Drought stress during soybean seed filling affects storage compounds through regulation of lipid and protein metabolism

Nakagawa, Andressa C. S.; Itoyama, Haruka; Ariyoshi, Yuri; Ario, Nobuyuki; Tomita, Yoshihiko; Kondo, Yukari; Iwaya‐Inoue, Mari; Ishibashi, Yoshihiro

doi:10.1007/s11738-018-2683-y

Cited by 34 publications

(26 citation statements)

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“…), reducing kernel mass (Corbellini et al, 1997). Drought stress induces the expression of cysteine proteinase (CysP) genes in arabidopsis and soybean seeds (Koizumi et al, 1993;Nakagawa et al, 2018;Seki et al, 2001). We found that high temperature stress decreased the expression of GmCysP2, although it increased the expression of GmCysP1 at 7 DAT (Figure 4(f,g)).…”

Section: Resultsmentioning

confidence: 85%

“…Arabidopsis WRI1-overexpressor plants have enhanced expression of PK, BCCP2, KAS1, and other lipidbiosynthesis-related genes, while downregulation of WRI1 reduces their expression since WRI1 binds to the AW box in their promoter regions (Maeo et al, 2009;To et al, 2012). Under drought stress during seed filling, GmPK, GmBCCP2, and GmKAS1 are downregulated in maturing seeds (Nakagawa et al, 2018). Under high temperature conditions, however, GmPK expression decreased at 7 DAT, GmBCCP2 expression increased at 14 DAT, and GmKAS1 expression increased at 21 and 28 DAT (Figure 3(d-f)).…”

Section: Resultsmentioning

confidence: 99%

“…In the absence of sucrose, phosphoenolpyruvate carboxykinase (PEPCK) activity is positively correlated with arabidopsis seedling establishment as it supplies sucrose through lipid degradation (Rylott et al, 2003). Drought stress during seed filling enhanced the expression of GmACX1, GmACX2, GmACX3, GmACX4, GmMS, and GmPEPCK and decreased seed lipid content (Nakagawa et al, 2018). High temperature stress, however, reduced the expression of GmACX1-4 and GmPEPCK at 7 DAT (Figure 3(g-j)), that of GmMS at 28 DAT (Figure 3(k)), and that of GmACX4 a b Figure 1.…”

Section: Resultsmentioning

confidence: 99%

“…In the field, they are frequently associated with each other, and both can reduce yields (Obata et al, 2015;Zhou et al, 2017). Drought stress during seed maturation in soybean decreases the seed lipid and protein contents by reducing biosynthesis and promoting degradation (Nakagawa et al, 2018). Previous studies reported that seed oil and protein contents could change also according to the temperature imposed on soybean plants.…”

Section: Introductionmentioning

confidence: 99%

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High temperature during soybean seed development differentially alters lipid and protein metabolism

Nakagawa

Ario

Tomita

et al. 2020

Plant Production Science

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High temperatures during seed development can affect the seed yield and quality in many crops. Here, we analyzed how high temperature alters the main seed storage compounds (lipid and protein) in soybean. At five days after R5 stage (initial seed filling stage), soybean plants were treated with control (20/20ºC day/night) and high temperature (30/30ºC day/night). After treatment, immature seed was sampled, analyzed for lipid and protein contents and for expression of seed storage compounds related genes. High temperature during seed filling increased lipid content but decreased protein content, associating with yield reduction. It increased the expression of two genes related to seed lipid biosynthesis (GmBCCP2 and GmKAS1) and genes for a lipid biosynthesis regulator (GmWRI1) and its transcription factor (GmDREBL), and decreased the expression of genes related to lipid degradation such as GmACXs. High temperature downregulated genes related to seed storage protein (GmGy1, GmGy2, GmGy4, GmGy5 and Gmβ-conglycinin) and upregulated genes for cysteine and aspartate proteinases. Therefore, high temperature during seed filling preferentially accumulates lipid than protein content in seed, although seed yield reduction was associated with lower seed protein content in soybean. Our study provides insights for further improvements of soybean seed oil under abiotic stress such as heat stress.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High temperature during soybean seed development differentially alters lipid and protein metabolism

Nakagawa

Ario

Tomita

et al. 2020

Plant Production Science

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“…Considering that seed filling is the most demanding period of soybean for resources like light, water, and nutrients (Nakagawa et al., 2018; Tripathi, Rabara, Shulaev, Shen, & Rushton, 2015), less interception of solar radiation and less exploitation of the soil by the roots at low SRs (Rigsby & Board, 2003), probably result in low utilization of resources, impairing seed filling. This information is relevant and shows that although soybean expresses morphological modifications to maintain the number of seeds per area, drastic reductions in SR (Figures 2a and 2b) will result in lower TSW (Figure 2c).…”

Section: Resultsmentioning

confidence: 99%

Minimum optimal seeding rate for indeterminate soybean cultivars grown in the tropics

et al. 2020

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The minimum optimal seeding rate in soybean [Glycine max (L.) Merr.] is the least seeds required to achieve optimal yield. This is a function of the crop phenotypic plasticity in response to plant density. However, the effect of seeding rate reduction on the seed composition and yield of cultivars with contrasting branching potentials needs to be better elucidated. The objectives were to evaluate the impacts of reducing the seeding rate on production and to quantify the minimum optimal seeding rate for seed, oil, and protein yield in soybean cultivars with contrasting plant architectures. The field experiment was conducted for two growing seasons in a 5 × 2 factorial scheme in a randomized complete block design with five replications. The treatments consisted of five seeding rates (100, 80, 60, 40, and 20% of the recommended seeding rate) and two indeterminate cultivars (BRS 1010IPRO and NS 5959IPRO). Both cultivars showed a minimum optimal seeding rate below the recommended rate. Pods per m 2 and the number of seeds per pod were the yield components responsible for the compensatory effect in response to lowering the seeding rate. Thousand seed weight was the main yield component responsible for yield loss below the minimum optimal seeding rate. Both cultivars showed high branch yield in response to seeding rate reduction. BRS 1010IPRO has a higher potential for seeding rate reduction than NS 5959IPRO. The minimum optimal seeding rate for seed yield in NS 5959IPRO led to lower protein yield and higher oil yield.Abbreviations: GS, growing season; MOSR, minimum optimal seeding rate; SR, seeding rate.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Mechanisms of Maturation and Germination in Crop Seeds Exposed to Environmental Stresses with a Focus on Nutrients, Water Status, and Reactive Oxygen Species

Ishibashi

Yuasa

Iwaya‐Inoue

2018

Advances in Experimental Medicine and Biology

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Drought stress during soybean seed filling affects storage compounds through regulation of lipid and protein metabolism

Cited by 34 publications

References 41 publications

High temperature during soybean seed development differentially alters lipid and protein metabolism

High temperature during soybean seed development differentially alters lipid and protein metabolism

Minimum optimal seeding rate for indeterminate soybean cultivars grown in the tropics

Mechanisms of Maturation and Germination in Crop Seeds Exposed to Environmental Stresses with a Focus on Nutrients, Water Status, and Reactive Oxygen Species

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