Drought and freezing vulnerability of the isolated hybrid aspen Populus x smithii relative to its parental species, P. tremuloides and P. grandidentata

Deacon, Nicholas J.; Grossman, Jake J.; Cavender‐Bares, Jeannine

doi:10.1002/ece3.5364

Cited by 9 publications

(12 citation statements)

References 56 publications

(87 reference statements)

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“…S6). This event was inferred from P. grandidentata to P. tremuloides and is consistent with previous reports of extensive hybridization and introgression between these two species (Deacon, et al 2019). A second migration edge was inferred from P. adenopoda to P. rotundifolia , which allowed us to explain 99.6% of the total variance (Fig.…”

Section: Resultssupporting

confidence: 92%

Drivers of genomic landscapes of differentiation acrossPopulusdivergence gradient

Shang

Rendón-Anaya

Paun

et al. 2021

Preprint

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Investigating genome-wide variation patterns along a speciation continuum is of central importance to understand the evolutionary processes contributing to lineage diversification. To identify which forces have shaped the genomic landscapes in Populus, we resequenced 201 whole-genomes from eight closely related species, with pairs of species at different stages along the speciation continuum. Using population structure and identity by descent analyses, we show extensive introgression between some species pairs, especially those with parapatric distributions. Inference of historical changes in effective population sizes support species-specific demographic trajectories, including recent population expansions in species characterized by broad present-day distributions. We observe highly conserved genomic landscapes, either focusing on within-species (genetic diversity: π and recombination rate: ρ) or among-species variation (relative divergence: FST and absolute divergence: DXY ). Independent of the stage across the divergence continuum, we recovered positive correlations between the pair π and ρ and the pair DXY and ρ across all species pairs, which is consistent with a substantial contribution of linked selection in shaping these genomic landscapes. However, the positive correlations between π and DXY became weaker as the overall divergence level (da) increased, suggesting that background selection is not the only factor at play. Positive correlations between FST and DXY in all species pairs, regardless of the rate of gene flow, also indicates the high FST could be due to divergent sorting of ancient polymorphism before speciation. Our study showcases the importance of investigating genomic patterns on multiple species across the speciation continuum to better understand the genomic landscapes of diversity and differentiation.

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Section: Resultssupporting

confidence: 92%

Drivers of genomic landscapes of differentiation acrossPopulusdivergence gradient

Shang

Rendón-Anaya

Paun

et al. 2021

Preprint

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“…Specifically, based on a modeling analysis (see Figure S1 in Supporting Information ), we show that the effective whole‐plant P50 is more likely to be lower than the segment P50 in the roots, and higher in the segments that are further away from the soil (i.e., closer to leaves). The P50 data for the other species are scarce (three data points for paper birch (Kattge et al., 2020); one for bigtooth aspen (Deacon et al., 2019), and none for white pine), although our estimates of P50 for paper birch and bigtooth aspen are still consistent with measurements.…”

Section: Resultssupporting

confidence: 65%

“…(b) Measurements from the literature (data for red maple and paper birch are from the TRY database (Kattge et al., 2020); data for bigtooth aspen are from Deacon et al. (2019); no data have been found for white pine).…”

Section: Resultsmentioning

confidence: 99%

Intra‐Specific Variability in Plant Hydraulic Parameters Inferred From Model Inversion of Sap Flux Data

Sloan

Thompson

et al. 2022

JGR Biogeosciences

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Understanding plant hydraulic regulation is critical for predicting plant and ecosystem responses to projected increases in drought stress. Plant hydraulic regulation is controlled by observable, diverse plant hydraulic traits that can vary as much across individuals of the same species as they do across different species. Direct measurements of plant hydraulic traits from a range of ecosystems remain limited in comparison to other, more readily measured traits (e.g., specific leaf area). Furthermore, plant hydraulic trait measurements, often made at leaf or branch levels, are not easily scaled to whole‐plant values that are typically used to predict plant and ecosystem fluxes. In this study, multiple whole‐plant hydraulic parameters are inferred from observations of plant water use (i.e., sap flow), soil properties, and meteorological data. We use a Markov Chain Monte Carlo model inversion approach to obtain the best estimates and uncertainty of plant hydraulic parameters that capture whole‐plant effective embolism resistance and stomatal sensitivity to decreasing plant water potential. We then use the inferred values in the model to estimate whole‐tree water use and isohydricity. This approach reliably infers whole‐plant parameter values with enough specificity to resolve inter‐ and intra‐specific differences, and thus supplements time‐ and labor‐intensive direct measurements of traits.

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“…However, it has remained unclear whether freezing at these temperatures is actually sufficient to relativize leakage across samples varying in size and anatomy [26]. Furthermore, the temperature at which samples are boiled or heated and/or the time during which this temperature is reached are frequently not specified ( [10,12,17,[27][28][29], among others). And though autoclaving Fig.…”

Section: What Is the Best Way To Standardize Measurements Of Freezingmentioning

confidence: 99%

Standardization of electrolyte leakage data and a novel liquid nitrogen control improve measurements of cold hardiness in woody tissue

Kovaleski

Grossman

2021

Plant Methods

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Background A variety of basic and applied research programs in plant biology require the accurate and reliable determination of plant tissue cold hardiness. Over the past 50 years, the electrolyte leakage method has emerged as a popular and practical method for quantifying the amount of damage inflicted on plant tissue by exposure to freezing temperatures. Numerous approaches for carrying out this method and analyzing the resultant data have emerged. These include multiple systems for standardizing and modeling raw electrolyte leakage data and multiple protocols for boiling or autoclaving samples in order to maximize leakage as a positive control. We compare four different routines for standardization of leakage data and assess a novel control method—immersion in liquid nitrogen in lieu of traditional autoclaving—and apply them to woody twigs collected from 12 maple (Acer) species in early spring. We compare leakage data from these samples using each of four previously published forms of data analysis and autoclaving vs. liquid nitrogen controls and validate each of these approaches against visual estimates of freezing damage and differential thermal analysis. Results Through presentation of our own data and re-analysis of previously published findings, we show that standardization of raw data against estimates of both minimum and maximum attainable freezing damage allows for reliable estimation of cold hardiness at the species level and across studies in diverse systems. Furthermore, use of our novel liquid nitrogen control produces data commensurate across studies and enhances the consistency and realism of the electrolyte leakage method, especially for very cold hardy samples. Conclusion Future leakage studies that relativize data against minimum and maximum leakage and that employ our updated liquid nitrogen control will contribute generalizable, repeatable, and realistic data to the existing body of cold hardiness research in woody plants. Data from studies conducted using a liquid nitrogen (and not an autoclaving) control can still be compared to previously published data, especially when raw data are standardized using the best-performing approach among those we assessed. Electrolyte leakage of woody twigs emerges as a useful technique for quickly assessing the probability of tissue death in response to freezing in dormant plants. Differential thermal analysis may provide different and complementary information on cold hardiness.

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Drought and freezing vulnerability of the isolated hybrid aspen Populus x smithii relative to its parental species, P. tremuloides and P. grandidentata

Cited by 9 publications

References 56 publications

Drivers of genomic landscapes of differentiation acrossPopulusdivergence gradient

Drivers of genomic landscapes of differentiation acrossPopulusdivergence gradient

Intra‐Specific Variability in Plant Hydraulic Parameters Inferred From Model Inversion of Sap Flux Data

Standardization of electrolyte leakage data and a novel liquid nitrogen control improve measurements of cold hardiness in woody tissue

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