Drug resistance and molecular epidemiology of Mycobacterium tuberculosis in Mexico: A systematic review

Flores-Treviño, Samantha; Mendoza-Olazarán, Soraya; Garza‐González, Elvira

doi:10.21149/spm.v56i1.7324

Cited by 22 publications

(25 citation statements)

References 30 publications

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“…The most predominant clades found in our study were the ill-defined T clade (SIT53), the Haarlem clade (SIT50), the Latin American-Mediterranean clade (SIT42) and the IS 6110 -low-banding X clade (SIT137). These results are similar to those reported in several studies from across the country [ 8 – 17 ]. And considering that SIT42 evolved originally from SIT53, this group is very dominant in this region [ 27 ].…”

Section: Discussionsupporting

confidence: 92%

“…Although a high number of previously reported spoligotypes were identified in Mexico [ 12 , 13 , 17 ], 19.1% of our isolates showed SIT (335, 371, 406, 469, 1231, 1686, 2007, 2212 and 3434) which were previously unreported in the country.…”

Section: Discussionmentioning

confidence: 50%

“…Several studies have reported the genetic diversity of M . tuberculosis strains in Mexico, but little information is available from the state of Jalisco [ 8 – 17 ]. Previously we evaluated phenotypic and genotypic resistance of M .…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Mycobacterium tuberculosis from Guadalajara, Mexico and Identification of a Rare Multidrug Resistant Beijing Genotype

et al. 2015

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Determining the genetic diversity of M. tuberculosis strains allows identification of the distinct Mycobacterium tuberculosis genotypes responsible for tuberculosis in different regions. Several studies have reported the genetic diversity of M. tuberculosis strains in Mexico, but little information is available from the state of Jalisco. Therefore, the aim of this study was to determine the genetic diversity of Mycobacterium tuberculosis clinical isolates from Western Mexico. Sixty-eight M. tuberculosis isolates were tested for susceptibility to first-line drugs using manual Mycobacteria Growth Indicator Tube method and genotyped using spoligotyping and IS6110-restriction fragment length polymorphism (RFLP) pattern analyses. Forty-seven (69.1%) isolates were grouped into 10 clusters and 21 isolates displayed single patterns by spoligotyping. Three of the 21 single patterns corresponded to orphan patterns in the SITVITWEB database, and 1 new type that contained 2 isolates was created. The most prevalent lineages were T (38.2%), Haarlem (17.7%), LAM (17.7%), X (7.4%), S (5.9%), EAI (1.5%) and Beijing (1.5%). Six (12.8%) of the clustered isolates were MDR, and type 406 of the Beijing family was among the MDR isolates. Seventeen (26.2%) isolates were grouped into 8 clusters and 48 isolates displayed single patterns by IS6110-RFLP. Combination of IS6110-RFLP and spoligotyping reduced the clustering rate to 20.0%. The results show that T, Haarlem, and LAM are predominant lineages among clinical isolates of M. tuberculosis in Guadalajara, Mexico. Clustering rates indicated low transmission of MDR strains. We detected a rare Beijing genotype, SIT406, which was a highly resistant strain. This is the first report of this Beijing genotype in Latin America.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 50%

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Genetic Diversity of Mycobacterium tuberculosis from Guadalajara, Mexico and Identification of a Rare Multidrug Resistant Beijing Genotype

et al. 2015

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“…We identified 32 SITs, of which 17 (53%) (1, 2, 19, 20, 42, 46, 49, 50, 51, 53, 92, 119, 291, 334, 948, 787 and 2212) had been previously described in DR-TB and MDR-TB isolates circulating in Mexico [ 18 , 19 ], and other Latin American countries [ 40 , 41 , 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of drug and multidrug-resistant Mycobacterium tuberculosis circulating in Veracruz, Mexico

et al. 2018

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BackgroundMexico is one of the most important contributors of drug and multidrug-resistant tuberculosis in Latin America; however, knowledge of the genetic diversity of drug-resistant tuberculosis isolates is limited.MethodsIn this study, the genetic structure of 112 Mycobacterium tuberculosis strains from the southeastern Mexico was determined by spoligotyping and 24-loci MIRU-VNTRs.FindingsThe results show eight major lineages, the most of which was T1 (24%), followed by LAM (16%) and H (15%). A total of 29 (25%) isolates were identified as orphan. The most abundant SITs were SIT53/T1 and SIT42/LAM9 with 10 isolates each and SIT50/H3 with eight isolates. Fifty-two spoligotype patterns, twenty-seven clusters and ten clonal complexes were observed, demonstrating an important genetic diversity of drug and multidrug-resistant tuberculosis isolates in circulation and transmission level of these aggravated forms of tuberculosis. Being defined as orphan or as part of an orphan cluster, was a risk factor for multidrug resistant-tuberculosis (OR 2.5, IC 1.05–5.86 and OR 3.3, IC 1–11.03, respectively). Multiple correspondence analyses showed association of some clusters and SITs with specific geographical locations.ConclusionsOur study provides one of the most detailed description of the genetic structure of drug and multidrug-resistant tuberculosis strains in southeast Mexico, establishing for the first time a baseline of the genotypes observed in resistant isolates circulating, however further studies are required to better elucidate the genetic structure of tuberculosis in region and the factors that could be participating in their dispersion.

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“…On the other hand, the control sample set of 58 PTB DNAs, showed that 26 (44.8%) strains, which included 84.6% Beijing, 11.5% T1 and 3.8% T2 genotypes, were resistant to at least 1 drug. There were 10 strains (17.2%), 9 Beijing and 1 T1 that were of MDR type, resistant to at least INH (Ser 315 Thr in KatG) and RIF (Ser 531 Leu in RpoB), the 2 most powerful anti-TB drugs (33). The proportion of MDR-TB in the Beijing vs. non-Beijing genotype PTB strains was statistically different (p < 0.02).…”

Section: Molecular Screens For Drug Resistancementioning

confidence: 97%

Molecular Characteristics of Mycobacterium tuberculosis Strains Isolated from Cutaneous Tuberculosis Patients in China

Jiang

Jin²,

Vissa³

et al. 2017

Acta Derm Venerol

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Cutaneous tuberculosis (CTB) is probably underreported due to difficulties in detection and diagnosis. To address this issue, genotypes of Mycobacterium tuberculosis strains isolated from 30 patients with CTB were mapped at multiple loci, namely, RD105 deletions, spacer oligonucleotides, and Mycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeats (MIRU-VNTRs). Fifty-eight strains of pulmonary tuberculosis (PTB) were mapped as experimental controls. Drug resistance-associated gene mutations were determined by amplicon sequencing of target regions within 7 genes. Beijing family isolates were the most prevalent strains in CTB and PTB. MIRU-VNTR typing separated the Beijing strains from the non-Beijing strains, and the majority of CTB could be separated from PTB counterparts. Drug resistance determining regions showed only one CTB strain expressing isomazid resistance. Thus, while the CTB strains belonged to the same phylogenetic lineages and sub-lineages as the PTB strains, they differed at the level of several MIRU-VNTRs and in the proportion of drug resistance.

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Drug resistance and molecular epidemiology of Mycobacterium tuberculosis in Mexico: A systematic review

Cited by 22 publications

References 30 publications

Genetic Diversity of Mycobacterium tuberculosis from Guadalajara, Mexico and Identification of a Rare Multidrug Resistant Beijing Genotype

Genetic Diversity of Mycobacterium tuberculosis from Guadalajara, Mexico and Identification of a Rare Multidrug Resistant Beijing Genotype

Genetic diversity of drug and multidrug-resistant Mycobacterium tuberculosis circulating in Veracruz, Mexico

Molecular Characteristics of Mycobacterium tuberculosis Strains Isolated from Cutaneous Tuberculosis Patients in China

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