Drug efficacy on zoonotic nematodes of the Anisakidae family: new metabolic data

Abstract

In the Anisakidae family, there are nematodes, most of which are parasitic for important commercial fish species. Both public health risks and socio-economic problems are attributed to these parasites. Despite these concerns, knowledge of the metabolism of these parasites remains unknown. Therefore, the main objective of this study was to investigate the receptors of drugs and oxidative metabolic status of two Anisakidae species, Pseudoterranova decipiens (s. s.) and Contracaecum osculatum (s. s.), under the influence of anthelminthic drugs, ivermectin (IVM) and pyrantel (PYR), at different concentrations: 1.56, 3.125 and 6.25 μg mL−1 of culture medium for 3, 6, 9, 12 and 72 h. The mRNA expressions of the γ-aminobutyric acid receptor, acetylcholine receptor subunits, adenosine triphosphate-binding cassette transporters and antioxidative enzymes were determined. The total antioxidant capacity and glutathione S-transferase activity were also examined. To the best of the authors' knowledge, this is the first time that IVM and PYR have been tested against these parasitic nematodes.

Proteomic characterization of extracellular vesicles released by third stage larvae of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae)

“…In the last years, several studies have been carried out to investigate the biological mechanisms and molecular signals involved in the human accidental host infection and pathogenesis of Anisakis spp. ( Baird et al., 2016 ; Bušelić et al., 2018 ; Cavallero et al., 2018 ; Llorens et al., 2018 ; Harbar et al., 2019 ; Palomba et al., 2019 ; Łopieńska-Biernat et al., 2020 ; Marzano et al., 2020 ; Stryiński et al., 2020 ; Trumbić et al., 2021 ; Kochanowski et al., 2022 ; Polak et al., 2022 ). Nevertheless, studies focused to investigate molecules shaping the adaptation of these parasites to their natural hosts are rather limited ( Mehrdana and Buchmann, 2017 ; Palomba et al., 2020 ; Trumbić et al., 2021 ; Harbar et al., 2022 ).…”