Drug‐induced Changes in the Concentration of 5‐or Indolyl Compounds in Cerebrospinal Fluid and Caudate Nucleus

Abstract

The effects of reserpine and imipramine on the concentration of indolyl compounds bearing an OR group in position 5 (5-OR indolyl compounds) in the cerebrospinal fluid and the caudate nucleus of the dog have been studied. Reserpine was shown to produce an increase in the concentration of these compounds in the cerebrospinal fluid. In the caudate nucleus reserpine produced a fall in the concentration of the basic 5-OR indolyl compounds accompanied by an equivalent increase in the concentration of acidic 5-OR indolyl compounds. No significant change in the concentration of 5-OR indolyl compounds in the cerebrospinal fluid was observed after treatment with imipramine.In a previous publication (Ashcroft & Sharman, 1960) it was reported that there was a difference in the concentration of 5-hydroxyindolyl compounds in the cerebrospinal fluid of patients suffering from depressive psychoses and patients with neurological diseases. The present investigation on dogs was carried out to test the possibility that changes in the metabolism of 5-hydroxytryptamine in the body might be reflected in changes in the cerebrospinal fluid level of indolyl compounds bearing an OR group in position 5. Sharman (1960) has shown that many indolyl compounds bearing an OR group in position 5, where R is a hydrogen atom, or an aryl or alkyl group, and unsubstituted in the 2 position of the indole nucleus, show the fluorescence in 3N hydrochloric acid attributed to 5-hydroxyindolyl compounds by Udenfriend, Bogdanski & Weissbach (1955) and demonstrated for N-acetyl-5-methoxytryptamine by Axelrod & Weissbach (1961). It is possible that traces of indolyl compounds bearing an OR group in position 5 other than 5-hydroxytryptamine or 5-hydroxyindol-3-ylacetic acid are present in the cerebrospinal fluid.

UPTAKE OF 5‐HYDROXY[14C]TRYPTOPHAN BY RAT AND DOG BRAIN SLICES

“…The dose needed for 50% inhibition was read off by interpolation. brain slices were homogenized in two volumes (w/v) of 0.1 N HCO and 5-OR indolyl compounds extracted as described by Ashcroft & Sharman (1962). In this method the total 5-OR indolyl compounds are measured in the supernatant obtained after removal of protein from the homogenates.…”

Influence of adrenergic receptor blockade on circulatory and metabolic effects of disordered neurotransmitter function in stroke patients.

“…15 The remaining portion was placed on Amberlite columns from which NE was eluted by 2/3 M boric acid and analyzed fluorometrically using the trihydroxyindole reaction according to modifications of the method of Renzini et al 16 and Valori et al 17 HVA and 5HIAA were organically extracted from CSF (usually 2.2 ml) using a modified combination of the methods of Ashcroft et al 18 and Gerbode and Bowers. 19 Both compounds were fluorometrically assayed, 5HIAA by the technique of Ashcroft and Sharman 20 and HVA according to Curzon et al 21 Cyclic AMP in CSF was analyzed by a protein binding assay similar to radioimmunoassay by a combination of the methods of Gilman 22 and of Brown et al 23 Glucose was measured in arterial and cerebral venous blood by an enzymatic spectrophotometric technique. 24 Lactate and pyruvate were measured by the enzymatic method of Rosenberg and Rush.…”

The Effect of Prenylamine on the Metabolism of Catechol Amines and 5‐hydroxytryptamine in Brain and Adrenal Medulla

“…Estimation of 5-hydroxyindolyl compounds. Basic and acidic compounds, corresponding mainly to 5-hydroxytryptamine and 5-hydroxyindolylacetic acid, were always analysed separately (Ashcroft & Sharman, 1962), theformer by extracting the deproteinized tissue extract atpH 10 with n-butanol (Bogdarski, Pletscher, Brodie & Udenfriend, 1956), the latter by extracting with ether in the presence of hydrochloric acid (pH 1 to 2) (Udenfriend, Titus & Weissbach, 1955). An Aminco-Bowman spectrophotofluorimeter was used for the estimations.…”