Drug orientations within statin-loaded lipoprotein nanoparticles by <sup>19</sup>F solid-state NMR

Lau, Sophie; Stanhope, Naomi E.; Griffin, John M.; Hughes, Eleri; Middleton, David A.

doi:10.1039/c9cc05344a

Cited by 6 publications

(2 citation statements)

References 20 publications

(30 reference statements)

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“…Furthermore, the chemical shift dispersion (but also the chemical shift anisotropy) is much larger than that of proton, leading to less signal overlap. It can provide information also on the molecular orientation inside a DDS [ 33 ].…”

Section: 1 H-x Nmrmentioning

confidence: 99%

Solid-State NMR Spectroscopy: A Key Tool to Unravel the Supramolecular Structure of Drug Delivery Systems

Porcino

Gref

et al. 2021

Molecules

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In the past decades, nanosized drug delivery systems (DDS) have been extensively developed and studied as a promising way to improve the performance of a drug and reduce its undesirable side effects. DDSs are usually very complex supramolecular assemblies made of a core that contains the active substance(s) and ensures a controlled release, which is surrounded by a corona that stabilizes the particles and ensures the delivery to the targeted cells. To optimize the design of engineered DDSs, it is essential to gain a comprehensive understanding of these core–shell assemblies at the atomic level. In this review, we illustrate how solid-state nuclear magnetic resonance (ssNMR) spectroscopy has become an essential tool in DDS design.

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Section: 1 H-x Nmrmentioning

confidence: 99%

Solid-State NMR Spectroscopy: A Key Tool to Unravel the Supramolecular Structure of Drug Delivery Systems

Porcino

Gref

et al. 2021

Molecules

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“…Solid-state NMR has been used extensively to characterize nanoparticle–ligand interactions. − Recently, several novel solution-state NMR methods have been used to gain information on these kinds of interactions as well. ,− Comprehensive multiphase (CMP) NMR, on the other hand, is the only method that can simultaneously probe solid, liquid, and gel-like phases in an intact sample . A CMP-NMR probe contains the ability to handle the high RF power required for solid-state NMR, as well as a lock, pulse field gradient, and susceptibility matching required for both solution and gel-state NMR.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Multiphase NMR Examination of Amino Acids Binding to the Dynamic Shell of Polystyrene Nanoparticles to Understand Environmental Hazards Associated with Nanoscale Plastic

Biswas

Soong

Schmidig

et al. 2022

ACS Appl. Nano Mater.

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Plastic pollution is a growing environmental concern. In addition to posing hazards to wildlife, micro- and nano-scale plastic particles can adsorb toxic small molecules from polluted waterways, which may be released when these plastic particles are ingested by animals or humans. Previous NMR studies have examined the binding between surface-modified polystyrene nanoparticles and amino acids as models for small molecules with a variety of functional groups. These previous studies, however, could only examine the liquid phase and therefore focused on the small molecules. In the current study, we use comprehensive multiphase NMR (CMP-NMR) to examine both the small molecules and polystyrene nanoparticles in all phases including liquid, solid, and gel-like phases. Through proton spectral editing techniques and 13C solid-state NMR experiments, we find that the polystyrene nanoparticles contain both solid and gel-like fractions. The bound amino acid exists primarily in the gel-like phase, with very little amino acid existing in the true solid phase. This suggests that the bound amino acid interacts with the nanoparticle shell rather than the solid-like core. These experiments relied on the ability of CMP-NMR to separately observe the solid, liquid, and gel-like phases of the same sample and demonstrate the complementary nature of this approach for understanding complex multiphase systems.

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¹⁹F Solid‐State NMR and Vibrational Raman Characterization of Corticosteroid Drug‐Lipid Membrane Interactions

et al. 2021

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Drug interactions with phospholipid bilayers underpin their behaviour in cell membranes and in liposomal delivery formulations. Liposomal drug delivery in ocular medicine can overcome the physical barriers of the eye and better enable the active molecule to reach its target. Here, Raman and 19F solid‐state NMR spectroscopy are used to characterise the interactions of two ocular corticosteroid drugs, difluprednate (DFP) and fluorometholone (FML), with multilamellar vesicles of phosphatidylcholine (PC). 31P NMR confirms that the lipid bilayer tolerates a high drug concentration (a drug: lipid molar ratio of 1 : 10). The 19F NMR spectra of the drugs in lipid bilayers reveal that FML and DFP have different average orientations within the lipid bilayer. Raman spectra of dried lipid films reveal that PC separates from DFP but not from FML, the less lipophilic of the two drugs. This combined approach will assist the design of, and inform the development of, improved liposomal preparations.

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Drug orientations within statin-loaded lipoprotein nanoparticles by ¹⁹F solid-state NMR

Abstract: NMR measurements of 19F chemical shift anisotropy and 1H–19F dipolar couplings provide novel information on the molecular orientation of fluorine-containing statin drugs in rHDL lipoprotein nanoparticles, a drug delivery system under investigation.

Cited by 6 publications

References 20 publications

Solid-State NMR Spectroscopy: A Key Tool to Unravel the Supramolecular Structure of Drug Delivery Systems

Solid-State NMR Spectroscopy: A Key Tool to Unravel the Supramolecular Structure of Drug Delivery Systems

Comprehensive Multiphase NMR Examination of Amino Acids Binding to the Dynamic Shell of Polystyrene Nanoparticles to Understand Environmental Hazards Associated with Nanoscale Plastic

¹⁹F Solid‐State NMR and Vibrational Raman Characterization of Corticosteroid Drug‐Lipid Membrane Interactions

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Drug orientations within statin-loaded lipoprotein nanoparticles by 19F solid-state NMR

Abstract: NMR measurements of 19F chemical shift anisotropy and 1H–19F dipolar couplings provide novel information on the molecular orientation of fluorine-containing statin drugs in rHDL lipoprotein nanoparticles, a drug delivery system under investigation.

Cited by 6 publications

References 20 publications

Solid-State NMR Spectroscopy: A Key Tool to Unravel the Supramolecular Structure of Drug Delivery Systems

Solid-State NMR Spectroscopy: A Key Tool to Unravel the Supramolecular Structure of Drug Delivery Systems

Comprehensive Multiphase NMR Examination of Amino Acids Binding to the Dynamic Shell of Polystyrene Nanoparticles to Understand Environmental Hazards Associated with Nanoscale Plastic

19F Solid‐State NMR and Vibrational Raman Characterization of Corticosteroid Drug‐Lipid Membrane Interactions

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Drug orientations within statin-loaded lipoprotein nanoparticles by ¹⁹F solid-state NMR

¹⁹F Solid‐State NMR and Vibrational Raman Characterization of Corticosteroid Drug‐Lipid Membrane Interactions