Drug release kinetics and biological properties of a novel local drug carrier system

Abstract

Background: The purpose of this in vitro study was to investigate drug release kinetics and cytotoxicity of a novel drug delivery system for treatment of periodontitis. Materials and Methods: This in vitro study addresses the fabrication of a polycaprolactone/alginic acid-based polymeric film loaded with metronidazole, as a basic drug in the treatment of periodontal diseases. Films were prepared by solvent casting technique. Four formulations with different percentages of drug by weight (3%, 5%, 9%, and 13%) were prepared. Drug release kinetics were investigated using ultraviolet–visible spectroscopy during (one week). Data were analyzed using repeated measures ANOVA. Cytotoxicity of drug-loaded system extracts was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using L929 cells after 24-h incubation. The results were evaluated according to ISO standard 10993-5 and assessed using ANOVA and Tukey's tests at a significance level of P < 0.05. Results: All polymeric films showed a burst drug release followed by a gradual release. Drug release data were fitted well with the first-order kinetic model in all drug-containing formulations indicating that drug release is a fraction of remaining drug in the matrix. Drug release is mainly driven by diffusion of medium into the composite matrix. 3%wt metronidazole-containing formulation exhibited the best MTT result. Conclusion: The findings of this study supported the synthesis of drug-loaded periodontal films with 3% metronidazole due to better biological properties along with the ability of acceptable drug release to eradicate anaerobic periodontal bacteria.

Levofloxacin HCl-Incorporated Zein-Based Solvent Removal Phase Inversion In Situ Forming Gel for Periodontitis Treatment

“…Metronidazole-loaded polycaprolactone/alginic acid-based polymeric film in treating periodontal diseases showed a burst drug release followed by a gradual release. It fitted well with the first-order kinetic model indicating that drug release is a fraction of the drug remaining in the matrix [65]. When liquid diffusion and polymer relaxation rates are of the same magnitude during immersion in the release medium, anomalous or non-Fickian diffusion is dominant [66].…”

Pharmacokinetic Interpretation of Applying Local Drug Delivery System for the Treatment of Deep Surgical Site Infection in the Spine

“…They play a crucial role in developing pharmaceutical formulations, assessing drug release mechanisms in both laboratory and real-world settings, and essentially in formulating the most effective strategies for innovative systems [ 20 ]. Creating mathematical models to understand how drugs are released over time is crucial for studying the processes that control this release [ 21 ]. Many comprehensive reviews have been written about mathematical modeling in different drug delivery systems, such as biodegradable polymeric systems [ 22 ], dissolution-controlled drug delivery systems [ 23 ], microsphere delivery systems [ 24 ], and hydrogel networks [ 25 ].…”

Prodigiosin/celecoxib-loaded into zein/sodium caseinate nanoparticles as a potential therapy for triple negative breast cancer

“…The good linearity obtained from the results could be attributed to dependency of drug release rate on the concentration of drug within the core of the NPs, which means that the amount of released drug will decrease by reducing its concentration. In other words, release media will diffuse through matrix and forces drug to be released 70 .…”