Drosophila salivary glands exhibit a regional reprogramming of gene expression during the third larval instar

Thüroff, Eduardo; Stöven, Svenja; Kress, Horst

doi:10.1016/0925-4773(92)90017-e

Cited by 9 publications

(3 citation statements)

References 34 publications

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“…Hybridization and staining on chromosomes and wholemount hybridization on Drosophila third larval instar salivary glands were performed according to [19]. In situ hybridization on Drosophila embryos was performed using a modified version of the Tautz and Pfeifle protocol [20].…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Functional regulation of glutamine:fructose-6-phosphate aminotransferase 1 (GFAT1) of Drosophila melanogaster in a UDP-N-acetylglucosamine and cAMP-dependent manner

Graack

Cinque

Kress

2001

Biochemical Journal

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Glutamine:fructose-6-phosphate aminotransferase (GFAT; EC 2.6.1.16) expression is tightly regulated in the context of amino sugar synthesis in many organisms from yeast to humans by transcriptional and post-translational processes. We have cloned the cDNA of the GFAT1 of Drosophila melanogaster (Dmel/Gfat1). One of the two putative protein kinase A (PKA) phosphorylation sites proposed for the regulation of human GFAT1 [Zhou, Huynh, Hoffmann, Crook, Daniels, Gulve and McClain (1998) Diabetes 47, 1836–1840] is conserved in Dmel/GFAT1. In the other one the reactive serine has been converted to a cysteine, making further access by PKA unlikely. The Dmel/Gfat1 gene is localized at position 81F on the right arm of chromosome 3. By whole-mount in situ hybridization specific expression of Dmel/GFAT1 was detected in embryonic chitin-synthesizing tissues and in the corpus cells of salivary glands from late third larval instar. Expressing Dmel/GFAT1 in yeast we showed that Dmel/GFAT1 activity is controlled by UDP-N-acetylglucosamine and PKA in the yeast total protein extract system. We propose a model for the independent regulation of the Dmel/GFAT1 enzyme by feedback inhibition and PKA.

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Section: In Situ Hybridizationmentioning

confidence: 99%

Functional regulation of glutamine:fructose-6-phosphate aminotransferase 1 (GFAT1) of Drosophila melanogaster in a UDP-N-acetylglucosamine and cAMP-dependent manner

Graack

Cinque

Kress

2001

Biochemical Journal

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“…For in situ hybridization on polytene chromosomes DNA probes (0.5-1 µg) were labelled with biotin (BioNick4 Labelling System ; Life Technologies) and checked for functionality on a Southern blot. Hybridization and staining on chromosomes and wholemount hybridization on Drosophila third larval instar salivary glands were performed according to [19]. In situ hybridization on Drosophila embryos was performed using a modified version of the Tautz and Pfeifle protocol [20].…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Functional regulation of glutamine:fructose-6-phosphate aminotransferase 1 (GFAT1) of Drosophila melanogaster in a UDP-N-acetylglucosamine and cAMP-dependent manner

2001

Self Cite

View full text Add to dashboard Cite

Glutamine:fructose-6-phosphate aminotransferase (GFAT; EC 2.6.1.16) expression is tightly regulated in the context of amino sugar synthesis in many organisms from yeast to humans by transcriptional and post-translational processes. We have cloned the cDNA of the GFAT1 of Drosophila melanogaster (Dmel/Gfat1). One of the two putative protein kinase A (PKA) phosphorylation sites proposed for the regulation of human GFAT1 [Zhou, Huynh, Hoffmann, Crook, Daniels, Gulve and McClain (1998) Diabetes 47, 1836-1840] is conserved in Dmel/GFAT1. In the other one the reactive serine has been converted to a cysteine, making further access by PKA unlikely. The Dmel/Gfat1 gene is localized at position 81F on the right arm of chromosome 3. By whole-mount in situ hybridization specific expression of Dmel/GFAT1 was detected in embryonic chitin-synthesizing tissues and in the corpus cells of salivary glands from late third larval instar. Expressing Dmel/GFAT1 in yeast we showed that Dmel/GFAT1 activity is controlled by UDP-N-acetylglucosamine and PKA in the yeast total protein extract system. We propose a model for the independent regulation of the Dmel/GFAT1 enzyme by feedback inhibition and PKA.

show abstract

“…In vitro transcription products were hybridized in 6x SSPE (60 mM Na 2 HPO 4 , pH 7.4, 15 mM Na-acetate, 3 mM EDTA), 0.5% SDS, 500 lg/ml tRNA and 10% PEG 6000 O/N at 68 v C. In situ hybridizations of biotinylated probes to polytene chromosomes and whole mount in situ hybridizations of DIG-labeled probes were done as described (Thü roff et al, 1992).…”

Section: Hybridizationsmentioning

confidence: 99%

A Kunitz type protease inhibitor related protein is synthesized in Drosophila prepupal salivary glands and released into the moulting fluid during pupation

Kress

Jarrin

Thüroff

et al. 2004

Insect Biochemistry and Molecular Biology

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Drosophila salivary glands exhibit a regional reprogramming of gene expression during the third larval instar

Cited by 9 publications

References 34 publications

Functional regulation of glutamine:fructose-6-phosphate aminotransferase 1 (GFAT1) of Drosophila melanogaster in a UDP-N-acetylglucosamine and cAMP-dependent manner

Functional regulation of glutamine:fructose-6-phosphate aminotransferase 1 (GFAT1) of Drosophila melanogaster in a UDP-N-acetylglucosamine and cAMP-dependent manner

Functional regulation of glutamine:fructose-6-phosphate aminotransferase 1 (GFAT1) of Drosophila melanogaster in a UDP-N-acetylglucosamine and cAMP-dependent manner

A Kunitz type protease inhibitor related protein is synthesized in Drosophila prepupal salivary glands and released into the moulting fluid during pupation

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