“…For JNJ‐56136379, blood samples were collected predose and at 0.5, 1, 1.5, 2, 3, 4, 5, 6, 8, 10, 12, 24, 48, 72, 120, 216, 312, and 480 hours post dose, with analyses for JNJ‐56136379 plasma concentrations performed using liquid chromatography tandem mass spectrometry with an LLOQ of 10.0 ng/mL (PRA, Assen, the Netherlands). Details of the method are published elsewhere 19 . For plasma protein binding, the optimal test conditions, including time, temperature, pH, stability, and recovery, were tested previously in a separate in vitro study.…”