Drug Ototoxicity

Hawkins, Joseph E.

doi:10.1007/978-3-642-66082-5_16

Cited by 79 publications

(40 citation statements)

References 125 publications

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“…However, such changes are unlikely to account for the rapid onset and offset of hearing changes noted in this study. Vascular alterations, notably vasoconstriction in the stria vascularis, suprastrial ligament and basilar membrane or a direct pharmacological effect on the hair cells may be more relevant (Hawkins, 1976;Koegel, 1985) to rapid reversible changes in hearing. The mechanism of hearing impairment may be similar to that associated with salicylism.…”

Section: Discussionmentioning

confidence: 99%

Quinine induces reversible high‐tone hearing loss.

Roche

Silamut

Pukrittayakamee

et al. 1990

Brit J Clinical Pharma

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Section: Discussionmentioning

confidence: 99%

Quinine induces reversible high‐tone hearing loss.

Roche

Silamut

Pukrittayakamee

et al. 1990

Brit J Clinical Pharma

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“…2). The position in which this chinchilla outer hair cell was photographed allows visualization of the region of the 'fonticulus', the apical portion of the cell which is not covered by the cuticular plate (Hawkins, 1976). The nucleus is in its normal position near the base of the cell.…”

Section: Morphologymentioning

confidence: 99%

Comparison of isolated outer hair cells from five mammalian species

Zajic

Schacht

1987

Hearing Research

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Live outer hair cells were isolated from guinea pig, chinchilla, rat, mouse, and gerbil. The organ of Corti from selected turns of the cochlea was briefly incubated with collagenase and outer hair cells were separated from the tissue by micromanipulation under microscopic observation.Morphological criteria for cell viability were: (1) cylindrical cell shape without swelling or distortion of the membrane;(2) location of the nucleus in its normal position near the base of the cell; (3) cytoplasm devoid of Brownian motion and granulation.Both yield and quality (as judged by these morphological criteria) of isolated hair cells varied with the species and the turn from which the isolation was attempted. Consistently high yields and cells of good morphology were obtained from guinea pigs and chinchillas.Fewer cells were obtained from rats and mice, and their quality was less consistent. Gerbils gave the poorest yield and quality of outer hair cells. In all species, the preparation was more successful from the apical than from the basal turn. The length of apical hair cells varied almost 4-fold from 60 to 80 pm in guinea pig and chinchilla to 20 to 40 pm in the other species, while their diameter only varied 1.5-fold from 7 (mouse) to 10 pm (chinchilla). Outer hair cells could be maintained in vitro in good condition for several hours. Typical early signs of degeneration were increased Brownian motion and granulation in the cytoplasm, upward movement of the nucleus, or distortion of cell shape. Degeneration was always accompanied by a shortening along the long axis.

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“…Microtubules can also fan out from the basal body, which resides in the fonticulus-a region in the CP devoid of F-actin (Hawkins, 1976;Raphael et al, 1994;Raphael and Altschuler, 2003;Chacon-Heszele et al, 2012). The ends of the columnar microtubules are coated with a fuzzy cap of electrondense material and extend deep into the CP (Jaeger et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

ACF7 Is a Hair-Bundle Antecedent, Positioned to Integrate Cuticular Plate Actin and Somatic Tubulin

Antonellis¹,

Pollock²,

Chou³

et al. 2013

J. Neurosci.

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The precise morphology of the mechanosensitive hair bundle requires seamless integration of actin and microtubule networks. Here, we identify Acf7a (actin crosslinking family protein 7a) as a protein positioned to bridge these distinct cytoskeletal networks in hair cells. By imaging Acf7a-Citrine fusion protein in zebrafish and immunolabeling of vestibular and cochlear mouse hair cells, we show that Acf7a and ACF7 circumscribe, underlie, and are interwoven into the cuticular plate (CP), and they also encircle the basal body of the kinocilium. In cochlear hair cells, ACF7 localization is graded, with the highest concentration near each fonticulus-an area free of F-actin in the region of the CP that contains the basal body. During hair-cell development and regeneration, Acf7a precedes formation of the hair bundle and CP. Finally, electron tomography demonstrates that the ends of microtubules insert into the CP and are decorated with filamentous linkers connecting microtubules to the CP. These observations are consistent with ACF7 being a linker protein, which may shape the cytoskeleton of the hair cell early during hair-bundle genesis.

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Drug Ototoxicity

Cited by 79 publications

References 125 publications

Quinine induces reversible high‐tone hearing loss.

Quinine induces reversible high‐tone hearing loss.

Comparison of isolated outer hair cells from five mammalian species

ACF7 Is a Hair-Bundle Antecedent, Positioned to Integrate Cuticular Plate Actin and Somatic Tubulin

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