 % sensitivity to Vancomycin, Teicoplanin & Linezolid and Enterococcus species showed 90% sensitivity to Gentamicin, 87% sensitivity to Linezolid, moderately sensitivity 67% to Gentamycin and 80% to Teicoplanin and low sensitivity showed by Ciprofloxacin, Amoxiclav, 100% Resistance showed to Cefoxitin in staphylococcus aureus and coagulase-negative staphylococcus, enterococcus species. showed high sensitivity to Vancomycin (98%), Teicoplanin (98%), Chloramphenicol (89%) and (100%) sensitivity to linezolid. High level gentamycin resistance was 44%. Conclusion: Our study observed 100 % sensitivity to Tigecycline, Colistin and Piperacillin-Tazobactam in Escherichia coli, netilmicin was moderately sensitive to klebsiella and Escherichia coli. Higher resistance observed by Cefepime, Cefoperazone, Imipenem, Ceftriaxone.