Drug resistance in Plasmodium berghei. I. Chloroquine resistance

Peters, W.

doi:10.1016/0014-4894(65)90012-3

Cited by 316 publications

(144 citation statements)

References 4 publications

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“…The repository activity of the extract, fractions, and pyrimethamine (daraprim) was assessed by using the method described by Peters (1965). The mice were randomly divided into five groups of six mice each.…”

Section: Evaluation Of Prophylactic or Repository Activities Of Extractmentioning

confidence: 99%

Antipyretic and antimalarial activities ofSolenostemon monostachyus

Okokon

Davis

Azare

2015

Pharmaceutical Biology

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Context: Solenostemon monostachyus P. Beauv (Lamiaceae) is an important herb used traditionally in the treatment of malaria, fever, and other diseases. Objectives: Antiplasmodial and antipyretic activities of S. monostachyus aerial extract were evaluated to ascertain the folkloric claim of its antimalarial and antipyretic activities. Materials and methods: The extract (75-225 mg/kg) and fractions (chloroform and aqueous; 150 mg/kg) of S. monostachyus were investigated for suppressive, prophylactic, and curative antiplasmodial activities against chloroquine-sensitive Plasmodium berghei infections in Swiss albino mice and for antipyretic activity against 2,4-dinitrophenol and yeast-induced pyrexia. Artesunate (5 mg/kg) and pyrimethamine (1.2 mg/kg) were used as positive controls for antiplasmodial models. Thin films made from tail blood of each mouse were used to assess the level of parasitaemia of the mice.

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Section: Evaluation Of Prophylactic or Repository Activities Of Extractmentioning

confidence: 99%

Antipyretic and antimalarial activities ofSolenostemon monostachyus

Okokon

Davis

Azare

2015

Pharmaceutical Biology

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“…A method described by Peters (1965) was followed to assess the preventive activity. Five groups of mice (six mice in each group) were administered orally with 150, 250, and 350 mg/kg/d doses of AREBA and 1.2 mg/kg/d pyrimethamine (positive control) and 0.2 mL/mouse/d distilled water (negative control) for 4 consecutive days (D0-D3), respectively.…”

Section: Evaluation Of Preventive Activitymentioning

confidence: 99%

Antiplasmodial activity of aqueous extract ofBerberis aristataroots againstPlasmodium berghei-infected BALB/c mice

Chandel

Bagai

Semwal

et al. 2015

Pharmaceutical Biology

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Context: The rising problem of resistance to present antimalarial drugs stresses the need to look for newer antiplasmodial components with effective modes of action. The roots of Berberis aristata DC. (Berberidaceae) are used in the traditional medicine for malaria in various parts of India. Objective: The objective of this study was to evaluate antiplasmodial activity of B. aristata roots extract for the validation of its traditional medicinal use. Material and methods: Aqueous root extract of Berberis aristata (AREBA) was screened for its in vitro as well as in vivo antiplasmodial activity against lethal rodent malaria parasite Plasmodium berghei NK65. In vitro activity was evaluated against schizont maturation of P. berghei using various concentrations ranging from 1 to 100 mg/mL. For in vivo studies, AREBA at the doses of 150, 250, 350, and 650 mg/kg/d was administered to P. berghei infected BALB/c mice orally for 4 consecutive days (D0-D3). Results: AREBA showed in vitro antiplasmodial activity with an IC 50 value of 40 mg/mL. In vivo studies demonstrated a variable dose-dependent chemosuppression with higher efficacy at lower doses. At a dose of 350 mg/kg/d, the suppressive and preventive activities were found to be 67.1% and 53.9%, respectively, followed by enhancing mean survival period up to 12.8 d for the curative assay versus 7.5 d for the untreated mice. Discussion and conclusion: These results provide relevant scientific evidences for the traditional medicinal use of this plant as malaria remedy and further advocates the isolation and characterization of active antiplasmodial principle from this plant.

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“…The repository activity of the extract and Coartem® (Artemether/Lumefantrine) was assessed by using the method described by Peters (1965). The mice were randomly divided into four groups of five mice each.…”

Section: Evaluation Of Prophylactic Activitiesmentioning

confidence: 99%

Neurohistological and immunohistochemical effects of prophylactic ethanolic leaf extract of Nauclea latifolia and Artemether/Lumefantrine on the hippocampus of Plasmodium berghei-infected mice

Edagha¹,

Atting²,

Ekanemesang³

2018

Afr. J. Pharm. Pharmacol.

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The prophylactic effect of ethanolic leaf extract of Nauclea latifolia, widely used in herbal malarial treatment, was compared against standard drug Coartem® (Artemether/Lumefantrine) in Plasmodiuminfected mice was investigated, and thereafter analysed for organosomatic index, parasitemia, histomorphological and immunohistochemical changes in the hippocampus. Twenty male mice about 6 to 8 weeks weighing 20 to 24 g were alloted into four groups of five mice each. Group 1 served as control received placebo; group 2 received extract 500 mg/kg; group 3 received extract 1000 mg/kg; and group 4 received 5 mg/kg of Artemether/Lumefantrine. Extract and drug administrations were performed for 3 days, and thereafter mice were infected with 10 6 of Plasmodium berghei parasites, and monitored for 4 days, after which the experiment was terminated. Thick blood smear were prepared from lateral tail vein, then under anesthesia via a cocktail of xylaxine and ketamine, intracardiac perfusion was performed first with phosphate buffered saline to clear systemic blood, and then 4% paraformaldehyde to fixed brain for routine histology and immunohistochemistry. Result indicates organosomatic index was not statistically significant, parasitemia in the treated groups were significantly (p<0.05) decreased compared to control and were corroborated in the photomicrographs of respective blood morphology, however, histologically there was moderate to severe distortion of the hippocampus across the groups, but glial fibrillary acidic protein expression, a marker for neurotoxicity indicated that group 4 had the most immunolabelling intensity compared to other test groups. In conclusion, the prophylactic ethanolic extract of N. latifolia and Artemether/Lumefantrine cleared parasitemia also seen in the blood morphology, and low dose N. latifolia plausibly has better safety and hippocampal toxicity profile with decreased neuronal shrinkage and distortions, with a more down regulated glial fibrillary acid protein than higher extract doses and Artemether/lumefantrine.

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Drug resistance in Plasmodium berghei. I. Chloroquine resistance

Cited by 316 publications

References 4 publications

Antipyretic and antimalarial activities ofSolenostemon monostachyus

Antipyretic and antimalarial activities ofSolenostemon monostachyus

Antiplasmodial activity of aqueous extract ofBerberis aristataroots againstPlasmodium berghei-infected BALB/c mice

Neurohistological and immunohistochemical effects of prophylactic ethanolic leaf extract of Nauclea latifolia and Artemether/Lumefantrine on the hippocampus of Plasmodium berghei-infected mice

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