Drug-Based Gold Nanoparticles Overgrowth for Enhanced SPR Biosensing of Doxycycline

Abstract

In clinical chemistry, frequent monitoring of drug levels in patients has gained considerable importance because of the benefits of drug monitoring on human health, such as the avoidance of high risk of over dosage or increased therapeutic efficacy. In this work, we demonstrate that the drug doxycycline can act as an Au nanoparticle (doxy-AuNP) growth and capping agent to enhance the response of a surface plasmon resonance (SPR) biosensor for this drug. SPR analysis revealed the high sensitivity of doxy-AuNPs towards the detection of free doxycycline. More specifically, doxy-AuNPs bound with protease-activated receptor-1 (PAR-1) immobilized on the SPR sensing surface yield the response in SPR, which was enhanced following the addition of free doxy (analyte) to the solution of doxy-AuNPs. This biosensor allowed for doxycycline detection at concentrations as low as 7 pM. The study also examined the role of colloidal stability and growth of doxy-AuNPs in relation to the response-enhancement strategy based on doxy-AuNPs. Thus, the doxy-AuNPs-based SPR biosensor is an excellent platform for the detection of doxycycline and demonstrates a new biosensing scheme where the analyte can provide enhancement.

Minocycline-Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan-Induced Diabetic Mice

“…The antioxidant potential of minocycline, mino/AgNPs, and ascorbic acid were evaluated through DPPH free radical scavenging assay. Briefly, 100 µL of each minocycline, mino/AgNPs, and ascorbic acid at various concentrations (10,25,50, and 100 µg/mL) were added to the 2.9 mL of 0.1 mM DPPH solution in methanol. The resulting mixtures were kept in dark for 30 min.…”

“…Nanobiotechnology is among the demanding areas of research that make use of biological substances at nanoscale and find applications for them in different fields, such as biosensors [10], diagnostics [11], bio-imaging [12], catalysis [13], drug delivery systems [14], and nanomedicine [15]. In comparison to conventional drug formulations, nanomedicine has received more attention in the last few years due to its benefits, such as more precise diagnosis, a higher percentage of recovery, and more effective therapies [16].…”

High-Sensitivity PtSe2 Surface Plasmon Resonance Biosensor Based on Metal-Si-Metal Waveguide Structure

“…Surface plasmon resonance (SPR) found at the interface of two media (such as dielectric and metal) with opposite signs of dielectric constant can be excited by Otto or Kretschmann prism coupling model [ 1 , 2 ]. In recent years, SPR biosensors have been applied in food safety [ 3 , 4 ], environmental detection [ 5 ], medical diagnosis [ 6 , 7 , 8 ] and other fields, due to their outstanding performance of high detection accuracy, real time [ 9 , 10 ] and label free [ 11 ]. Traditional SPR biosensor structures usually contain glass prisms and noble metals which are in direct contact with biomolecules.…”

Minocycline Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan induced Diabetic Mice

“…Nanobiotechnology is among the demanding areas of research that make use of the biological substances at the nanoscale and find their applications in different fields such as biosensor (Kazmi et al, 2020), diagnostics (Hu et al, 2013), bio-imaging (Zhou et al, 2018), catalysis (Raza et al, 2017), drug delivery system (Kazmi et al, 2019) and nanomedicine (Wicki et al, 2015). In comparison to the conventional drug formulations, nanomedicine has retrieved more attention for the last few years due to its benefits such as more precise diagnosis, a higher percentage of recovery, and more effective therapies (Kouame et al, 2019).…”