Drug Assays on Organ Cultures of Biopsies from Human Tumours

Yarnell, Margaret M.; Ambrose, E. J.; Shepley, K.; Tchao, Ruy

doi:10.1136/bmj.2.5407.490

Cited by 20 publications

(1 citation statement)

References 7 publications

(2 reference statements)

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“…Very few papers (68) draw attention to the advantages of using human rather than experimental animal material or to the good mathematical planning of experiments. For example, at least three dose levels separated in geometric progression (61,64,(69)(70)(71), a principle long recognized (72) in whole animal techniques, are used.…”

Section: Methods Used I N Assessing the Beneficial A N D Adverse Effementioning

confidence: 99%

Tissue Culture in the Study of the Effects of Drugs

Dawson

Dryden

1967

Journal of Pharmaceutical Sciences

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Section: Methods Used I N Assessing the Beneficial A N D Adverse Effementioning

confidence: 99%

Tissue Culture in the Study of the Effects of Drugs

Dawson

Dryden

1967

Journal of Pharmaceutical Sciences

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Biochemical studies of human tumors. II.In vitro estimation of individual tumor sensitivity to anticancer agents

Bickis

Henderson

Quastel

1966

Cancer

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Slices from fresh human tumor specimens were incubated in presence of radioactive tracers and a spectrum of anticancer drugs. The drug effects were estimated from the changed rates of tracer incorporation into DNA, RNA, and protein. In a group of some 40 tumors analyzed the response of each tumor was highly individualistic although the observed effects were consistent with the known modes of action of respective drugs. Nevertheless, they could be classified broadly as: 1) tumors resistant to all drugs, 2) tumors sensitive to most drugs and 3) tumors selectively sensitive to one or few drugs and resistant against others. Case histories are given, which demonstrate the generally good correlation between in vitro and in vivo tumor sensitivities.

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The growth of tumors in matrix cultures

Kaluscar

Ghidoni

Oneal

1968

Cancer

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Explants of 160 human benign and malignant tumors were cultured on the matrix of human fibrin foam. This fine porous spongy matrix prepared from human fibrinogen is biologically suitable for human tissue cultures without further preparation. Tumor cultures were maintained in the culture from 3 to 21 days. Fifty‐five benign tumors preserved the architecture in “mother” fragments but did not proliferate into the matrix. One hundred five malignant tumors showed various degrees of matrix invasion. In the group of 72 gastro‐intestinal tract adenocarcinomas the degree of differentiation in biopsy correlated with tumor cell invasion in the matrix culture. The observations suggest that the tumors in matrix cultures express morphologic and biologic characteristics in vitro similar to those in vivo. This method may have potential usefulness in evaluation of tumors in biopsy material as a guide to prognosis and treatment.

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Drug Assays on Organ Cultures of Biopsies from Human Tumours

Cited by 20 publications

References 7 publications

Tissue Culture in the Study of the Effects of Drugs

Tissue Culture in the Study of the Effects of Drugs

Biochemical studies of human tumors. II.In vitro estimation of individual tumor sensitivity to anticancer agents

The growth of tumors in matrix cultures

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