Drought-tolerant transgenic Swingle Citrumelo controls accumulation of proline modulating the expression of key genes of the proline metabolism

Barichello, Diliane; Farinacio, Renato; Santos, Camilla Carvalho Numes dos; Andrade, Giselly Aparecida; Nagashima, Getúlio Takashi; Garbúglio, Deoclécio Domingues; Yada, Inês Fumiko Ubukata; Travensolo, Regiane de Fátima; Bianchi, Beatriz Cutilak; Vieira, Luiz Gonzaga Esteves; Carlos, Eduardo Fermino

doi:10.4322/crt.icc102

Cited by 3 publications

(1 citation statement)

References 18 publications

(23 reference statements)

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“…Abiotic stresses, such as drought and salinity, are also important problems faced in citrus orchards. In this case, genes coding for osmoprotectants ( Longhi et al., 2022 ; Barichello et al., 2017 ) or transcriptional factors ( Romero-Romero et al., 2020 ) can be interesting for genetic transformation or gene editing. In the field, modulation of genes involved in the architecture of the canopy and fruit quality ( Dutt et al., 2022 ) are also interesting strategies to facilitate management and commercialization of citrus fruits.…”

Section: Gene Editing Of Citrusmentioning

confidence: 99%

CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane

Prado,

Rocha,

Santos

et al. 2024

Front. Plant Sci.

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Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars.

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