Drought tolerance in citrus rootstocks is associated with better antioxidant defense mechanism

Hussain, Sajjad; Khalid, Muhammad Fasih; Saqib, Muhammad; Ahmad, Shakeel; Zafar, Waseem; Rao, Muhammad Junaid; Morillón, Raphaël; Anjum, Muhammad Akbar

doi:10.1007/s11738-018-2710-z

Cited by 86 publications

(58 citation statements)

References 46 publications

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“…In conclusion, the wild type plants were more prone to superoxide radicals and ROS damage during drought stress. Drought-tolerant citrus varieties showed significantly higher antioxidant enzymatic activities and antioxidant capacity [20,[45][46][47] than the wild type plants; a significant increment in antioxidant enzymatic activities is correlated with drought tolerance in citrus [20]. Thus, our results demonstrated that the overexpression of the CsCYT75B1 gene contributes to drought tolerance via the high accumulation of antioxidant flavonoids with better antioxidant enzymatic activities (free radical scavenging capability) in all transgenic Arabidopsis lines.…”

Section: Accumulation Of Antioxidant Flavonoids In Overexpressed Linementioning

confidence: 51%

“…Drought stress has been shown to confer a significant degradation of photosynthetic pigments such as chlorophyll a and b contents in the leaves of Amaranthus tricolor, with a reduced overall efficacy of the photosynthetic apparatus [64]. In another study, the same results were observed in different citrus rootstocks; chlorophyll a and b degraded as the drought stress was prolonged in the leaves of various citrus rootstocks [20]. Correspondingly, other abiotic stresses such as high light stress in poinsettia (Euphorbia pulcherrima) plants damage chlorophyll a and b [59].…”

Section: Discussionmentioning

confidence: 64%

“…Among citrus rootstocks, the genotype that maintain highest antioxidant enzymatic activity (SOD, POD, and CAT) with a higher total antioxidant activity and capacity during drought stress are considered to be drought-tolerant, and the genotype that possesses the lower antioxidant enzymatic activity is considered to be drought-sensitive-that genotype has shown serve drought symptoms on leaves [20]. The total antioxidant activity and capacity increases significantly after drought stress in Solanum scabrum Mill and Solanum scabrum [57].…”

Section: Discussionmentioning

confidence: 99%

“…Varied levels of secondary metabolites are present among diverse citrus germplasm, and some have high levels of antioxidant flavonoids such as Citrus grandis, Citrus medica, and Poncirus trifoliata [16], and, interestingly, these citrus species have shown a high expression of P450s genes [13,14]; in addition, these citrus germplasm confer a tolerance against biotic [16] and abiotic stresses [20,21]. However, the biosynthesis mechanism of these antioxidant flavonoids and the regulation of P450s genes are less studied in citrus [6].…”

Section: Introductionmentioning

confidence: 99%

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CsCYT75B1, a Citrus CYTOCHROME P450 Gene, Is Involved in Accumulation of Antioxidant Flavonoids and Induces Drought Tolerance in Transgenic Arabidopsis

Rao

Tang

et al. 2020

Antioxidants

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CYTOCHROME P450s genes are a large gene family in the plant kingdom. Our earlier transcriptome data revealed that a CYTOCHROME P450 gene of Citrus sinensis (CsCYT75B1) was associated with flavonoid metabolism and was highly induced after drought stress. Here, we characterized the function of CsCYT75B1 in drought tolerance by overexpressing it in Arabidopsis thaliana. Our results demonstrated that the overexpression of the CsCYT75B1 gene significantly enhanced the total flavonoid contents with increased antioxidant activity in transgenic Arabidopsis. The gene expression results showed that several genes that are responsible for the biosynthesis of antioxidant flavonoids were induced by 2-12 fold in transgenic Arabidopsis lines. After 14 days of drought stress, all transgenic lines displayed an enhanced tolerance to drought stress along with accumulating antioxidant flavonoids with lower superoxide radicals and reactive oxygen species (ROS) than wild type plants. In addition, drought-stressed transgenic lines possessed higher antioxidant enzymatic activities than wild type transgenic lines. Moreover, the stressed transgenic lines had significantly lower levels of electrolytic leakage than wild type transgenic lines. These results demonstrate that the CsCYT75B1 gene of sweet orange functions in the metabolism of antioxidant flavonoid and contributes to drought tolerance by elevating ROS scavenging activities.

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Section: Accumulation Of Antioxidant Flavonoids In Overexpressed Linementioning

confidence: 51%

Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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CsCYT75B1, a Citrus CYTOCHROME P450 Gene, Is Involved in Accumulation of Antioxidant Flavonoids and Induces Drought Tolerance in Transgenic Arabidopsis

Rao

Tang

et al. 2020

Antioxidants

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“…Chlorophyll a and b were decreased dramatically in wild type and empty vector lines after 14 days of HLS; but all the transgenic lines maintained high level of chlorophyll a and b than wild type ( Figure 4A, B). Rapid degradation of chlorophyll, due to environmental stresses shows that the plants are more prone to oxidative stress with lower light harvesting capability [34,35]. Our results showed rapid degradation of chlorophyll a and b in wild type plants while all transgenic lines possess high level of chlorophyll a and b after 14 days of HLS; which clearly showed that wild type plants were under high stress and more prone to free radical damage than transgenic Arabidopsis lines ( Figure 4A).…”

Section: Metabolic Response Of Transgenic Arabidopsis Against Light Smentioning

confidence: 58%

Overexpression of citrus UDP-GLUCOSYL TRANSFERASE gene enhances anthocyanin and proanthocyanidins contents and confers high light tolerance

Rao

Huang

et al. 2019

Preprint

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Background Citrus fruits are consumed freshly or as juice to directly provide various dietary flavonoids to humans. Diverse metabolites at different levels are present among Citrus genera and many flavonoids biosynthetic genes were induced after abiotic stresses. To better understand the underlying mechanism, we designed experiments to overexpress a UDP-GLUCOSYL TRANSFERASE gene from Citrus sinensis (sweet orange) to evaluate its possible function in metabolism and response to stress.Results Our results demonstrated that overexpression of Cs-UGT78D3 resulted in high accumulation of proanthocyanidins in the seed coat and a dark brown color to transgenic Arabidopsis seeds. In addition, the total contents of flavonoid and anthocyanin were significantly enhanced in the leaves of overexpressed lines. Gene expression analyses indicated that many flavonoid (flavonol) and anthocyanin genes were up-regulated by 4-15 folds in transgenic Arabidopsis. Moreover, after 14 days of high light stress treatment, the transgenic Arabidopsis lines showed strong antioxidant activity and higher total contents of anthocyanins and flavonoids in leaves compared with the wild type.Conclusion Our study concluded that the citrus Cs-UGT78D3 gene is involved in proanthocyanidins accumulation in seed coats and confers tolerance to high light stress by accumulating the total anthocyanin and flavonoid contents with better antioxidant potential (due to photoprotective activity of anthocyanin) in the transgenic Arabidopsis.

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Citrus Root and Rhizosphere Dynamics in the Age of HLB

Rossi

Hallman

Santiago

2021

Annual Plant Reviews Online

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Huanglongbing (HLB; also known as citrus greening) is severely reducing global citrus production. HLB is a bacterial disorder caused by the vector‐transmitted pathogen Candidatus Liberibacter asiaticus ( C Las), and, as of today, it is one of the most destructive diseases facing the citrus industry of Florida and beyond. HLB in Florida was first detected in 2005 and is now widely distributed throughout the commercial citrus‐growing regions in the state. Preliminary studies have found that root systems of HLB‐tolerant rootstocks show less damage in response to C Las infection, whereas marked injury is evident soon after infection of susceptible material. An HLB‐damaged root system has poor nutrient uptake capacity, which leads to nutrient deficiencies and impairs tree performance. As of today, plant mechanisms of HLB‐tolerance are still unclear and there is no cure for HLB and root structure–function relationships remain an understudied component of the responses and may play a key role in tolerance. This review summarised all the recent studies on soil and root health of HLB‐affected citrus. Better understanding of how roots and soils are affected by HLB can potentially lead to improved management practices.

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Drought tolerance in citrus rootstocks is associated with better antioxidant defense mechanism

Cited by 86 publications

References 46 publications

CsCYT75B1, a Citrus CYTOCHROME P450 Gene, Is Involved in Accumulation of Antioxidant Flavonoids and Induces Drought Tolerance in Transgenic Arabidopsis

CsCYT75B1, a Citrus CYTOCHROME P450 Gene, Is Involved in Accumulation of Antioxidant Flavonoids and Induces Drought Tolerance in Transgenic Arabidopsis

Overexpression of citrus UDP-GLUCOSYL TRANSFERASE gene enhances anthocyanin and proanthocyanidins contents and confers high light tolerance

Citrus Root and Rhizosphere Dynamics in the Age of HLB

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