Drought Stress Tolerance in Plants: Insights from Transcriptomic Studies

Kido, Éderson Akio; Ferreira‐Neto, José Ribamar Costa; Pandolfi, Valesca; Souza, Amanda Cordeiro de Melo; Benko‐Iseppon, Ana Maria

doi:10.1007/978-3-319-32423-4_6

Cited by 8 publications

(8 citation statements)

References 144 publications

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“…A solution of polyethylene glycol (PEG) can be used to induce drought stress that is measured using a timescale of days after treating the seeds with the PEG solution. There are many different concentrations of PEG, therefore, it is essential to test a wide range of concentrations [107]. PEG has a high molecular weight (6000 or 8000) and prevents water from penetrating the cell wall.…”

Section: Advances In Breeding For Drought Tolerancementioning

confidence: 99%

Drought Stress Tolerance in Wheat and Barley: Advances in Physiology, Breeding and Genetics Research

Sallam

Alqudah

Dawood

et al. 2019

IJMS

399

312

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Climate change is a major threat to most of the agricultural crops grown in tropical and sub-tropical areas globally. Drought stress is one of the consequences of climate change that has a negative impact on crop growth and yield. In the past, many simulation models were proposed to predict climate change and drought occurrences, and it is extremely important to improve essential crops to meet the challenges of drought stress which limits crop productivity and production. Wheat and barley are among the most common and widely used crops due to their economic and social values. Many parts of the world depend on these two crops for food and feed, and both crops are vulnerable to drought stress. Improving drought stress tolerance is a very challenging task for wheat and barley researchers and more research is needed to better understand this stress. The progress made in understanding drought tolerance is due to advances in three main research areas: physiology, breeding, and genetic research. The physiology research focused on the physiological and biochemical metabolic pathways that plants use when exposed to drought stress. New wheat and barley genotypes having a high degree of drought tolerance are produced through breeding by making crosses from promising drought-tolerant genotypes and selecting among their progeny. Also, identifying genes contributing to drought tolerance is very important. Previous studies showed that drought tolerance is a polygenic trait and genetic constitution will help to dissect the gene network(s) controlling drought tolerance. This review explores the recent advances in these three research areas to improve drought tolerance in wheat and barley.

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Section: Advances In Breeding For Drought Tolerancementioning

confidence: 99%

Drought Stress Tolerance in Wheat and Barley: Advances in Physiology, Breeding and Genetics Research

Sallam

Alqudah

Dawood

et al. 2019

IJMS

399

312

View full text Add to dashboard Cite

show abstract

“…For drought tolerance evaluation, due to the unpredictability of rainfall and soil heterogeneity of eld experiment, PEG solution has been widely employed as an alternative to simulate water shortage condition, especially for the trials at seedling stage [6,16,24]. In sesame, Li et al [16] used relative values of germination rate and fresh weight (the ratio of the traits value under stress conditions to the same traits under stress free conditions) to evaluate the drought tolerance of sesame at early seedling stage.…”

Section: Discussionmentioning

confidence: 99%

Construction of a High Density Genetic Map and QTL Mapping of PEG-Induced Drought Tolerance at Seedling Stage in Sesame Using Whole Genome Re-Sequencing

Liang

Yan

et al. 2020

Preprint

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BackgroundImprovement in sesame (Sesamum indicum L.) drought tolerance at seedling stage is important for yield stability. Genetic approaches combing with conventional breeding is the most effective way to develop drought-tolerant cultivars. So far, very few studies have been reported to reveal gene/ quantitative trait loci (QTL) controlling drought tolerance in sesame. To identify the genomic regions associated with drought tolerance, we constructed a high-density genetic map using a recombinant inbred line (RIL) population through whole genome re-sequencing (WGRS) technique. QTLs contributing to three seedling traits were identified under both non-stress and water stress conditions.ResultsThree drought tolerance related traits and their relative values (the ratio of value under stress to value under control condition), including seedling weight (SW), shoot length (SL) and root length (RL), were evaluated under control and PEG-induced osmotic conditions at seedling stage in a RIL population derived from cross of Zhushanbai (ZSB) and Jinhuangma (JHM). Significant variation and high broad sense heritability were observed for all traits except SW under stress condition in the population. With this population, a high-density linkage map with 1354 bin markers was constructed through WGRS strategy. Composite interval mapping analysis was performed for all the traits as well as their relative phenotypic data. A total of 34 QTLs were detected for these three traits under both conditions and their relative values, and 13 stable QTLs associated with seven traits could be revealed in two independent experiments, explaining on average, 4.95-16.26% of phenotypic variation for each QTL. Four of them contributed more than 10% of phenotypic variation. Root length related QTLs were first identified in sesame. One region on chromosome 12 contained two major QTLs related to RL under osmotic condition and relative RL. ConclusionThe current study reports the first QTL mapping of drought tolerance related traits through a RIL population and first QTL detection of root related trait (root length) in sesame. These findings will provide new genetic resources for molecular improvement of drought tolerance and candidate gene identification in sesame.

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“…To unravel the effects of water deficit in genetic networks, the use of a solution containing polyethylene glycol (PEG) in hydroponic culture is a common practice to induce osmotic stress and reduce the water potential of tissues in plants (Claeys and Inzé, 2013; Osmolovskaya et al, 2018). In this way, the transcriptome of PEG-treated plants provides information regarding drought-related genes, which can be primarily classified in protective and regulatory genes (Kido et al, 2016). Regarding the former, these are genes that encode LEA proteins, chaperones, osmoprotectants, water channels, ion exchangers, and enzymes involved in the osmolyte biosynthesis and the reactive oxygen species (ROS), among others (Chen et al, 2019; Riyazuddin et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…In this way, transcriptomics has provided new potential resources for studying the molecular response of abiotic stress in crops (Zhuang et al, 2014), being RNA sequencing (RNA-Seq) the general method of choice. This method allows a broad coverage of the transcriptome, providing a significant characterization of mRNA transcripts of specific tissue and time and, in addition, is a quantitative method that yields a digital gene expression atlas at a genomic scale (Kido et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiles of eggplant (Solanum melongena) and its wild relativeS. dasyphyllumunder different levels of osmotic stress provide insights into response mechanisms to drought

Villanueva

Vilanova

Plazas

et al. 2022

Preprint

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Defence mechanisms to abiotic stresses, like drought, are very broad and RNA sequencing (RNA-Seq) can help in understanding the complex responses triggered. In this study, we performed RNA-Seq of the transcriptomes of eggplant (Solanum melongena) and its related wild species (S. dasyphyllum) under two PEG concentrations (20% and 30%), two different times (after 0.5 h and 2 h of osmotic stress) and at two plant phenological stages (three and five true fully developed leaves). Solanum dasyphyllum was more tolerant to osmotic stress, and a differential expression pattern of drought-related genes was identified between the two species. Plants subjected to a higher osmotic potential, at a more adult stage and at a higher stress exposure time displayed a higher number of DEGs (differential expressed genes). Gene ontology (GO) enrichment analysis revealed that, compared to S. melongena, S. dasyphyllum triggered the regulation of a wide range of transcription factors (AP2/ERF, DREB, bZIP, WRKY and bHLH). In both species, the abscisic acid (ABA) signaling response pathway played a crucial role leading to stomatal closure. Other important pathways involved in abiotic stresses tolerance including flavonoid, carotenoid and phenylpropanoid biosynthesis, chlorophyll metabolism and photosynthesis pathway among others were found to have a relevant role under both moderate and severe osmotic stresses. Our results reveal that S. dasyphyllum is a potential source of genes for breeding resilient eggplant varieties.

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Drought Stress Tolerance in Plants: Insights from Transcriptomic Studies

Cited by 8 publications

References 144 publications

Drought Stress Tolerance in Wheat and Barley: Advances in Physiology, Breeding and Genetics Research

Drought Stress Tolerance in Wheat and Barley: Advances in Physiology, Breeding and Genetics Research

Construction of a High Density Genetic Map and QTL Mapping of PEG-Induced Drought Tolerance at Seedling Stage in Sesame Using Whole Genome Re-Sequencing

Transcriptome profiles of eggplant (Solanum melongena) and its wild relativeS. dasyphyllumunder different levels of osmotic stress provide insights into response mechanisms to drought

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