Drug-Related Mutational Patterns in Hepatitis B Virus (HBV) Reverse Transcriptase Proteins From Iranian Treatment-Naïve Chronic HBV Patients

Mahabadi, Mostafa; Norouzi, Mehdi; Alavian, S M; Samimirad, Katayoon; Azad, Talat Mokhtari; Saberfar, Esmaeil; Mahmoodi, Mahmood; Ramezani, Fatemeh; Karimzadeh, Hadi; Malekzadeh, Reza; Montazeri, Ghodratollah; Nejatizadeh, Azim; Ziaee, Masood; Abedi, Farshid; Ataei, Behrooz; Yaran, Majid; Sayad, Babak; Somi, Mohammad Hossein; Sarizadeh, Gholamreza; Sanei-Moghaddam, Ismaeil; Ghanaei, F Mansour; Rafatpanah, Houshang; Pourhosseingholi, Mohammad Amin; Keyvani, H; Kalantari, Ebrahim; Saberifiroozi, Mehdi; Judaki, Mohammad Ali; Ghamari, Shiva; Daram, Maryam; Fazeli, Zeinab; Goodarzi, Zahra; Khedive, Abolfazl; Moradi, Abdolvahab; Jazayeri, Seyyed Mohammad

doi:10.5812/hepatmon.6712

Cited by 22 publications

(24 citation statements)

References 44 publications

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“…The rare S78T mutation also observed in one patient can lead to tenofovir resistance (45). Other mutation that observed in this study was L91I which reported as an Entecavir, lamivudine -related resistance mutation (46, 47). …”

Section: Discussionmentioning

confidence: 49%

Genotyping and Mutation Pattern in the Overlapping MHR Region of HBV Isolates in Southern Khorasan, Eastern Iran

et al. 2016

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BackgroundHepatitis B virus, with 8 known distinct genotypes, is one of the most serious health problems which results to liver injuries. The surface gene of Hepatitis B virus completely overlaps with the polymerase gene. Mutations in the RT gene result in changes in the overlapping hepatitis B surface antigen.ObjectivesThe present study aimed to evaluate the genotypes and prevalence of mutations in a segment of S and RT gene in HBV isolates in Southern Khorasan, Iran.MethodsThis was a population-based study comprising 5,235 randomized samples for HBV screening. A nested-polymerase chain reaction (PCR) test was followed by direct sequencing, and the sequences blast with present sequences of NCBI database for genotyping. Alignment and phylogenic analysis was performed using MEGA-6 software, and mutation pattern of this segment was finally surveyed in Bioedit software.ResultsThe mean age was 39.07 ± 14.04 years, with 52.2% female and 47.8% male. All isolates belonged to HBV genotype D, sub-genotype D1. The most amino acid substitutions of surface protein were Q129H (34.42%) and A168V (8.2%), other escape mutants observed in this study were P127L/T, S117G, T125M, S143L, D144E and E164D. In the RT gene, Q149K was the most frequently identified amino acid substitution (9.83%), followed by L122F (8.19%), N118D/T (6.55%), L157M (4.91%), and H124Y (3.27%).ConclusionsThis finding represents an ongoing dominancy of HBV genotype D in Eastern Iran, corresponding to other parts of Iran. There were a lot of variations in the S gene leading to an escape mutation, some of which affected the corresponding area of the RT region.

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Section: Discussionmentioning

confidence: 49%

Genotyping and Mutation Pattern in the Overlapping MHR Region of HBV Isolates in Southern Khorasan, Eastern Iran

et al. 2016

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“…The truncated S protein that results from this stop codon may have a transactivating effect on several proteins in host cells [Lai and Yeh, ; Yeh, ]. There are discrepancies in the surveys regarding the antiviral resistant variants among treatment‐naïve Iranian HBV carriers in different studies [Amini‐Bavil‐Olyaee et al, ; Mahabadi et al, ]. Although two strains carried a stop codon at HBsAg, HBsAg was still detectable in those two cases.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of hepatitis B virus (HBV) strains circulating in the northern coast of the Persian Gulf and its comparison with worldwide distribution of HBV subgenotype D1

Pourkarim

Vergote

Amini-Bavil-Olyaee

et al. 2014

Journal of Medical Virology

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Iran is a large country that covers the northern coast of the Persian Gulf. Iranian residents of this coastal region interact closely with people from neighboring countries because of historical and cultural relationships, as well as economic activities. In addition, the inhabitants of this border region have experienced several wars, which have affected public health infrastructures. This study characterized for the first time, the evolution of the full-length genome of HBV strains in asymptomatic carrier patients living in this particular region. In addition, this study was compared and complemented by a comprehensive evolutionary analysis of the worldwide geographical distribution of HBV subgenotype D1. Evolutionary analysis demonstrates that patients living in the northern coast of the Persian Gulf are mainly infected with HBV subgenotype D1, subtype ayw2. Specific mutations related to advanced liver disease were found more frequently in these strains compared to other strains isolated from asymptomatic carriers from other regions of Iran. This global comprehensive analysis showed that HBV subgenotype D1 strains have a worldwide distribution and that human mobility and immigration had a large impact on dispersal of HBV subgenotype D1, subtype ayw2 in Middle Eastern countries such as Iran, Syria, and Turkey. In addition to association of subtype ayw2 with subgenotype D1, it was demonstrated that other HBV subtypes like adw2, ayw1, and ayw3 are associated with HBV subgenotype D1 in different regions of the world. This study also revealed a remarkable distribution of subgenotype D1, subtype ayw4 although this particular subtype is associated with subgenotype D4 of HBV in European countries.

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“…Their results showed that in comparison with other studies, the mutations related to drug resistance in Iranian treatmentnaïve chronic HBV patients were higher. Hence, in order to attain an excellent regimen option, they suggested that chronic HBV patients should be monitored closely before the start of therapy (31).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Hepatitis B Surface Antigen Among People Attending Medical Diagnostic Laboratories in Neyshabur, Northeast of Iran, During Years 2011 to 2015

Salehi

Najafi

2017

Arch Pediatr Infect Dis

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Background: Hepatitis B Virus (HBV) infection remains a public health and health care issue in Iran. Since the data on its outbreak is required for effectual policies, this epidemiological study was performed on population from Neyshabur, where there has been no former epidemiological information. Objectives: This study aimed at investigating the epidemiology of hepatitis B surface antigen among subjects from Neyshabur, from year 2011 to 2015. Methods: The researchers established this study from data collected from a surveillance system. Screening tests were carried out by the Chemioluminsance method. Age, gender, referral year and month were the investigated variables. Data were analyzed by the SPSS-20 software. Results: The prevalence of HBsAg in Neyshabur was 4.11%. In the age group of more than 40 years (1.87%) and females (2.2%) had significantly (P < 0.0001) higher rates of HBV seropositivity. Also the year 2011 (5.26%) and the month of July (0.48%) had the highest prevalence. Conclusions: Hepatitis B Virus (HBV) infection rate was seemingly high in Neyshabur, even higher than other parts of Iran. An immunization program should be continued progressively for high-risk areas, especially the Neyshabur city of Iran.

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Drug-Related Mutational Patterns in Hepatitis B Virus (HBV) Reverse Transcriptase Proteins From Iranian Treatment-Naïve Chronic HBV Patients

Cited by 22 publications

References 44 publications

Genotyping and Mutation Pattern in the Overlapping MHR Region of HBV Isolates in Southern Khorasan, Eastern Iran

Genotyping and Mutation Pattern in the Overlapping MHR Region of HBV Isolates in Southern Khorasan, Eastern Iran

Molecular characterization of hepatitis B virus (HBV) strains circulating in the northern coast of the Persian Gulf and its comparison with worldwide distribution of HBV subgenotype D1

Prevalence of Hepatitis B Surface Antigen Among People Attending Medical Diagnostic Laboratories in Neyshabur, Northeast of Iran, During Years 2011 to 2015

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