DROUGHT-INDUCED BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE enhances drought tolerance in rice

Shim, Jae Sung; Jeong, Hye In; Bang, Seung Woon; Jung, Se Eun; Kim, Goeun; Kim, Youn Shic; Redillas, Mark Christian Felipe R.; Oh, Sangnam; Seo, Jun Sung; Kim, Ju‐Kon

doi:10.1093/plphys/kiac560

Cited by 20 publications

(11 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lysine, methionine, threonine and isoleucine are commonly referred to as aspartate-derived amino acids because they are synthesized from aspartate through a branched pathway [ 40 ]. Isoleucine, valine and leucine are collectively referred to as branched-chain amino acids (BCAAs) because of their short branched hydrophobic side chains [ 41 ]. In the aspartate-derived amino acid pathway and BCAAs biosynthesis pathway, the values of L-lysine and (2 S )-2-isopropylmalate were higher in the Jizhen-2-CK vs. Jizhen-2-D comparison than in the ZC9-3-CK vs. ZC9-3-D comparison, but the opposite pattern was observed for the values of BCAAs.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, drought stress resulted in the significant up-regulation of one OASTL gene ( MD03G1184200 ) and one SAMS gene ( MD16G1138300 ) only in ‘ZC9-3’, and one SAMS gene ( MD17G1165400 ) was significantly down-regulated and up-regulated in ‘Jizhen-2’ and ‘ZC9-3’, respectively. BCAAs can accumulate in large quantities in plants in response to drought stress, and this might enhance the drought tolerance of plants, given that BCAAs could serve as compatible osmolytes or alternative energy sources [ 41 , 61 , 62 ]. The increase in the content of BCAAs following drought stress was less pronounced in ‘Jizhen-2’ compared with ‘ZC9-3’.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Integrative physiological, metabolomic, and transcriptomic analysis reveals the drought responses of two apple rootstock cultivars

Li,

Liu,

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Background Drought is considered the main environmental factor restricting apple production and thus the development of the apple industry. Rootstocks play an important role in enhancing the drought tolerance of apple plants. Studies of the physiology have demonstrated that ‘ZC9-3’ is a strong drought-resistant rootstock, whereas ‘Jizhen-2’ is a weak drought-resistant rootstock. However, the metabolites in these two apple rootstock varieties that respond to drought stress have not yet been characterized, and the molecular mechanisms underlying their responses to drought stress remain unclear. Results In this study, the physiological and molecular mechanisms underlying differences in the drought resistance of ‘Jizhen-2’ (drought-sensitive) and ‘ZC9-3’ (drought-resistant) apple rootstocks were explored. Under drought stress, the relative water content of the leaves was maintained at higher levels in ‘ZC9-3’ than in ‘Jizhen-2’, and the photosynthetic, antioxidant, and osmoregulatory capacities of ‘ZC9-3’ were stronger than those of ‘Jizhen-2’. Metabolome analysis revealed a total of 95 and 156 differentially accumulated metabolites in ‘Jizhen-2’ and ‘ZC9-3’ under drought stress, respectively. The up-regulated metabolites in the two cultivars were mainly amino acids and derivatives. Transcriptome analysis revealed that there were more differentially expressed genes and transcription factors in ‘ZC9-3’ than in ‘Jizhen-2’ throughout the drought treatment. Metabolomic and transcriptomic analysis revealed that amino acid biosynthesis pathways play key roles in mediating drought resistance in apple rootstocks. A total of 13 metabolites, including L-α-aminoadipate, L-homoserine, L-threonine, L-isoleucine, L-valine, L-leucine, (2S)-2-isopropylmalate, anthranilate, L-tryptophan, L-phenylalanine, L-tyrosine, L-glutamate, and L-proline, play an important role in the difference in drought resistance between ‘ZC9-3’ and ‘Jizhen-2’. In addition, 13 genes encoding O-acetylserine-(thiol)-lyase, S-adenosylmethionine synthetase, ketol-acid isomeroreductase, dihydroxyacid dehydratase, isopropylmalate isomerase, branched-chain aminotransferase, pyruvate kinase, 3-dehydroquinate dehydratase/shikimate 5-dehydrogenase, N-acetylglutamate-5-P-reductase, and pyrroline-5-carboxylate synthetase positively regulate the response of ‘ZC9-3’ to drought stress. Conclusions This study enhances our understanding of the response of apple rootstocks to drought stress at the physiological, metabolic, and transcriptional levels and provides key insights that will aid the cultivation of drought-resistant apple rootstock cultivars. Especially, it identifies key metabolites and genes underlying the drought resistance of apple rootstocks.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Integrative physiological, metabolomic, and transcriptomic analysis reveals the drought responses of two apple rootstock cultivars

Li,

Liu,

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…Evidence from numerous studies shows that regulating the amino acid metabolism pathway is a successful strategy to enhance plants' viability in adverse conditions [60][61][62][63][64]. Notably, amino acids in plants enhance their drought resistance by affecting enzyme activity [65], and metabolomics provides evidence that amino acids play a crucial role in salt stress in plants [66]. The present study aimed to investigate the effect of exogenous small peptides and surfactants on amino acid metabolism in tea plants.…”

Section: Discussionmentioning

confidence: 99%

Enhancing the Adaptability of Tea Plants (Camellia sinensis L.) to High-Temperature Stress with Small Peptides and Biosurfactants

Chen,

Song,

et al. 2023

Plants

View full text Add to dashboard Cite

Tea plants are highly susceptible to the adverse effects of a high-temperature climate, which can cause reduced yield and quality and even lead to plant death in severe cases. Therefore, reducing the damage caused by high-temperature stress and maintaining the photosynthetic capacity of tea plants is a critical technical challenge. In this study, we investigated the impact of small oligopeptides (small peptides) and surfactants on the high-temperature-stress tolerance of tea plants. Our findings demonstrated that the use of small peptides and surfactants enhances the antioxidant capacity of tea plants and protects their photosynthetic system. They also induce an increase in gibberellin (GA) content and a decrease in jasmonic acid (JA), strigolactone (SL), auxin (IAA), and cytokinin (CTK) content. At the same time, small peptides regulate the metabolic pathways of diterpenoid biosynthesis. Additionally, small peptides and surfactants induce an increase in L-Carnosine and N-Glycyl-L-Leucine content and a decrease in (5-L-Glutamyl)-L-Amino Acid content, and they also regulate the metabolic pathways of Beta-Alanine metabolism, Thiamine metabolism, and Glutathione metabolism. In summary, small peptides and surfactants enhance the ability of tea plants to resist high-temperature stress.

show abstract

“…Osmotic-stress-induced accumulation of BCAAs is dependent on ABA-regulated protein degradation in A. thaliana , unlike the proline accumulation that is due to de novo synthesis [ 49 ]. In rice, DROUGHT-INDUCED BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE (OsDIAT) mediates the accumulation of BCAAs in response to drought stress; and rice plants overexpressing OsDIAT have increased accumulation of BCAAs and are more tolerant to drought stress [ 50 ]. Significant accumulation of BCAAs only in S. viridis suggests that C 4 monocots might have more efficient mechanism to cope with osmotic-stress occurring due to sulfate starvation.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional and metabolic profiling of sulfur starvation response in two monocots

Zenzen,

Cassol,

Westhoff

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Background Sulfur (S) is a mineral nutrient essential for plant growth and development, which is incorporated into diverse molecules fundamental for primary and secondary metabolism, plant defense, signaling, and maintaining cellular homeostasis. Although, S starvation response is well documented in the dicot model Arabidopsis thaliana, it is not clear if the same transcriptional networks control the response also in the monocots. Results We performed series of physiological, expression, and metabolite analyses in two model monocot species, one representing the C3 plants, Oryza sativa cv. kitaake, and second representing the C4 plants, Setaria viridis. Our comprehensive transcriptomic analysis revealed twice as many differentially expressed genes (DEGs) in S. viridis than in O. sativa under S-deficiency, consistent with a greater loss of sulfur and S-containing metabolites under these conditions. Surprisingly, most of the DEGs and enriched gene ontology terms were species-specific, with an intersect of only 58 common DEGs. The transcriptional networks were different in roots and shoots of both species, in particular no genes were down-regulated by S-deficiency in the roots of both species. Conclusions Our analysis shows that S-deficiency seems to have different physiological consequences in the two monocot species and their nutrient homeostasis might be under distinct control mechanisms.

show abstract

DROUGHT-INDUCED BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE enhances drought tolerance in rice

Cited by 20 publications

References 55 publications

Integrative physiological, metabolomic, and transcriptomic analysis reveals the drought responses of two apple rootstock cultivars

Integrative physiological, metabolomic, and transcriptomic analysis reveals the drought responses of two apple rootstock cultivars

Enhancing the Adaptability of Tea Plants (Camellia sinensis L.) to High-Temperature Stress with Small Peptides and Biosurfactants

Transcriptional and metabolic profiling of sulfur starvation response in two monocots

Contact Info

Product

Resources

About