Drought Tolerant Wheat Varieties Enrich Seed Microbiomes For Beneficial Microbes Under Drought Conditions

Hone, Holly; Mann, Ross; Yang, Guodong; Kaur, Jatinder; Tannenbaum, Ian; Li, Tongda; Spangenberg, Germán; Sawbridge, Tim

doi:10.21203/rs.3.rs-113919/v1

Cited by 4 publications

(6 citation statements)

References 60 publications

(94 reference statements)

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“…In agreement with this, bacterial communities in L. multiflorum seeds were dominated by members from the Proteobacteria, including the above-mentioned genera and a few other groups (e.g., Erwinia spp.). This pattern of bacterial diversity in L. multiflorum seeds is similar to those documented in seeds from other grass species such as Lolium perenne (Tannenbaum et al, 2020(Tannenbaum et al, , 2021, Elymus nutans (Guo et al, 2021), Oryza sativa (Eyre et al, 2019;Wang et al, 2020a) and Triticum aestivum (Hone et al, 2021;Walsh et al, 2021). Several beneficial bacteria within the Pantoea and Pseudomonas genera have been isolated from plant seeds (Feng et al, 2006;Díaz Herrera et al, 2016;Verma et al, 2018).…”

Section: Discussionsupporting

confidence: 75%

Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities

et al. 2022

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Seeds commonly harbour diverse bacterial communities that can enhance the fitness of future plants. The bacterial microbiota associated with mother plant’s foliar tissues is one of the main sources of bacteria for seeds. Therefore, any ecological factor influencing the mother plant’s microbiota may also affect the diversity of the seed’s bacterial community. Grasses form associations with beneficial vertically transmitted fungal endophytes of genus Epichloë. The interaction of plants with Epichloë endophytes and insect herbivores can influence the plant foliar microbiota. However, it is unknown whether these interactions (alone or in concert) can affect the assembly of bacterial communities in the produced seed. We subjected Lolium multiflorum plants with and without its common endophyte Epichloë occultans (E+, E-, respectively) to an herbivory treatment with Rhopalosiphum padi aphids and assessed the diversity and composition of the bacterial communities in the produced seed. The presence of Epichloë endophytes influenced the seed bacterial microbiota by increasing the diversity and affecting the composition of the communities. The relative abundances of the bacterial taxa were more similarly distributed in communities associated with E+ than E- seeds with the latter being dominated by just a few bacterial groups. Contrary to our expectations, seed bacterial communities were not affected by the aphid herbivory experienced by mother plants. We speculate that the enhanced seed/seedling performance documented for Epichloë-host associations may be explained, at least in part, by the Epichloë-mediated increment in the seed-bacterial diversity, and that this phenomenon may be applicable to other plant-endophyte associations.

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Section: Discussionsupporting

confidence: 75%

Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities

et al. 2022

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“…Several bacterial attributes which are involved in promoting drought tolerance include the ability to produce 1-aminocyclopropane-1carboxylate deaminase (ACCd), indole-3-acetic acid (IAA) and siderophores. ACCd acts by preventing ethylene from reaching inhibitory levels to allow proper root growth under water deficit, IAA regulates stomatal aperture and enhances root and shoot growth under drought, siderophores allow proper nutrient cycling under drought (Hone et al, 2021).…”

Section: Mitigation Of Drought Stressmentioning

confidence: 99%

“…The beneficial seed microbes were line-specific and responsive to environmental stress. The study indicated that seeds collected from stressed plants form an important resource to identify microbes with growth-promoting activity (Hone et al, 2021).…”

Section: Mitigation Of Drought Stressmentioning

confidence: 99%

Wheat Microbiome: Structure, Dynamics, and Role in Improving Performance Under Stress Environments

et al. 2022

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Wheat is an important cereal crop species consumed globally. The growing global population demands a rapid and sustainable growth of agricultural systems. The development of genetically efficient wheat varieties has solved the global demand for wheat to a greater extent. The use of chemical substances for pathogen control and chemical fertilizers for enhanced agronomic traits also proved advantageous but at the cost of environmental health. An efficient alternative environment-friendly strategy would be the use of beneficial microorganisms growing on plants, which have the potential of controlling plant pathogens as well as enhancing the host plant’s water and mineral availability and absorption along with conferring tolerance to different stresses. Therefore, a thorough understanding of plant-microbe interaction, identification of beneficial microbes and their roles, and finally harnessing their beneficial functions to enhance sustainable agriculture without altering the environmental quality is appealing. The wheat microbiome shows prominent variations with the developmental stage, tissue type, environmental conditions, genotype, and age of the plant. A diverse array of bacterial and fungal classes, genera, and species was found to be associated with stems, leaves, roots, seeds, spikes, and rhizospheres, etc., which play a beneficial role in wheat. Harnessing the beneficial aspect of these microbes is a promising method for enhancing the performance of wheat under different environmental stresses. This review focuses on the microbiomes associated with wheat, their spatio-temporal dynamics, and their involvement in mitigating biotic and abiotic stresses.

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“…Different seed-associated microbes were identified to protect crops against various biotic-abiotic stresses and enhance plant growth (Links et al, 2014;Mousa et al, 2016;Gdanetz and Trail, 2017;Shahzad et al, 2018;Li et al, 2020Li et al, , 2021Hone et al, 2021). In last two decades, the use of growth-promoting bacteria in agriculture has increased significantly to reduce the use of chemical fertilizers and enhance plant nutrient uptake (Rascovan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Notably, many crop, plant, and vegetable seeds were also reported to be inhabited by some of these bacterial genera (Johnston-Monje et al, 2016;Liu et al, 2017;Adam et al, 2018;Berg and Raaijmakers, 2018;Khalaf and Raizada, 2018;López et al, 2018;Wassermann et al, 2019a;Abdullaeva et al, 2021;Hone et al, 2021). However, despite the enormous potential of seed microbiomes to promote plant growth and sustainable agricultural practices, the impact of current international seed storage strategies on the seed microbial diversity and composition has not yet been evaluated (Berg and Raaijmakers, 2018).…”

Section: Introductionmentioning

confidence: 99%

Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes

et al. 2021

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Global seed vaults are important, as they conserve plant genetic resources for future breeding to improve crop yield and quality and to overcome biotic and abiotic stresses. However, little is known about the impact of standard storage procedures, such as seed drying and cold storage on the seed bacterial community, and the ability to recover seed-associated bacteria after storage. In this study, soybean [Glycine max (L.) Merr.] seeds were analyzed to characterize changes in the bacterial community composition and culturability under varying storage conditions. The G. max bacterial microbiome was analyzed from undried seed, dried seed, and seed stored for 0, 3, 6, and 14months. Storage temperatures consisted of −20°C, 4°C, and room temperature (RT), with −20°C being commonly used in seed storage vaults globally. The seed microbiome of G. max was dominated by Gammaproteobacteria under all conditions. Undried seed was dominated by Pantoea (33.9%) and Pseudomonas (51.1%); however, following drying, the abundance of Pseudomonas declined significantly (0.9%), Pantoea increased significantly (73.6%), and four genera previously identified including Pajaroellobacter, Nesterenkonia, env.OPS_17, and Acidibacter were undetectable. Subsequent storage at RT, 4, or −20°C maintained high-abundance Genera at the majority of time points, although RT caused greater fluctuations in abundances. For many of the low-abundance Genera, storage at −20°C resulted in their gradual disappearance, whereas storage at 4°C or RT resulted in their more rapid disappearance. The changes in seed bacterial composition were reflected by cultured bacterial taxa obtained from the stored G. max seed. The main taxa were largely culturable and had similar relative abundance, while many, but not all, of the low-abundance taxa were also culturable. Overall, these results indicate that the initial seed drying affects the seed bacterial composition, suggesting that microbial isolation prior to seed drying is recommended to conserve these microbes. The standard seed storage condition of −20°C is most suitable for conservation of the bacterial seed microbiome, as this storage temperature slows down the loss of seed bacterial diversity over longer time periods, particularly low-abundance taxa.

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Drought Tolerant Wheat Varieties Enrich Seed Microbiomes For Beneficial Microbes Under Drought Conditions

Cited by 4 publications

References 60 publications

Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities

Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities

Wheat Microbiome: Structure, Dynamics, and Role in Improving Performance Under Stress Environments

Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes

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